Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for Determination of Phenolic Antioxidants in Edible Oils by Dispersion Liquid-Liquid Microextraction-High Performance Liquid Chromatography

A high-performance liquid chromatography and phenolic antioxidant technology, applied in the field of analytical chemistry, can solve problems such as time-consuming and large number of samples, and achieve the effect of reducing complexity

Inactive Publication Date: 2018-08-31
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are often time consuming, use large amounts of organic solvents during the extraction process and require large amounts of sample

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for Determination of Phenolic Antioxidants in Edible Oils by Dispersion Liquid-Liquid Microextraction-High Performance Liquid Chromatography
  • Method for Determination of Phenolic Antioxidants in Edible Oils by Dispersion Liquid-Liquid Microextraction-High Performance Liquid Chromatography
  • Method for Determination of Phenolic Antioxidants in Edible Oils by Dispersion Liquid-Liquid Microextraction-High Performance Liquid Chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The method for determining the type and content of phenolic antioxidants in edible oil by dispersion liquid-liquid microextraction-high performance liquid chromatography, the specific steps are as follows:

[0031] 1. Sample pretreatment process: dispersion liquid-liquid microextraction

[0032] Weigh 25 mg of the edible oil sample into a 2 mL centrifuge tube equipped with a screw cap. Then quickly add 0.5mL n-hexane dispersant to increase the fluidity of edible oil. After thorough mixing, quickly inject 0.5mL of acetonitrile extractant into the sample, then sonicate for 2min, and then centrifuge at 3000r / min for 2min. After centrifugation, transfer the extract phase into a sample vial.

[0033] 2. Analysis and determination of the retention time and content of each component in the extraction phase by high performance liquid chromatography

[0034] High performance liquid chromatography analysis conditions: the mobile phase is acetonitrile (A) and water (B) containi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method of measuring varieties and contents of phenol antioxidants in edible oil by utilizing dispersive liquid liquid microextraction-high performance liquid chromatography is disclosed. The method includes 1) mixing the edible oil to be measured with a dispersing agent, adding an extractant, mixing and collecting an extract phase, and 2) measuring retention time and the content of each component in the extract phase by utilizing high performance liquid chromatography, comparing with retention time of phenol antioxidant standard substances and calculating the contents, thus obtaining the varieties and the contents of the phenol antioxidants in the edible oil. The method is characterized by being rapid, sensitive and low in organic solvent using, can measure the varieties and the contents of the phenol antioxidants in the edible oil by only detecting a small amount of an edible oil sample to be measured, and can be used for phenol antioxidant detection in a plurality of types of edible oil.

Description

technical field [0001] The invention relates to a method for determining the type and content of phenolic antioxidants in edible oil by dispersion liquid-liquid microextraction-high performance liquid chromatography, and belongs to the field of analytical chemistry. Background technique [0002] As a food additive, phenolic synthetic antioxidants have been widely used to prevent or delay the oxidative deterioration of oil during transportation and storage. Commonly used phenolic synthetic antioxidants in the world include propyl gallate (PG), tert-butyl hydroquinone (TBHQ), butyl hydroxyanisole (BHA), dodecyl gallate (DG), octyl gallate (OG) and 2,6-di-tert-butyl-4-methylphenol (BHT), which can be used alone or in combination. However, many studies have shown that these substances have certain toxicity and carcinogenic effects, and excessive intake is harmful to people's health. Therefore, the amount of synthetic antioxidants used in food is strictly controlled, and the li...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 徐双娇马磊魏守军王延琴周大云匡猛方丹杨伟华
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI