24 results about "Infections site" patented technology

The invention discloses an antibacterial drug delivery system based on a dynamic response type microneedle patch and a preparation method, and belongs to the field of biomedical materials.The antibacterial drug delivery system comprises a substrate layer and a needle point layer arranged on the surface of the substrate layer, the substrate layer is a mixed matrix of polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), and the needle point layer is made of nano-silver; the needle tip layer is a phenylboronic acid modified oxidized hyaluronic acid and polyvinyl alcohol composite system. The antibacterial drug delivery system based on the dynamic response type microneedle patch, namely the microneedle patch with the pH response drug release function, is suitable for antibacterial treatment of infectious wounds, the mechanical strength of the needle point layer is improved, it can be better guaranteed that a microneedle completely penetrates into the skin, and the delivery efficiency is improved; meanwhile, drug release can be responded according to the pH of a bacterial infection part, and the antibacterial effect is good.

A hydrogel composition for intranasal delivery of an antifungal (amphotericin B) at an infection site, specifically the nasal mucosa and upper respiratory tract.

Compositions of chitosan and one or more polyalcohols, such as mannitol and polyethylene glycol, and methods of use thereof, are provided. The disclosed compositions can deliver high concentrations of localized antimicrobials and metabolites to make infections more susceptible to antimicrobials. The disclosed compositions may be used to deliver biologically active agents to an infection at a site of trauma or surrounding an indwelling medical device, to treat an infection caused by persister bacterial cells, and to treat and / or prevent the formation of biofilm bacteria on an indwelling medical device or surrounding tissue.

This invention relates to the field of bacterial infection treatment technology, and discloses an enzyme-responsive probe, its preparation method, and its applications. The probe of this invention constructs an enzyme-responsive "prodrug" structure by introducing cephalosporin derivative linker units that can be specifically cleaved by β-lactamases onto a hemicyanine optical framework. This structure remains optically and therapeutically inert in normal tissues, but is selectively activated at the site of infection by drug-resistant bacteria. When the probe contacts multidrug-resistant bacteria that overexpress β-lactamases, the linker units undergo enzymatic cleavage, releasing hemicyanine active molecules with near-infrared fluorescence emission and highly efficient photothermal conversion capabilities, achieving high-contrast fluorescence imaging and controllable photothermal sterilization of the infection focus. The probe of this invention has a simple synthetic route, mild conditions, and reliable purification methods, making it suitable for large-scale preparation, especially for the precise diagnosis and targeted photothermal synergistic treatment of superficial tissue infections such as bacterial keratitis.

The present application provides a polymyxin prodrug compound with the structure shown in formula (I) or a stereoisomer or a pharmaceutically acceptable salt thereof. The polymyxin prodrug compound of the present application can effectively reduce the biological toxicity of polymyxin, selectively activate in bacterial infected tissues, achieve bacterial killing in infected sites, improve the bioavailability of polymyxin, achieve sustainable development and utilization of classic antibiotics, expand the scope of future clinical application of polymyxin, and has a broad clinical application prospect.

The invention belongs to the technical field of radiopharmaceutical chemistry and clinical nuclear medicine, and particularly relates to an antibacterial peptide UBI 29-41 derivative containing proline as well as a preparation method and application thereof. The proline-containing antibacterial peptide UBI 29-41 derivative is labeled by radionuclide to obtain a radioactive preparation, and the radioactive preparation has high uptake in a bacterial infection part, has a good infection / non-target ratio, and can be specifically combined with bacteria. The radioactive preparation provided by the invention can be used as a novel radioactive drug for diagnosis of bacterial infection, and has good clinical popularization and application prospects and value.

The invention discloses an engineered polypeptide injection system and application thereof in targeted delivery of antibacterial peptides, and belongs to the technical field of biology. The engineered polypeptide injection system provided by the invention can accurately deliver the target polypeptide to a targeted infection site, reduce whole body exposure, reduce toxicity and give full play to the effect of the target polypeptide. The invention also provides application of the engineered polypeptide injection system in preparation of a product for eliminating mycobacteria in target cells and preparation of a product for preventing and / or treating lung diseases and / or symptoms caused by the mycobacteria, and target polypeptides Granulysin, HNP1, and LysA and / or LysB derived from mycobacteriophage D29 or MS6 are delivered to macrophages, so that the lung diseases and / or symptoms caused by the mycobacteria are prevented and treated, and the lung diseases and / or symptoms caused by the mycobacteria are prevented and treated. The mycobacteria can be effectively removed.

