38 results about "Infections site" patented technology

The invention discloses a neuraminidase-targeted drug-loaded nanoparticle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. Polysialic acid and poly (lactic-co-glycolic acid) are connected through ester bond connection to obtain the drug-loaded nanoparticles with stable structure and performance, and the drug-loaded nanoparticles have the effect of targeting neuraminidase and also have high efficiency of entering cells; the antibiotic drug is further dispersed in the drug-loaded nanoparticles, the inflammation regulating agent is adsorbed on the surface of the drug-loaded nanoparticles, and the drug-loaded nanoparticles can deliver the antibiotic drug and the inflammation regulating agent to the pig Gleisseria in the pig body. According to the present invention, the antibiotic drug and the inflammation regulating agent are released under the acidic environment at the infection site in the pig body, such that the pig Gleisseria is efficiently killed, the inflammation damage caused by bacterial infection and the toxic damage of the antibiotic drug are reduced, and the good application prospect is provided in the preparation of the drug for preventing and / or treating the pig Gleisseria infection.

The invention discloses an antibacterial drug delivery system based on a dynamic response type microneedle patch and a preparation method, and belongs to the field of biomedical materials.The antibacterial drug delivery system comprises a substrate layer and a needle point layer arranged on the surface of the substrate layer, the substrate layer is a mixed matrix of polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), and the needle point layer is made of nano-silver; the needle tip layer is a phenylboronic acid modified oxidized hyaluronic acid and polyvinyl alcohol composite system. The antibacterial drug delivery system based on the dynamic response type microneedle patch, namely the microneedle patch with the pH response drug release function, is suitable for antibacterial treatment of infectious wounds, the mechanical strength of the needle point layer is improved, it can be better guaranteed that a microneedle completely penetrates into the skin, and the delivery efficiency is improved; meanwhile, drug release can be responded according to the pH of a bacterial infection part, and the antibacterial effect is good.

The invention relates to a nano-drug delivery system targeting neutrophil and a preparation method and application thereof. The nano-drug delivery system is prepared by the following method: S1, preparing a liposome 1 wrapping a drug 1; s2, preparing a liposome 2 wrapping a medicine 2; and S3, mixing and incubating the liposome 1 wrapping the medicine 1 and the liposome 2 wrapping the medicine 2, and connecting the corresponding ligand and receptor in the liposome 1 and the liposome 2 to obtain the liposome. The ligand and the receptor are respectively biotin and streptavidin. The nano-drug delivery system is obtained by successfully connecting two drug liposomes with different functions through a ligand and a receptor system, so that the chemotaxis of neutrophil to an infected part can be enhanced, the antibacterial property of NETs can be improved, and the nano-drug delivery system has a very good anti-infection effect.

A hydrogel composition for intranasal delivery of an antifungal (amphotericin B) at an infection site, specifically the nasal mucosa and upper respiratory tract.

The preparation method comprises the following steps: grinding an iodine block to obtain iodine powder, adding the iodine powder into double distilled water, and carrying out ultrasonic stripping to obtain an iodine nanosheet suspension; under the conditions of stirring and water bath heating, dissolving polyvinyl alcohol with double distilled water to obtain a polyvinyl alcohol solution, adding sodium alginate in batches, and continuously stirring in a water bath to obtain a moldable composite hydrogel precursor solution; dissolving tannic acid by adopting an iodine nanosheet suspension, then adding into the moldable composite hydrogel precursor solution, uniformly stirring at room temperature, adjusting the pH value of the solution to be neutral, finally dropwise adding a borax solution, and stirring into gel to obtain the I2 / PBST moldable antibacterial composite hydrogel. The prepared composite hydrogel not only can play an antibacterial role and reduce the bacterial infection probability of infection sites, but also has excellent properties such as plasticity, stretchability, self-healing property, adhesion and degradability, is convenient to fill defect parts with different shapes, and is firm in adhesion.

