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Molecular markers for five important pathogens and application thereof

A technology of molecular markers and pathogens, applied in the fields of biotechnology and genetics, can solve the problems of unfavorable early detection of diseases, cumbersome operations, and time-consuming

Inactive Publication Date: 2016-10-26
SHANGHAI QY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, clinical detection methods for these pathogenic bacteria are different; the general inspection procedures include four-step tests such as microscopy, culture, bacterial lysis test, and animal experiments. The operation is cumbersome and time-consuming, which is not conducive to the early detection of the disease

Method used

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  • Molecular markers for five important pathogens and application thereof
  • Molecular markers for five important pathogens and application thereof
  • Molecular markers for five important pathogens and application thereof

Examples

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example

[0035] Example: Utilize the method of the present invention to quickly identify whether the target individual is carrying a pathogen

[0036] (1) Collect serum samples from migrant workers returning from Africa, and extract individual genomic DNA. The reagent used to extract high-quality genomic DNA is the DNeasy Blood & Tissue Kit produced by QIAGEN (Germany). The specific method is as follows:

[0037] Put the individual serum into a 1.5ml EP tube, add 500 µl of 1.0 mM EDTA and 10mM Tris buffer. After oscillating, suck up the liquid and repeat three times. Add 180µl of ATL buffer provided in the kit; add 20µl proteinase K, and shake to mix. Incubate in a 56°C water bath for 4 h, shaking several times in between. Shake for 15 seconds after taking it out of the water bath, add 200 µl buffer AL, and shake to mix. Then add 200µl of 95% ethanol and mix well. Transfer all the liquid into the DNeasy extraction column, centrifuge at 8000g for 1min, and discard the filtrate. A...

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Abstract

The invention discloses a method for using nucleotide DNA fragments as molecular markers for identification of five important pathogens, i.e., Yersinia pestis, Plasmodium falciparum, Vibrio cholera, Leishmania donovani and Trypanosoma brucei. The nucleotide DNA fragments can be used as effective genetic markers for rapid and accurate identification of pathogens, are beneficial for accurate and rapid discrimination of populations migrated from an original infection site and provide bases for molecular epidemiological study of pathogens.

Description

technical field [0001] The invention belongs to the field of biotechnology and genetics, in particular, the invention relates to an application in molecular epidemiology of a molecular marker using a nucleotide DNA fragment as a molecular marker to identify five important pathogens. Background technique [0002] Plague, cholera, kala-azar, malaria, and sleeping sickness were notable epidemics, common in Africa. Sleeping sickness is at risk of infection to 70 million people in 36 countries in Africa; in modern times, there have been three outbreaks. Malaria was in 2012, with an estimated 207 million malaria cases and approximately 627,000 malaria deaths. Globally, an estimated 3.4 billion people are still at risk of malaria, mostly in Africa and South-East Asia. About 80% of malaria cases occur in Africa. Plague is a severe infectious disease. It has broken out many times in history and claimed the lives of tens of millions of people. Therefore, it has always been the focu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/689C12Q1/6893C12Q2600/158Y02A50/30
Inventor 刘骁胡薇杨忠殷明波
Owner SHANGHAI QY BIOTECH CO LTD
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