The invention discloses a modularized pathogenic microorganism targeting fluorescent probe, an antibacterial drug delivery oligopeptide and a preparation method and application thereof, the probe is composed of the following functional modules: a chemotactic module: formyl-D-Phe-Leu-D-Met, which realizes active migration to an infectious inflammation microenvironment by combining with a formyl peptide receptor; the targeting module comprises D-Trp-D-Trp-Arg and RWWR which are specifically combined with the surfaces of the pathogenic microorganisms through cationic interaction and hydrophobic interaction; the fluorescence activation module is a double Cy5.5 fluorophore which is covalently connected through lysine side chain amino; and the connection and stability module is a PEG4-GGSG flexible linker, has C-terminal amidation and key site D-type amino acid modification, and is used for enhancing the stability of the probe. The probe can actively migrate to an infectious inflammation microenvironment, is specifically combined with the surface of pathogenic microorganisms, and triggers fluorescence activation after combination, so that accurate imaging of an infected part is realized.

Provided herein are rapid and effective local infection therapy methods, systems, and devices that significantly reduce the mortality, morbidity, and the cost of care in rare musculoskeletal infections. Continuous delivery of antibiotic therapy locally, at the infection site, reduces edema and provides antibiotic irrigation, significantly improving outcomes while reducing the need for systemic antibiotics.

A method for delivery of an active antibiotic agent to a localized, infected site involves steps of creating an ointment by combining an active antibiotic agent with a tissue penetrating solvent suitable for solubilizing a non-liquid active pharmaceutical ingredient, a tissue penetrating diluent, and a stabilizer for maintaining the ointment, which also functions to as a stabilizer for maintaining chemical stability and resistance to degradation, adjusting viscosity of the ointment by adjustment of amount of the solvent, determining location and extent of the infected site, and applying a predetermined volume of the ointment over skin at the infected region.

This invention relates to the field of biomedical technology, specifically to an antifungal nanoparticle, a combination drug, and its application. The antifungal nanoparticle is prepared by covalently linking the aptamer AU1 to drug-loaded silk fibroin nanoparticles via amide bonds. The mass ratio of the drug-loaded silk fibroin nanoparticles to the aptamer AU1 is 20:0.5~2.0. The antifungal nanoparticles of this invention are prepared by a desolvation method using voriconazole-loaded silk fibroin nanoparticles, and the targeting aptamer AU1 is attached to their surface using a carbodiimide chemical cross-linking method. This allows for specific recognition of Candida albicans, increasing drug accumulation at the infection site. Further combination with methylene blue-mediated photodynamic therapy can disrupt biofilm structures, enhance nanoparticle penetration, achieve synergistic bactericidal activity, and reduce systemic toxicity, providing a novel, highly effective, and low-toxicity treatment strategy for fungal biofilm infections.

The invention belongs to the technical field of biological medicine, discloses nanocapsule-loaded in-situ gel for treating H. pylori infection and reversing intestinal metaplasia of gastric tissue and application of the nanocapsule-loaded in-situ gel, and particularly discloses nanocapsule-loaded in-situ gel which comprises a nanocapsule and pH-responsive gel, the nanocapsule is dispersed in the gel, and the pH-responsive gel is a pH-responsive gel. Comprising an active drug, a macromolecular substance for coating the active drug and a structure stabilizer. According to the nanocapsule-loaded in-situ gel provided by the invention, the nanocapsule-loaded in-situ gel is directionally released on one side by responding to the change of the pH value of an H.pylori infected part, and the nanocapsule responds to lipase secreted by H.pylori to accurately release an active drug, so that efficient sterilization is realized, and damage caused by infection is relieved. Meanwhile, the gel can also effectively reverse gastrointestinal metaplasia caused by H.pylori infection, further reduces the risk of gastric cancer, and provides a new thought for gastric cancer prevention.

The invention provides an infectious fever antibacterial scheme selection method and system based on multi-dimensional data, and belongs to the technical field of infectious disease diagnosis and treatment. The method comprises the following steps: step (1), identifying a fever type based on symptom and sign data and inspection data, and positioning a pathogen type, an infection site and severity; (2) generating candidate antibiotic schemes based on the antibiotic knowledge base and a guide rule engine; (3) adjusting the dosage according to the individual parameters of the patient, and generating a first scheme and an alternative scheme; through multi-dimensional data integration, infection types are rapidly identified, antibiotics recommended by a guide are matched, and an accurate and safe antibacterial scheme (including a first scheme and an alternative scheme) is generated according to individual conditions of patients.