Compositions of chitosan and one or more polyalcohols, such as mannitol and polyethylene glycol, and methods of use thereof, are provided. The disclosed compositions can deliver high concentrations of localized antimicrobials and metabolites to make infections more susceptible to antimicrobials. The disclosed compositions may be used to deliver biologically active agents to an infection at a site of trauma or surrounding an indwelling medical device, to treat an infection caused by persister bacterial cells, and to treat and / or prevent the formation of biofilm bacteria on an indwelling medical device or surrounding tissue.

Provided are compounds, compositions, and methods for treatment of fungal infections. The compositions comprise the mannose disaccharide analog (2R,3S,4S,5S,6R)-2- (((((3S,4R,5R)-4,5-dihydroxypiperidin-3-yl)methyl)thio)-6-(hydroxymethyl)tetrahydro-2H- pyran-3,4,5-triol. Methods are also provided for the treatment of fungal infections comprising contacting the infected site with a therapeutically effective concentration of the present compound or compositions. [Image disponible dans le document PDF, Image available in the PDF document]

This invention relates to the field of bacterial infection treatment technology, and discloses an enzyme-responsive probe, its preparation method, and its applications. The probe of this invention constructs an enzyme-responsive "prodrug" structure by introducing cephalosporin derivative linker units that can be specifically cleaved by β-lactamases onto a hemicyanine optical framework. This structure remains optically and therapeutically inert in normal tissues, but is selectively activated at the site of infection by drug-resistant bacteria. When the probe contacts multidrug-resistant bacteria that overexpress β-lactamases, the linker units undergo enzymatic cleavage, releasing hemicyanine active molecules with near-infrared fluorescence emission and highly efficient photothermal conversion capabilities, achieving high-contrast fluorescence imaging and controllable photothermal sterilization of the infection focus. The probe of this invention has a simple synthetic route, mild conditions, and reliable purification methods, making it suitable for large-scale preparation, especially for the precise diagnosis and targeted photothermal synergistic treatment of superficial tissue infections such as bacterial keratitis.

The present application provides a polymyxin prodrug compound with the structure shown in formula (I) or a stereoisomer or a pharmaceutically acceptable salt thereof. The polymyxin prodrug compound of the present application can effectively reduce the biological toxicity of polymyxin, selectively activate in bacterial infected tissues, achieve bacterial killing in infected sites, improve the bioavailability of polymyxin, achieve sustainable development and utilization of classic antibiotics, expand the scope of future clinical application of polymyxin, and has a broad clinical application prospect.

The invention belongs to the technical field of radiopharmaceutical chemistry and clinical nuclear medicine, and particularly relates to an antibacterial peptide UBI 29-41 derivative containing proline as well as a preparation method and application thereof. The proline-containing antibacterial peptide UBI 29-41 derivative is labeled by radionuclide to obtain a radioactive preparation, and the radioactive preparation has high uptake in a bacterial infection part, has a good infection / non-target ratio, and can be specifically combined with bacteria. The radioactive preparation provided by the invention can be used as a novel radioactive drug for diagnosis of bacterial infection, and has good clinical popularization and application prospects and value.

The invention discloses an engineered polypeptide injection system and application thereof in targeted delivery of antibacterial peptides, and belongs to the technical field of biology. The engineered polypeptide injection system provided by the invention can accurately deliver the target polypeptide to a targeted infection site, reduce whole body exposure, reduce toxicity and give full play to the effect of the target polypeptide. The invention also provides application of the engineered polypeptide injection system in preparation of a product for eliminating mycobacteria in target cells and preparation of a product for preventing and / or treating lung diseases and / or symptoms caused by the mycobacteria, and target polypeptides Granulysin, HNP1, and LysA and / or LysB derived from mycobacteriophage D29 or MS6 are delivered to macrophages, so that the lung diseases and / or symptoms caused by the mycobacteria are prevented and treated, and the lung diseases and / or symptoms caused by the mycobacteria are prevented and treated. The mycobacteria can be effectively removed.

