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New diagnostic assay using particles with magnetic properties

A technology of magnetic particles and particles, applied in the direction of measuring devices, other chemical processes, separation methods, etc.

Inactive Publication Date: 2016-09-28
LAB ON A BEAD AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One problem is the control of particle movement

Method used

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  • New diagnostic assay using particles with magnetic properties
  • New diagnostic assay using particles with magnetic properties
  • New diagnostic assay using particles with magnetic properties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Example 1. Preparation of epoxide-activated agarose particles

[0129] Sepharose 4B (GE Healthcare Life Sciences) was washed with distilled water on a glass filter and blotted dry. 3 g of xerogel particles were suspended in 2.4 ml of 1M sodium hydroxide solution, and 0.45 ml of epichlorohydrin was added dropwise with stirring at room temperature. The temperature was raised to 60°C and maintained for 2 hours. The epoxide-activated agarose gel was washed neutral with distilled water on a glass filter and resuspended in distilled water at a final 50% gel concentration. This product constitutes an example of a porous particle as defined herein, see for example, figure 1 and figure 2 Particles in Pp.

Embodiment 2

[0130] Example 2. Production of agarose particles modified with magnetic particles comprising magnetic particles of one size.

[0131] Amino (NH 2 ) functionality of commercially available magnetic microparticles as magnetic particles. These particles are examples of magnetic particles as defined herein, see, for example, figure 1 and figure 2 Magnetic particles in Mp.

[0132] Make the magnetic particles as follows M-NH 2 (2 μm and 10 μm) were covalently attached to porous epoxy-activated agarose particles, respectively. will be from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Epoxide-activated agarose particles, i.e., 1 ml of settled gel, were resuspended in 10 ml of 0.01 M NaOH solution, added to the magnetic particle suspension, and reacted at room temperature under vortexing, for 2 μm particles 12 hours, and 30 minutes for 10 μ...

Embodiment 3

[0134] Example 3. Production of agarose particles modified with magnetic particles comprising magnetic particles of two sizes

[0135] Make the magnetic particles as follows M-NH 2 (5 μm and 10 μm) covalently attached to porous epoxy-activated agarose particles:

[0136] First, 10 μm from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Epoxide-activated agarose particles, that is, 1 ml of the settled gel, were resuspended in 10 ml of 0.01 M NaOH solution, added to the 10 μm magnetic particle suspension, and reacted for 30 minutes at room temperature under vortex shaking.

[0137] Second, 5 μm from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Then 5 μm magnetic particles were added to the agarose 10 μm magneti...

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PUM

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Abstract

A particles comprise non-magnetic porous particles, comprising at least one polymer, and having an exterior surface and an interior surface defined by said pores. The non-magnetic porous particle has functional groups on its inner and outer surface. A magnetic particle, which also has functional groups bonded to its surface, is covalently bonded to the non-magnetic porous particles by reacting the functional groups of the two particles and forming a covalent bond between them. The smallest average diameter of the magnetic particle is greater than the average diameter of the pores of the porous particle. In this way, the magnetic particle does not block the pores of the non-magnetic porous particle. The application also concerns a novel system and an assay for the analysis of molecules and cells with the magnetic particle. The magnetic particles on the non-magnetic particles form a characteristic pattern, fingerprint or bar code.

Description

technical field [0001] The present description generally relates to novel systems for the analysis of molecules and cells comprising clusters in which non-magnetic particles are supplemented with magnetic particles to form characteristic patterns or bar codes. Such particles and methods for their production are also disclosed. Background technique [0002] Techniques for analyzing biomolecules and cells in bodily fluids are critical in many technical fields such as, but not limited to, medicine, biopharmaceuticals, and biotechnology. Traditional immunological techniques such as enzyme-linked immunosorbent assay (ELISA) are still predominant. However, as the number of analyzes performed worldwide continues to increase, the need for faster and more efficient analytical methods becomes critical. Systems that rely on multiplex analysis represent one solution to a more efficient analytical system. Several commercial technologies using multiplex detection are now available. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281B01D15/38C08G81/00
CPCB01D15/3885B01D15/3804B01J20/285B01J20/286B01J20/3204B01J20/321B01J20/3212B01J20/3242B01J20/3265B01J20/28009B01J20/28016B01J20/24B01J20/3217C08B37/0039G01N33/5434G01N33/548
Inventor 斯文·奥斯卡尔森克里斯托弗·埃里克松彼得·斯韦德林德
Owner LAB ON A BEAD AB
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