Porcine epidemic diarrhea virus local variant and application thereof
A technology for porcine epidemic diarrhea and mutant strains, which is applied in the direction of viruses, antiviral agents, and viral antigen components, and can solve the problem of uneven effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] Example 1: Isolation, purification and identification of porcine epidemic diarrhea virus clinical strain (PEDV ZJ15XS0101)
[0038] 1.1 Source of disease material
[0039] The disease material came from a pig farm in Xiaoshan, Zhejiang Province in January 2015. There were 1,300 sows. The 1-day-old suckling piglets showed vomiting, and then clinical symptoms such as diarrhea, dehydration, and death. Piglets younger than 7 days old The average morbidity rate was 48%, and the average mortality rate was 60%. Necropsy showed small intestine, mesentery and mesenteric lymph node bleeding, intestinal tube flatulence, thinning and other lesions. The small intestinal tissues of 1-day-old sick suckling piglets were collected for virus isolation.
[0040] 1.2 Virus isolation
[0041] After the filtered tissue suspension infected Vero cells, the first-generation virus appeared cell shrinkage, shedding, and syncytia at about 26 hours ( figure 1 ). With the increase of the number...
Embodiment 2
[0058] Example 2: Research on growth characteristics of PEDV ZJ15XS0101
[0059] 2.1 Effects of different harvesting times on PEDV titer and RNA copy number
[0060] After PEDV ZJ15XS0101P16 infected vero cells, the cytopathic changes at different infection times ( Figure 6 ) and virus titer and RNA copy number at harvest time ( Figure 7 ). The results showed that 10.5 hours after PEDV ZJ15XS0101P16 infection, Vero cells showed a large area of membrane fusion. At 14.5 hours, the cells were vacuolated, and at 19.5 hours, almost all the cells fell off. After testing, the virus liquid harvested at 14.5h after infection has a higher virus titer; compared with the virus titer of the harvested virus liquid at 10.5h, 19.5h and 23.5h, the average lg of the harvested virus liquid at 14.5h 10 TCID 50 were higher than 0.33, 0.32 and 0.49, but the difference was not significant (P>0.05) ( Figure 7 , A); PEDV RNA copy number was also high at 14.5h, compared with 23.5h, the differ...
Embodiment 3
[0065] Example 3: Study on the genetic stability of different generations of PEDV ZJ15XS0101
[0066] 3.1 Complete sequence analysis of different generations of PEDV ZJ15XS0101
[0067] PEDV ZJ15XS0101 was continuously passaged by vero cells, and the comparison results of the complete genome sequence of the 16th and 32nd passage viruses with those of the first passage are shown in Table 2. The results showed that the similarity of the nucleotide and amino acid sequences of the whole genomes of the three generations of viruses reached 99.99%.
[0068] Table 2 Nucleotide and amino acid differences of different generations of ZJ15XS0101
[0069]
[0070]
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More