The invention discloses a modularized pathogenic microorganism targeting fluorescent probe, an antibacterial drug delivery oligopeptide and a preparation method and application thereof, the probe is composed of the following functional modules: a chemotactic module: formyl-D-Phe-Leu-D-Met, which realizes active migration to an infectious inflammation microenvironment by combining with a formyl peptide receptor; the targeting module comprises D-Trp-D-Trp-Arg and RWWR which are specifically combined with the surfaces of the pathogenic microorganisms through cationic interaction and hydrophobic interaction; the fluorescence activation module is a double Cy5.5 fluorophore which is covalently connected through lysine side chain amino; and the connection and stability module is a PEG4-GGSG flexible linker, has C-terminal amidation and key site D-type amino acid modification, and is used for enhancing the stability of the probe. The probe can actively migrate to an infectious inflammation microenvironment, is specifically combined with the surface of pathogenic microorganisms, and triggers fluorescence activation after combination, so that accurate imaging of an infected part is realized.

Provided herein are rapid and effective local infection therapy methods, systems, and devices that significantly reduce the mortality, morbidity, and the cost of care in rare musculoskeletal infections. Continuous delivery of antibiotic therapy locally, at the infection site, reduces edema and provides antibiotic irrigation, significantly improving outcomes while reducing the need for systemic antibiotics.

A method for delivery of an active antibiotic agent to a localized, infected site involves steps of creating an ointment by combining an active antibiotic agent with a tissue penetrating solvent suitable for solubilizing a non-liquid active pharmaceutical ingredient, a tissue penetrating diluent, and a stabilizer for maintaining the ointment, which also functions to as a stabilizer for maintaining chemical stability and resistance to degradation, adjusting viscosity of the ointment by adjustment of amount of the solvent, determining location and extent of the infected site, and applying a predetermined volume of the ointment over skin at the infected region.

The invention discloses a hybrid membrane coated BCZT nano material as well as a preparation method and application thereof, and belongs to the technical field of biomedical treatment. The preparation method of the hybrid membrane coated BCZT nano material comprises the following steps: preparing a BCZT piezoelectric nano material; preparing hybrid membrane vesicles; and uniformly coating the hybrid membrane vesicles on the surface of the BCZT piezoelectric nano material by using a liposome extruder and an ultrasonic dispersion instrument to obtain the hybrid membrane coated BCZT nano material. The BCZT nano material disclosed by the invention can efficiently generate reactive oxygen species (ROS) under ultrasonic catalysis, has a killing effect on bacteria, has very strong tissue penetrability, and can go deep into infected parts to realize an effective antibacterial effect. And finally, the BCZT material is wrapped by a hybrid membrane wrapping technology, so that the stability and biocompatibility of the nano material can be improved, the nano material is more stable in an in-vivo environment, and possible side effects are reduced.

This invention relates to the field of biomedical technology, specifically to an antifungal nanoparticle, a combination drug, and its application. The antifungal nanoparticle is prepared by covalently linking the aptamer AU1 to drug-loaded silk fibroin nanoparticles via amide bonds. The mass ratio of the drug-loaded silk fibroin nanoparticles to the aptamer AU1 is 20:0.5~2.0. The antifungal nanoparticles of this invention are prepared by a desolvation method using voriconazole-loaded silk fibroin nanoparticles, and the targeting aptamer AU1 is attached to their surface using a carbodiimide chemical cross-linking method. This allows for specific recognition of Candida albicans, increasing drug accumulation at the infection site. Further combination with methylene blue-mediated photodynamic therapy can disrupt biofilm structures, enhance nanoparticle penetration, achieve synergistic bactericidal activity, and reduce systemic toxicity, providing a novel, highly effective, and low-toxicity treatment strategy for fungal biofilm infections.

