Seawater bacteria detection system based on multi-wavelength laser-induced bacterial intrinsic fluorescence
A seawater bacteria and detection system technology, applied in the direction of fluorescence/phosphorescence, measuring devices, material excitation analysis, etc., can solve the problems of strict sampling requirements and difficult bacteria detection, so as to prevent corrosion, improve precision and improve detection efficiency Effect
Active Publication Date: 2020-06-23
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology
Due to the need to collect and prepare samples for these technologies, strict requirements for sample injection, and the need to make fluorescent markers, it is difficult to apply to the detection of bacteria in the marine field
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[0016] A schematic diagram of a seawater bacteria detection system based on multi-wavelength laser-induced intrinsic fluorescence of bacteria in this embodiment is as follows figure 1 As shown, it includes an outer casing 1, a first convex lens 2, a multi-wavelength laser excitation device 3, an optical fiber body 4, a fluorescence spectrometer 5 and a data processing and analysis system; the first convex lens 2 is placed in the outer casing 1 and the outer casing 1 is divided into a seawater chamber 11 and a light collection chamber 12, the inner wall of the seawater chamber 11 is provided with a window glass 13 except where the first convex lens 2 is located, and the multi-wavelength laser excitation device 3 is provided between the window glass 13 and the outer casing 1 Between, the outer casing 1 is provided with a water inlet 14 and a water outlet 15 communicating with the seawater chamber 11; the light collection chamber 12 is provided with a light collection port 16, and...
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The invention relates to a seawater bacteria detection system based on multi-wavelength laser inducing bacteria inherent fluorescence. The system includes an outer shell, a first convex lens, a multi-wavelength laser excitation device, an optical fiber body, a fluorescent spectrum instrument, and a data process and analysis system. The first convex lens is arranged in the outer shell and divides the outer shell into a seawater chamber and a light collection chamber. A window glass is arranged on an inner wall, except the position that the first convex lens is arranged, of the seawater chamber. The multi-wavelength laser excitation device is disposed between the window glass and the outer shell. A water inlet and a water outlet are arranged on the outer shell and are communicated with the seawater chamber. The light collection chamber is provided with a light collection port. The centers of the first convex lens, the light collection port and the optical fiber body are located in the same straight line. The optical fiber body receives a fluorescent signal from the light collection port. The data process and analysis system is used for processing and analyzing the fluorescent signal that is sent from the optical fiber body and is collected by the fluorescent spectrum instrument. The system can be used for on-situ detection of abundance and diversity of bacteria in seawater in ocean, is effectively improved in detection efficiency and is convenient and quick to use.
Description
technical field [0001] The present invention relates to the technical field of marine bacteria detection system, more specifically, to a seawater bacteria detection system based on multi-wavelength laser-induced intrinsic fluorescence of bacteria. