Method for synthesizing arbutin by catalyzing through immobilized biological complex enzyme

A compound enzyme-catalyzed synthesis of arbutin and arbutin, which is applied in the direction of immobilization on or in the inorganic carrier, multi-enzyme system, fermentation, etc., can solve the problems of high cost, environmental pollution, low enzyme activity, etc., and achieve Increased yield, simple and easy-to-obtain raw materials, and high activity

Inactive Publication Date: 2016-09-07
郭舒洋
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: for the preparation of arbutin by the commonly used chemical synthesis method at present, not only the process steps are complicated, the reaction conditions are reliable, but also a large amount of toxic solvents are used, which seriously pollutes the environment. Due to the current situation of high cost and low enzyme activity, a kind of immobilized biological composite enzyme is prepared by extracting enzyme liquid and nano-attapulgite with white jade snail as raw material, and using it to catalyze the synthesis reaction of hydroquinone and glucose, and finally get arbutin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] First select 20 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 1cm, then add 5mL of rapeseed oil dropwise to the water, stir well and let stand for starvation treatment for 5 days, then take out the treated snails and shell them Dissect and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, oscillate ultrasonically with a power of 200W for 20min, filter and separate to remove the filter residue, and obtain the filtrate. Re-operate the filter residue according to the above steps, separate the filtrate, and combine the two filtrates; then put the combined filtrate into a high-speed centrifuge, centrifuge at 10000r / min for 10min, separate the supernatant, and transfer the supernatant to Vacuum concentration tank, reduce the pressure to 900Pa, concentrate to 1 / 3 of the original volume of the supern...

example 2

[0024] First select 25 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 1.5cm, then add 8mL rapeseed oil dropwise to the water, stir evenly, let stand for starvation treatment for 6 days, then take out the treated snails, remove Dissect the shell and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, and ultrasonically oscillate for 30 minutes with a power of 250W, filter and separate to remove the filter residue, and obtain the filtrate. Then the filter residue was re-operated according to the aforementioned steps, the filtrate was separated, and the filtrate was combined twice; then the combined filtrate was put into a high-speed centrifuge, and centrifuged at a speed of 11000r / min for 13min, and the supernatant was separated. Put it into a vacuum concentration tank, reduce the pressure to 950Pa, and ...

example 3

[0027] First select 30 white jade snails and put them into a beaker, add water to the beaker until the water depth reaches 2cm, then add 10mL rapeseed oil dropwise to the water, stir well and let stand for starvation treatment for 7 days, then take out the treated snails and shell them Dissect and take out the digestive glands; then mix the snail digestive glands obtained above with deionized water at a solid-to-liquid ratio of 1:8, put them into an ultrasonic oscillator, and ultrasonically oscillate for 40 minutes with a power of 300W, filter and separate to remove the filter residue to obtain the filtrate, and then The filter residue was re-operated according to the previous steps, the filtrate was separated, and the filtrates were combined twice; then the combined filtrate was placed in a high-speed centrifuge, centrifuged at a speed of 12000r / min for 15min, the supernatant was separated, and the supernatant was transferred to Vacuum concentration tank, reduce the pressure t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for synthesizing arbutin by catalyzing through an immobilized biological complex enzyme and belongs to the field of biological catalysis. The method takes white jade snails as raw materials; an extracting enzyme solution and nano attapulgites are compounded to prepare the immobilized biological complex enzyme; the immobilized biological complex enzyme is used for catalyzing a synthetic reaction of p-dihydroxybenzene and glucose to finally obtain the arbutin. The method is simple in process; the white jade snails are used as the raw materials so that the production cost is saved; in an operation process, no toxic reagent is used; ursolic acid is synthesized by utilizing the immobilized biological complex enzyme; reaction conditions are moderate, a production period is shortened and the yield of the arbutin is improved, so that the large-scale production of the arbutin becomes possible.

Description

technical field [0001] The invention discloses a method for catalyzing and synthesizing arbutin with an immobilized biological compound enzyme, which belongs to the field of biocatalysis. Background technique [0002] Arbutin can significantly inhibit tyrosinase activity, reduce the deposition of tyrosinase in the skin, and have the effects of bleaching, preventing discoloration and freckle removal on the skin; it is an internationally recognized efficient and safe whitening that is being promoted It is an ideal additive for whitening, freckle removing and sunscreen cosmetics. [0003] Enzyme immobilization technology is a new biotechnology developed in the 1960s, and its practical application effect is remarkable in chemical industry, fermentation production, energy, medicine and other industries. It is a method of using physical or chemical means to locate the free enzyme in a limited space area and keep it active for repeated use. Compared with the traditional suspensio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12N11/18C12N11/14
CPCC12P19/44C12N11/14C12N11/18
Inventor 郭舒洋张明林茂平
Owner 郭舒洋
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap