Pharmaceutical composition of didanosine and application of pharmaceutical composition in biological medicines
A technology of didanosine and its composition, which is applied in the field of pharmaceutical composition and its application in biomedicine, and can solve the problems that have not yet been reported on the correlation between didanosine and its pharmaceutical composition and liver depression syndrome, To achieve the effect of improving the therapeutic effect
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[0017] Example 1: Compound (Ⅰ) isolation preparation and structure confirmation
[0018] Separation method: (a) Crush the small bark bone (3kg), heat reflux with 75% ethanol for extraction (20L×3 times), combine the extracts, concentrate until there is no alcohol smell (4L), and use petroleum ether (4L×3). Times), ethyl acetate (4L×3 times) and water-saturated n-butanol (4L×3 times) to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step ( a) The medium n-butanol extract is removed with AB-8 type macroporous resin, first eluted with 6% ethanol for 8 column volumes, and then eluted with 70% ethanol for 8 column volumes, collect 70% eluate, reduce Press and concentrate to obtain 70% ethanol elution concentrate; (c) In step (b), the 70% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 50:1 (8 column volumes), 25:1 (8 column volumes), 15:1 (8 column volumes) and 5:1 (10 column volumes) of dic...
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[0021] Example 2: Pharmacological effects
[0022] In this example, a rat model of liver depression syndrome was prepared by gavage of thyroxine tablets and fed with hunger and satiety. It was observed that the drug reduced animal plasma serotonin (5-hydroxytryptamine, 5-HT), norepinephrine (norepinephrine, NE), Epinephrine (epinephrine, E) level and other aspects of anti-liver depression syndrome.
[0023] 1. Materials and methods
[0024] 1.1 Animals
[0025] Adolescent healthy female SD rats, weighing (200±10) g, and the animal feed is ordinary solid feed, all provided by the Experimental Animal Center of Medical College of Xi’an Jiaotong University, and they are raised, observed and researched in the Physiology Laboratory of Medical College of Xi’an Jiaotong University. Laboratory temperature (20±3)℃, humidity (55±5)℃, 12h lighting and ventilation.
[0026] 1.2 Reagents and samples
[0027] Didanosine was purchased from the National Institute for the Control of Pharmaceutical and B...
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