A method of treating microbial infections that entails producing a space over the infected site by covering such site with an impermeable material and then filling such space with an antimicrobial material (in the form of a solution, cream, gel, colloid, etc) comprising an efflux pump inhibitor soluble in a non-polar solvent is provided. The antimicrobial material can also be applied in multiple other ways such as a cream or released from a band-aid or gauze. Such treatment method may also be used with newly discovered antibiotics.

The invention belongs to the technical field of biological medicine, discloses nanocapsule-loaded in-situ gel for treating H. pylori infection and reversing intestinal metaplasia of gastric tissue and application of the nanocapsule-loaded in-situ gel, and particularly discloses nanocapsule-loaded in-situ gel which comprises a nanocapsule and pH-responsive gel, the nanocapsule is dispersed in the gel, and the pH-responsive gel is a pH-responsive gel. Comprising an active drug, a macromolecular substance for coating the active drug and a structure stabilizer. According to the nanocapsule-loaded in-situ gel provided by the invention, the nanocapsule-loaded in-situ gel is directionally released on one side by responding to the change of the pH value of an H.pylori infected part, and the nanocapsule responds to lipase secreted by H.pylori to accurately release an active drug, so that efficient sterilization is realized, and damage caused by infection is relieved. Meanwhile, the gel can also effectively reverse gastrointestinal metaplasia caused by H.pylori infection, further reduces the risk of gastric cancer, and provides a new thought for gastric cancer prevention.

The invention discloses pH / neuraminidase dual-response drug-loaded nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The drug-loaded nanoparticle comprises polysialic acid and amino acid, wherein carboxyl on a side chain of the polysialic acid is connected with amino in the amino acid through an amido bond; polysialic acid and amino acid are connected through an amido bond to obtain the drug-loaded nanoparticles with stable structure and performance, and the drug-loaded nanoparticles have the effect of targeting neuraminidase, and also have low cytotoxicity and high efficiency of entering cells; according to the present invention, after the antibiotic drug is connected to the drug-loaded nanoparticle through the ester bond, the drug-loaded nanoparticle can deliver the antibiotic drug to the pig Gleisseria in the pig body, and the antibiotic drug is released in the acidic environment at the infection site in the pig body so as to efficiently kill the pig Gleisseria, such that the drug-loaded nanoparticle can be used for preparing the drug-loaded nanoparticle, the compound has a good application prospect in preparation of drugs for preventing and / or treating swine Gleisseria infection.

The invention provides an infectious fever antibacterial scheme selection method and system based on multi-dimensional data, and belongs to the technical field of infectious disease diagnosis and treatment. The method comprises the following steps: step (1), identifying a fever type based on symptom and sign data and inspection data, and positioning a pathogen type, an infection site and severity; (2) generating candidate antibiotic schemes based on the antibiotic knowledge base and a guide rule engine; (3) adjusting the dosage according to the individual parameters of the patient, and generating a first scheme and an alternative scheme; through multi-dimensional data integration, infection types are rapidly identified, antibiotics recommended by a guide are matched, and an accurate and safe antibacterial scheme (including a first scheme and an alternative scheme) is generated according to individual conditions of patients.

To provide a method for treating implantable device infections. [Solution] The present invention relates to the field of phage therapy, and more particularly to providing phage-based compositions and methods for treating or preventing infections associated with implantable devices. The compositions may be administered directly to the infection site of the device, as a single dose and / or by other means as needed, potentially avoiding the need to replace the implantable device. The method may include (a) obtaining a biological sample from the subject; (b) culturing bacteria present in the biological sample; and (c) inoculating the cultured bacteria with a phage to lyse them.