To provide a method for treating implantable device infections. [Solution] The present invention relates to the field of phage therapy, and more particularly to providing phage-based compositions and methods for treating or preventing infections associated with implantable devices. The compositions may be administered directly to the infection site of the device, as a single dose and / or by other means as needed, potentially avoiding the need to replace the implantable device. The method may include (a) obtaining a biological sample from the subject; (b) culturing bacteria present in the biological sample; and (c) inoculating the cultured bacteria with a phage to lyse them.

The application provides a pH-responsive disintegrating amphiphilic dextran block polymer, a cationic antibacterial peptide nanodrug and application, utilizes the pH-responsive disintegrating amphiphilic dextran block polymer and the nanometer AMPs to utilize the electrostatic force, the N-B coordination bond force and the guanyl-π electron force to self-assemble into the cationic antibacterial peptide nanodrug, realizes the effective capture of the nanometer AMPs, not only improves the drug loading of the amphiphilic dextran block polymer, but also can protect the free amino group in the nanometer AMPs residue, improves the stability of the nanometer AMPs to protease, reduces the toxicity to normal cells and reduces the risk of hemolysis; and the cationic antibacterial peptide nanodrug gradually hydrolyzes under the weak acid environment of the infection site, so that the drug is enriched at the infection site and the bioavailability is improved, and a new solution is provided for overcoming the increasingly serious drug-resistant bacterial infection problem in clinic.

The present invention discloses a covalent organic framework antibacterial material based on o-phenanthroline and its application, belonging to the field of biomedicine technology. The covalent organic framework antibacterial material is obtained by the coordination reaction of 5,5'-[(1E,1'E)-({1,10-phenanthroline-5,6-diyl}bis(azeninyl))bis(methylene)]bis(1,2,4-benzenetriol) PRSA with copper to obtain a covalent organic framework antibacterial material PRSA-Cu based on o-phenanthroline. The PRSA-Cu prepared by the present invention can utilize photothermal capacity to combine simulated photothermal (PTT), photodynamic therapy (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to achieve synergistic antibacterial effects. It can also utilize the inherent characteristics of the infection microenvironment to induce bacterial death and significantly accelerate the recovery of bacterial-infected wounds by regulating the microenvironment of the bacterial infection site.

The invention belongs to the technical field of nano-drugs, and particularly relates to multifunctional honokiol nano-particles and a preparation method and application thereof.The honokiol nano-particles are prepared by using rhamnolipid modified chitosan as a carrier and loading honokiol; according to the nano-particles, an amido bond formed between rhamnolipid and chitosan can be subjected to targeted decomposition under the acidic condition of a bacterial infection part, and the released rhamnolipid can destroy a bacterial biofilm; further, permeation of the natural antibacterial agent honokiol and chitosan at the infected part is enhanced, and synergistic sterilization is realized; meanwhile, the planktonic bacteria re-adhesion resistance characteristic of rhamnolipid can inhibit the re-formation of a biofilm, the full-stage sterilization of'destroying the biofilm, killing bacteria in the film and inhibiting the re-adhesion of residual bacteria 'of the nanoparticles is finally realized, and the multifunctional honokiol nano platform is suitable for preventing and treating infectious diseases such as wound infection and bacterial peritonitis.

The invention discloses an antibacterial polypeptide high polymer material, a product containing the antibacterial polypeptide high polymer material, and a preparation method and application of the antibacterial polypeptide high polymer material, and belongs to the technical fields of high polymer materials, bacterial infection treatment and medicines. The preparation method of the antibacterial polypeptide high polymer material comprises the following steps: carrying out esterification reaction on enol and glutamic acid, and carrying out glutamic acid side group modification to obtain an R2-NCA monomer; taking R1-NH2 as an initiator, and reacting with the R2-NCA monomer to obtain polypeptide; primary amine or quaternary amine is modified to a polypeptide side group through click reaction of sulfydryl and double bonds, and finally the antibacterial polypeptide high polymer material is prepared. The antibacterial polypeptide high polymer material with a specific structure prepared by the method can effectively penetrate through and resist a biological membrane matrix barrier and specifically target bacterial membrane phospholipids; the compound shows excellent in-vivo antibacterial activity, can effectively inhibit biofilm-related infection and promote repair of infected parts, and has good treatment effect and application prospect.

The invention discloses a nano prodrug with broad-spectrum antibacterial activity as well as a preparation method and application of the nano prodrug, and relates to the technical field of nano medicines. A compound phenylboronic acid modified cibifloxacin is prepared by sequentially carrying out reactions with di-tert-butyl dicarbonate, 4-bromomethyl phenylboronic acid and hydrochloric acid, and the compound is subjected to a dialysis method to prepare the nano prodrug phenylboronic acid cibifloxacin (CPBP). In an infected microenvironment, CPBP can be activated by excessive hydrogen peroxide (H2O2) in the microenvironment to release an original drug cibifloxacin, broad-spectrum antibacterial activity is generated, and a specific bactericidal effect is achieved at an infected part.

