Esophageal cancer cell line and application thereof

A technology for cancer cells and esophageal cancer, applied in the fields of biology and oncology, to achieve the effect of strong clone formation and tumorigenicity

Inactive Publication Date: 2016-10-12
ZHEJIANG CANCER HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a Chinese-derived esophageal cancer cell line for the lack of Chinese-derived esophageal cancer cell lines in China at present

Method used

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  • Esophageal cancer cell line and application thereof
  • Esophageal cancer cell line and application thereof
  • Esophageal cancer cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A tumor tissue sample was taken from a patient with esophageal cancer (the patient was a 54-year-old male, the tumor was located in the upper esophagus, postoperative pathology showed moderately differentiated squamous cell carcinoma, the TNM stage was T3N1M0, and the clinical stage was 3A.). After the separated fresh esophageal cancer tissue was washed with PBS in a sterile ultra-clean bench, it was cut into pieces and separated into 0.5-1mm pieces with ophthalmic forceps, scissors and other instruments in a petri dish. 3 Spread the small piece on the bottom of the dish, and add 10mL of 10% FBS (GIBCO company), 1% double antibody DMEM / F12 cell culture medium (GIBCO company) to the culture dish, put it in 37 ℃, 5% CO 2 cultured in an incubator. Change the medium after 5 to 7 days, discard the necrotic and floating small pieces in the tissue pieces, and carry out subculture. During the subculture process, the fibroblasts were continuously removed by taking advantage of ...

Embodiment 2

[0032] The subcultured human esophageal squamous cell line ZEC-014 cells were used to observe the growth of living cells under an optical microscope (Japan Olympus IMT-2 inverted microscope). Such as figure 1 As shown in the photo of the optical morphology of the cells, it can be seen that the cells grow vigorously, the background is clear, and impurities are rare. The cells are flat and irregular polygons.

Embodiment 3

[0034] Cell Index refers to the change of electrical impedance generated by the interaction between living cells and the microelectrode in the well of the detection plate. The xCELLigence Cell Function Analyzer converts these signals into specific parameters to become the Cell Index. The cell index is a good measure of the state of the cell - growth, proliferation, shape change, death, stress, etc. The cell index has been reviewed and adopted by many journals.

[0035]Human esophageal squamous cell carcinoma cell line ZEC-014 cells in good growth state were taken, digested with trypsin, made into cell suspension, and counted. Add culture medium to the E-Plate detection plate of the xCELLigence cell function analyzer and measure the background impedance value, then add 100 μL cell suspension (5000 cells) to the E-Plate detection plate, and place it in an ultra-clean bench at room temperature for 30 minutes. Put the E-Plate detection plate with added cells on the detection table...

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Abstract

The invention discloses an esophageal cancer cell line and application thereof. The esophageal cancer cell line is named a human esophageal squamous cancer cell line ZEC-014, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C201689. The human esophageal squamous cancer cell line ZEC-014 disclosed by the invention comes from tumor tissues after excision of an esophageal cancer patient in China, an STR (Specialized Technology Resources) detection result shows that the human esophageal squamous cancer cell line ZEC-014 is unique and is not in cross contamination with other cells during a primary culture process, the clone formation ability and the tumorigenicity are strong, and the human esophageal squamous cancer cell line ZEC-014 can be used as an ideal cell line for application in respects of esophageal cancer research, esophageal cancer detection kit development, drug screening and the like.

Description

technical field [0001] The invention relates to the technical fields of biology and oncology, in particular to an esophageal cancer cell line and its application. Background technique [0002] Esophageal cancer is a common malignant tumor of the digestive tract. About 300,000 people die from esophageal cancer every year in the world. Its morbidity and mortality vary widely from country to country. my country is one of the areas with a high incidence of esophageal cancer in the world, with an average of 150,000 deaths per year. There are more men than women, and the age of onset is more than 40 years old. The typical symptom of esophageal cancer is progressive dysphagia, first it is difficult to swallow dry food, then semi-liquid food, and finally water and saliva cannot be swallowed. In my country, the incidence of esophageal cancer ranks fifth in the whole body tumors, and its mortality rate ranks fourth. There are two main subtypes of esophageal cancer: squamous cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
CPCC12N5/0693A01K67/027A01K2227/105A01K2267/0331C12N2503/02G01N33/5011G01N33/5044
Inventor 苏丹熊磊毛伟敏蒋友华应莉莎谢正华吴军舟
Owner ZHEJIANG CANCER HOSPITAL
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