The application provides a pH-responsive disintegrating amphiphilic dextran block polymer, a cationic antibacterial peptide nanodrug and application, utilizes the pH-responsive disintegrating amphiphilic dextran block polymer and the nanometer AMPs to utilize the electrostatic force, the N-B coordination bond force and the guanyl-π electron force to self-assemble into the cationic antibacterial peptide nanodrug, realizes the effective capture of the nanometer AMPs, not only improves the drug loading of the amphiphilic dextran block polymer, but also can protect the free amino group in the nanometer AMPs residue, improves the stability of the nanometer AMPs to protease, reduces the toxicity to normal cells and reduces the risk of hemolysis; and the cationic antibacterial peptide nanodrug gradually hydrolyzes under the weak acid environment of the infection site, so that the drug is enriched at the infection site and the bioavailability is improved, and a new solution is provided for overcoming the increasingly serious drug-resistant bacterial infection problem in clinic.

The present invention discloses a covalent organic framework antibacterial material based on o-phenanthroline and its application, belonging to the field of biomedicine technology. The covalent organic framework antibacterial material is obtained by the coordination reaction of 5,5'-[(1E,1'E)-({1,10-phenanthroline-5,6-diyl}bis(azeninyl))bis(methylene)]bis(1,2,4-benzenetriol) PRSA with copper to obtain a covalent organic framework antibacterial material PRSA-Cu based on o-phenanthroline. The PRSA-Cu prepared by the present invention can utilize photothermal capacity to combine simulated photothermal (PTT), photodynamic therapy (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to achieve synergistic antibacterial effects. It can also utilize the inherent characteristics of the infection microenvironment to induce bacterial death and significantly accelerate the recovery of bacterial-infected wounds by regulating the microenvironment of the bacterial infection site.

The invention discloses a cationic polymer antibiotic delivery carrier and a preparation method and application thereof, the structure of the cationic polymer antibiotic delivery carrier is as shown in the following formula (I): # imgabs0 #, R is beta-cyclodextrin or gamma-cyclodextrin; r1 is H, beta-cyclodextrin or gamma-cyclodextrin; m and n are positive integers, and are 100gt; m + ngt; 10. The epsilon-PLL-CD carrier disclosed by the invention can be rapidly enriched, permeated and uniformly distributed on a biological membrane of an infected part through reversible electrostatic interaction with an electronegative biological membrane, and meanwhile, the epsilon-PLL-CD carrier is combined with antibiotic molecules through supramolecular interaction between cyclodextrin and antibiotics, so that the antibiotic molecules are helped to be efficiently enriched on the infected part of the biological membrane; the permeation barrier effect of a biological membrane on antibiotics is overcome, and the efficient anti-biological membrane infection removal efficiency is achieved.

The invention discloses an anti-blocking closed flushing drainage tube for treating spinal deep infection, and relates to the technical field of drainage tubes. The anti-blocking closed flushing drainage tube for treating spinal deep infection comprises a drainage tube body, one end of the drainage tube body is fixedly connected with an annular cover, a plurality of flushing holes arranged in an array mode are formed in the side wall of the annular cover, an insertion hole is formed in one end of the annular cover, a flushing tube is inserted into the insertion hole, and the annular cover is fixedly connected with the drainage tube body. And the other end of the flushing tube penetrates through the side wall of the drainage tube body. According to the anti-blocking closed flushing drainage tube for treating spinal deep infection, flushing and drainage can be combined, use is more convenient, and the effect is better; during drainage, an infected part can be cleaned, soft tissue and the like adhered to the infected part can be conveniently separated, meanwhile, large soft tissue and the like can be extruded, crushed and cut up, blockage of the drainage tube body is avoided, the flushing and drainage efficiency is higher, and the flushing and drainage effect is better.

The present invention provides a co-loaded bifunctional nanogel of an antimicrobial peptide and a prebiotic and its application. First, the present invention realizes the encapsulation of the prebiotic by the secondary desolvation method, and then realizes the encapsulation of the antimicrobial peptide by the swelling method to obtain the co-loaded bifunctional nanogel of the antimicrobial peptide and the prebiotic. The preparation method of the present invention is simple and easy to implement and is easy to promote. The nanogel prepared by the present invention has the characteristics of bacterial specificity and time-controlled release, effectively exerting the biological effects of both. It can not only first locally slowly release OH-CATH 30 to kill pathogenic bacteria such as Staphylococcus aureus, and secondly, the inulin slowly released subsequently can promote the growth of probiotics in the vagina to a certain extent. The nanogel of the present invention can release the antimicrobial peptide and the prebiotic successively, thereby effectively killing pathogenic bacteria at the infection site and promoting the growth of probiotics, and can be applied to the preparation of a biodegradable preparation for anti-bacterial infection and promoting the rapid recovery of the lactic acid bacteria microecology.