The invention discloses a pH response type pocket molecule functionalized antibacterial implant as well as a preparation method and application thereof. The preparation method comprises the steps of alkali heat treatment of the titanium-based implant, silane coupling agent surface modification, pocket molecule grafting and antibacterial agent loading. The pocket molecules are modified on the surface of the implant through a'thiol-ene 'click reaction, so that the implant is endowed with dual functions of pH-responsive antibiosis and tissue repair promotion. On one hand, the pocket molecules serve as an antibacterial agent carrier and can release an antibacterial agent in an acidic environment at an infected part, so that bacteria are effectively killed and biofilm formation is inhibited; and on the other hand, the cholic acid compounds in the pocket molecules endow the implant with excellent biological activity, so that the prepared implant has excellent stability, pH responsiveness, antibacterial property and biocompatibility, can avoid high cytotoxicity caused by burst release of an antibacterial agent, is suitable for orthopedic implant infection prevention and treatment, and has wide application prospects. The important clinical application value is realized.

The invention belongs to the technical field of radiopharmaceutical chemistry and clinical nuclear medicine, and particularly relates to a D-amino acid derivative as well as a preparation method and application thereof. A radioactive preparation obtained by labeling the D-amino acid derivative with radionuclide has high uptake in a bacterial infection part, is good in infection / non-target ratio, can be specifically combined with bacteria, and can distinguish aseptic inflammation. The radioactive preparation provided by the invention can be used as a novel radioactive drug for diagnosis of bacterial infection, and has good clinical popularization and application prospects and value.

The application provides an N-terminal fatty acid modified antibacterial peptide analogue and application thereof. Specifically, the antibacterial peptide analogue of the application has a fatty acid (dodecanoic acid, tetradecanoic acid or octadecanoic acid) modification at the N terminus of a super short peptide Lys-Gly-Lys to form a lipopeptide substance. The N-terminal fatty acid modified antibacterial peptide analogue described in the application can inhibit a plurality of pathogenic bacteria, in particular a plurality of mastitis pathogenic bacteria, has high inhibitory activity, and has a unique antibacterial mechanism, is not easy to induce drug resistance of pathogenic bacteria, can more safely inhibit bacteria, and can also solve the problem of antibiotic resistance easily produced in the treatment process of mastitis; further, the antibacterial peptide analogue of the application can also down-regulate the expression of body pro-inflammatory factors (for example, TNF-alpha, IL-6), and accelerate the repair of infected site tissues. The antibacterial peptide analogue of the application can effectively improve the cure rate of dairy cows and reduce economic losses.

The application belongs to the technical field of medical apparatus and discloses an ultrasonic antibacterial device for treating bone infection, which comprises a main control shell and a minimally invasive catheter. The main control shell is internally provided with a guide wire control module, an ultrasonic control module and a medium storage cavity. The minimally invasive catheter is of a double-layer structure and is internally provided with a guide wire. The front end of the minimally invasive catheter is provided with an annular ultrasonic medium cavity, which is used for filling ultrasonic medium solution. The ultrasonic medium cavity is internally provided with an ultrasonic generating assembly arranged in an annular array. The ultrasonic generating assembly is internally provided with a piezoelectric wafer, which is used for realizing electric-acoustic conversion to realize emission and acceptance of ultrasonic waves. When the device is used, the infection site is accurately positioned through the guide wire, ultrasonic waves are emitted through the ultrasonic generating assembly, and the structure of bacterial biofilm is destroyed through ultrasonic cavitation effect, so that the bacterial biofilm is removed and the antibacterial treatment effect is achieved. The device is suitable for bone infection treatment.

When the probiotic vesicle gel carrying the gene editing tool is applied to body surface infection parts of pathogenic bacteria of the same species as probiotics, DNA double-strand breakage of a pathogenic bacteria genome is caused, the probiotic vesicle gel has a bactericidal effect on wild pathogenic bacteria and drug-resistant pathogenic bacteria, and drug resistance of the pathogenic bacteria is avoided. The probiotic vesicle gel carrying the gene editing tool contains the gene editing tool, probiotic vesicles and gel. The probiotic vesicle gel carrying the gene editing tool is used for body surface or mucous membrane surface antibacterial treatment, has the advantages of being high in safety, capable of resisting drug-resistant bacteria, high in antibacterial effect, easy to remove and the like, and has a good treatment effect on secondary bacterial infection after ionizing radiation injury.