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Docynia delavayi polyphenol extract and preparation method thereof

A technology of plant polyphenols and extracts, applied in plant raw materials, plant/algae/fungus/moss components, drug combinations, etc.

Inactive Publication Date: 2016-10-26
SICHUAN NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far there has been no match for Related reports on the maximum extraction and further separation and purification of polyphenols in genus plants

Method used

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  • Docynia delavayi polyphenol extract and preparation method thereof
  • Docynia delavayi polyphenol extract and preparation method thereof
  • Docynia delavayi polyphenol extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The present invention Preparation of plant polyphenol extracts

[0044] Accurately weigh the milled Docynia indica (Wall.) Dcne.leaves 1kg, ultrasonic power 800W, ultrasonic frequency 40KHz, with 63% ethanol according to the ratio of liquid to material 20:1 (mL / g), extract at 65°C for 41min, extract twice, and combine the extracts , concentrated to obtain crude extract;

[0045] Pretreatment of the D-101 macroporous adsorption resin: seal and soak the macroporous resin D-101 with 95% ethanol aqueous solution for 24 hours, rinse with ultrapure water until there is no alcohol smell; then use 5% HCl solution Seal and soak for 2-4 hours, rinse with ultra-pure water until neutral; finally seal and soak with 2% NaOH solution for 2-4 hours, rinse with ultra-pure water until neutral, and set aside;

[0046] The crude extract was taken and loaded on the pretreated D-101 macroporous adsorption resin, the mass concentration of the sample solution was 25mg / mL, the...

Embodiment 2

[0047] Embodiment 2 The present invention Preparation of plant polyphenol extracts

[0048] Accurately weigh the milled Docynia indica (Wall.) Dcne.leaves 1kg, ultrasonic power 800W, ultrasonic frequency 40KHz, with 63% ethanol according to the ratio of liquid to material 20:1 (mL / g), extract at 65°C for 41min, extract twice, and combine the extracts , concentrated to obtain crude extract;

[0049] Pretreatment of the D-101 macroporous adsorption resin: seal and soak the macroporous resin D-101 with 95% ethanol aqueous solution for 24 hours, rinse with ultrapure water until there is no alcohol smell; then use 5% HCl solution Seal and soak for 2-4 hours, rinse with ultra-pure water until neutral; finally seal and soak with 2% NaOH solution for 2-4 hours, rinse with ultra-pure water until neutral, and set aside;

[0050] The crude extract was taken and loaded on the pretreated D-101 macroporous adsorption resin, the mass concentration of the sample solution was 25mg / mL, the...

experiment example 1

[0052] The various factors of experimental example 1 ultrasonic extraction process Effect of polyphenol extraction rate

[0053] 1. Materials, reagents and instruments

[0054] Materials and Reagents

[0055] 栘 Docynia indica (Wall.) Dcne. leaves were collected in Xichang City, Sichuan Province in April 2014, and were identified by Professor Luo Qiang of Xichang University, which conformed to the characteristics of this species.

[0056] Phlorizin reference substance (HPLC > 98%, purchased from Chengdu Zhibiao Chemical Purification Co., Ltd., Sichuan Province); Folin-Ciocaileus reagent (FC reagent, purchased from Shanghai Ruji Biotechnology Co., Ltd.); ethanol (analytical grade), Anhydrous sodium carbonate (analytical pure) (purchased from Chengdu Kelong Co., Ltd.).

[0057] instrument

[0058] equipment name

model or specification

Manufacturer

UV spectrophotometer

UV-1700

Shimadzu

Vacuum freeze drying oven

wizard 2.0

Am...

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Abstract

The invention discloses a Docynia delavayi polyphenol extract which has polyphenol content of 91.24 to 91.34% (w / w). The invention also provides a preparation method for the extract. With extraction conditions provided in the invention, polyphenol substances can be maximally extracted from Docynia delavayi, and an extraction rate is as high as 18.54%. High-purity polyphenol and phlorizin can be obtained by further purifying a crude extract with the purifying method provided by the invention. Pharmacological experiments show that the Docynia delavayi polyphenol extract can substantially reduce the levels of cholesterol TC, triglyceride TG, high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in the serum of mice; thus, the Docynia delavayi polyphenol extract can effectively prevent and treat diseases related to abnormal lipids metabolism, such as obesity resulting from abnormal blood fat content, hyperlipidemia, atherosclerosis and coronary heart diseases.

Description

technical field [0001] The present invention relates to a kind of The invention belongs to a plant polyphenol extract and a preparation method thereof, and belongs to the fields of functional food and medicine. Background technique [0002] 栘 Rosaceae Generic name of plants in the genus, there are three species: Yunnan Docynia delavayi (Franch.) Schneid、栘 Docynia indica(Wall.)Dcne. and Longclaw Docynia longiunguisQ.Luo et J.L.Liu, mainly distributed in Yunnan, Guizhou, Sichuan and other places, among which Yunnan It is a famous local wild fruit. [0003] Currently, for the The research and development of the genus is still in its infancy, about the The basic research on the genus plants is still very insufficient, and the research on its active ingredients is very little, resulting in a large waste of resources. Liu Haixia et al. improved the traditional Folin-Ciocalteu colorimetry and established a highly specific colorimetry Determination method of poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/06A61P3/04A61P9/10
CPCA61K36/73A61K2236/333A61K2236/39A61K2236/55
Inventor 张晓喻刘刚王战国严春新任兰杨军
Owner SICHUAN NORMAL UNIVERSITY
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