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Method for synthesizing arbutin by catalyzing through immobilized biological complex enzyme

A compound enzyme-catalyzed synthesis of arbutin and arbutin, which is applied in the direction of immobilization on or in the inorganic carrier, multi-enzyme system, fermentation, etc., can solve the problems of high cost, environmental pollution, low enzyme activity, etc., and achieve Increased yield, simple and easy-to-obtain raw materials, and high activity

Inactive Publication Date: 2016-09-07
郭舒洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: for the preparation of arbutin by the commonly used chemical synthesis method at present, not only the process steps are complicated, the reaction conditions are reliable, but also a large amount of toxic solvents are used, which seriously pollutes the environment. Due to the current situation of high cost and low enzyme activity, a kind of immobilized biological composite enzyme is prepared by extracting enzyme liquid and nano-attapulgite with white jade snail as raw material, and using it to catalyze the synthesis reaction of hydroquinone and glucose, and finally get arbutin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] First select 20 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 1cm, then add 5mL of rapeseed oil dropwise to the water, stir well and let stand for starvation treatment for 5 days, then take out the treated snails and shell them Dissect and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, oscillate ultrasonically with a power of 200W for 20min, filter and separate to remove the filter residue, and obtain the filtrate. Re-operate the filter residue according to the above steps, separate the filtrate, and combine the two filtrates; then put the combined filtrate into a high-speed centrifuge, centrifuge at 10000r / min for 10min, separate the supernatant, and transfer the supernatant to Vacuum concentration tank, reduce the pressure to 900Pa, concentrate to 1 / 3 of the original volume of the supern...

example 2

[0024] First select 25 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 1.5cm, then add 8mL rapeseed oil dropwise to the water, stir evenly, let stand for starvation treatment for 6 days, then take out the treated snails, remove Dissect the shell and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, and ultrasonically oscillate for 30 minutes with a power of 250W, filter and separate to remove the filter residue, and obtain the filtrate. Then the filter residue was re-operated according to the aforementioned steps, the filtrate was separated, and the filtrate was combined twice; then the combined filtrate was put into a high-speed centrifuge, and centrifuged at a speed of 11000r / min for 13min, and the supernatant was separated. Put it into a vacuum concentration tank, reduce the pressure to 950Pa, and ...

example 3

[0027] First select 30 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 2cm, then add 10mL rapeseed oil dropwise to the water, stir well and let stand for starvation treatment for 7 days, then take out the treated snails and shell them Dissect and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, and ultrasonically oscillate for 40 minutes with a power of 300W, filter and separate to remove the filter residue to obtain the filtrate, and then The filter residue was re-operated according to the previous steps, the filtrate was separated, and the filtrates were combined twice; then the combined filtrate was placed in a high-speed centrifuge, centrifuged at a speed of 12000r / min for 15min, the supernatant was separated, and the supernatant was transferred to Vacuum concentration tank, reduce the pressure t...

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Abstract

The invention discloses a method for synthesizing arbutin by catalyzing through an immobilized biological complex enzyme and belongs to the field of biological catalysis. The method takes white jade snails as raw materials; an extracting enzyme solution and nano attapulgites are compounded to prepare the immobilized biological complex enzyme; the immobilized biological complex enzyme is used for catalyzing a synthetic reaction of p-dihydroxybenzene and glucose to finally obtain the arbutin. The method is simple in process; the white jade snails are used as the raw materials so that the production cost is saved; in an operation process, no toxic reagent is used; ursolic acid is synthesized by utilizing the immobilized biological complex enzyme; reaction conditions are moderate, a production period is shortened and the yield of the arbutin is improved, so that the large-scale production of the arbutin becomes possible.

Description

technical field [0001] The invention discloses a method for catalyzing and synthesizing arbutin with an immobilized biological compound enzyme, which belongs to the field of biocatalysis. Background technique [0002] Arbutin can significantly inhibit tyrosinase activity, reduce the deposition of tyrosinase in the skin, and have the effects of bleaching, preventing discoloration and freckle removal on the skin; it is an internationally recognized efficient and safe whitening that is being promoted It is an ideal additive for whitening, freckle removing and sunscreen cosmetics. [0003] Enzyme immobilization technology is a new biotechnology developed in the 1960s, and its practical application effect is remarkable in chemical industry, fermentation production, energy, medicine and other industries. It is a method of using physical or chemical means to locate the free enzyme in a limited space area and keep it active for repeated use. Compared with the traditional suspensio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12N11/18C12N11/14
CPCC12P19/44C12N11/14C12N11/18
Inventor 郭舒洋张明林茂平
Owner 郭舒洋
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