Method for detecting TaAGPS gene allelic variation relevant to thousand kernel weight

An allelic variation and 1000-grain weight technology, applied in the field of in situ variation, can solve the problems of reduced endosperm starch accumulation, no instantaneous starch synthesis, and reduced AGPase enzyme activity, achieving the effect of easy operation.
CN105907862AInactive Publication Date: 2016-08-31INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
2 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Publication Date
2016-08-31
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for detecting wheat cytoplasmic adp glucose pyrophosphorylase small subunit (TaAGPS) gene excellent allelic variation relevant to thousand kernel weight by using CAPS (cleaved amplified polymorphic sequence) marks. According to the method, the corresponding basic groups of two allelic variations (TaAGPS-7A-T and TaAGPS-7A-G) in the 5092 site of the TaAGPS-7A exon region III are respectively T and G; the corresponding amino acid is Ser and Ala; firstly, a primer is used for performing PCR (polymerase chain reaction) amplification on SEQ ID No: 1 and SEQ ID No: 2; after the enzyme digestion by using DdeI incision enzyme, electrophoresis detection is performed; the authentication of different allelic variations of the TaAGPS genes in wheat germplasm resources and later generation breeding can be conveniently and efficiently realized. The operation can be completed through conventional PCR and agarose gel electrophoresis methods; the operation is convenient; stability and feasibility are realized.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for using CAPS (Cleaved Amplified Polymorphic Sequence) markers to detect the superior allelic variation of TaAGPS gene related to thousand-grain weight. Background technique

[0002] The genome of wheat is an allohexaploid, consisting of three highly homologous genomes, A, B, and D. The genome is large and complex, which provides a challenge for the development of wheat genetics and breeding research. Wheat is one of the most important food crops in the world and is widely grown around the globe. Studies such as Rajaram show that: with the increase of the world population, it is expected that the global demand for wheat will further increase by 40% in 2020, so cultivating high-yield wheat varieties has become one of the main breeding goals of current breeders. The yield of wheat is composed of three elements: the number of spikelets, the number of grains ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More