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Nucleic acid extraction device and working method thereof

A nucleic acid extraction device and nucleic acid technology, which are applied in the fields of botanical equipment and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of surrounding pore site pollution, cross infection, and reaction liquid entering surrounding pore sites, etc. Achieve the effect of solving cross-contamination and reducing parallel operations

Active Publication Date: 2018-02-23
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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Problems solved by technology

However, cross-infection is sometimes found during the PCR detection process, which is believed to be related to the use of a 96-well plate as the test unit in the nucleic acid extraction device for the processing of each test link. This plate-type scheme uses parallel processing to process multiple samples. Due to the small hole spacing and small hole depth, it is easy to cause the reaction liquid to enter the surrounding holes, thereby causing pollution to the surrounding holes, and the parallel suction and discharge of multiple heads can easily lead to a high risk of cross-contamination. In addition, Vibration and mixing of the whole plate is also easy to cause cross-contamination
[0005] Due to the exponential amplification of nucleic acid by PCR technology, the sensitivity of the test is very high, and the linear range is very wide. Therefore, PCR technology has very high requirements for cross-contamination, and the plate-type scheme has the aforementioned important defects in avoiding cross-contamination. This is also an important reason that restricts the clinical application of the program

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  • Nucleic acid extraction device and working method thereof
  • Nucleic acid extraction device and working method thereof
  • Nucleic acid extraction device and working method thereof

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Embodiment approach

[0026] According to one embodiment of the present invention, a nucleic acid extraction device is provided, which is used to realize the complete automation of the nucleic acid extraction process, that is, multiple testing links involved in a nucleic acid extraction process (such as including dispensing reaction solution, mixing and incubation) , magnetic separation and elution, etc.) are all automated. The nucleic acid extraction device includes:

[0027] At least one cyclically movable ring structure, a plurality of reaction cup positions for carrying reaction vessels are arranged at intervals on the ring structure, and a plurality of reaction cup positions for carrying out liquid suction operation or liquid injection operation are distributed along the circular movement path of the ring structure In this embodiment, the ring structure is a structure that is connected end to end and can be cyclically moved, and its shape is not limited. For example, the ring structure can be ...

Embodiment 2

[0039] For a better understanding of the present invention, the case where there are three annular structures is described here as Embodiment 2, and the situation of the remaining number of annular structures is similar. According to one embodiment of the present invention, a nucleic acid extraction device is provided, including: an incubation mechanism, a separation mechanism, an elution mechanism, a liquid suction mechanism, a liquid injection mechanism, and at least one transfer mechanism for transferring reaction containers into and out of each mechanism. It should be understood that here the nucleic acid extraction and detection are separated, and the nucleic acid extraction is described according to the three test steps of incubation, separation, and elution. In another example, the nucleic acid extraction may not be eluted, and the incubation, separation, and After the operation, the detection reagent can also be directly injected for detection.

[0040] The incubating ...

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Abstract

Provided are nucleic acid extraction apparatuses and operation methods thereof. The apparatus may include at least one cyclically moveable annular structure, at least one pipetting mechanism, at least one injection mechanism and a driving mechanism. The annular structure may be provided with a plurality of cuvette positions and a plurality of operation positions. The pipetting mechanism and the injection operation may be arranged along the annular structure. The driving mechanism may drive the annular structure to move cyclically.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction device and a working method thereof. Background technique [0002] Nucleic acid is the basic object of molecular biology research, and the nucleic acid extraction step is the most important and basic link in molecular diagnosis. There are many methods to extract DNA or RNA from biological samples, such as traditional organic solvent extraction method, silica gel molded column adsorption method, magnetic bead separation method and charge method, etc. Among them, the magnetic bead separation method is currently the most widely used method with the most mature automation conditions. The basic principle is to use the surface of small particles with magnetic materials to make them adsorb the required substances through certain treatments, and then enrich them by magnet adsorption. Finally, the waste liquid is discarded, and the enriched magne...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/00
CPCC12N15/1006G01N35/025G01N35/04G01N35/1002G01N2035/00534G01N2035/0444C12Q1/6848G01N35/0092G01N2035/00564G01N2035/0437
Inventor 王海翁彦雯冷彦宁解传芬
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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