The invention belongs to the technical field of nano-drugs, and particularly relates to multifunctional honokiol nano-particles and a preparation method and application thereof.The honokiol nano-particles are prepared by using rhamnolipid modified chitosan as a carrier and loading honokiol; according to the nano-particles, an amido bond formed between rhamnolipid and chitosan can be subjected to targeted decomposition under the acidic condition of a bacterial infection part, and the released rhamnolipid can destroy a bacterial biofilm; further, permeation of the natural antibacterial agent honokiol and chitosan at the infected part is enhanced, and synergistic sterilization is realized; meanwhile, the planktonic bacteria re-adhesion resistance characteristic of rhamnolipid can inhibit the re-formation of a biofilm, the full-stage sterilization of'destroying the biofilm, killing bacteria in the film and inhibiting the re-adhesion of residual bacteria 'of the nanoparticles is finally realized, and the multifunctional honokiol nano platform is suitable for preventing and treating infectious diseases such as wound infection and bacterial peritonitis.

Provided herein are rapid and effective local infection therapy methods, systems, and devices that significantly reduce the mortality, morbidity, and the cost of care in rare musculoskeletal infections. Continuous delivery of antibiotic therapy locally, at the infection site, reduces edema and provides antibiotic irrigation, significantly improving outcomes while reducing the need for systemic antibiotics.

The invention discloses an antibacterial polypeptide high polymer material, a product containing the antibacterial polypeptide high polymer material, and a preparation method and application of the antibacterial polypeptide high polymer material, and belongs to the technical fields of high polymer materials, bacterial infection treatment and medicines. The preparation method of the antibacterial polypeptide high polymer material comprises the following steps: carrying out esterification reaction on enol and glutamic acid, and carrying out glutamic acid side group modification to obtain an R2-NCA monomer; taking R1-NH2 as an initiator, and reacting with the R2-NCA monomer to obtain polypeptide; primary amine or quaternary amine is modified to a polypeptide side group through click reaction of sulfydryl and double bonds, and finally the antibacterial polypeptide high polymer material is prepared. The antibacterial polypeptide high polymer material with a specific structure prepared by the method can effectively penetrate through and resist a biological membrane matrix barrier and specifically target bacterial membrane phospholipids; the compound shows excellent in-vivo antibacterial activity, can effectively inhibit biofilm-related infection and promote repair of infected parts, and has good treatment effect and application prospect.

The utility model discloses a lavage device for an orthopedic postoperative infected part, and relates to the technical field of lavage devices. The orthopedic postoperative infected part lavage device comprises a box body, a disinfection mechanism used for disinfection and sterilization is fixedly installed on one side of the top of the box body, and a safety protection mechanism is fixedly installed on one side of the bottom in the box body; the disinfection mechanism comprises a mounting base, a constant-speed motor used for providing a power source is fixedly mounted on the front face of the mounting base, a connecting piece is fixedly mounted at the output end of the constant-speed motor, a shell is fixedly mounted at one end of the connecting piece, and multiple sets of ultraviolet lamps used for disinfection and sterilization are mounted in the shell at equal intervals. A controller is used for controlling a constant-speed motor to be electrified and run to drive a connecting piece to rotate in preset time, the rotating connecting piece can drive a shell to rotate to a designated position, and then an ultraviolet lamp is electrified and runs to emit ultraviolet light to disinfect and sterilize the contact surface of a spray pipe and a user; the function of intelligently disinfecting and sterilizing the device is realized.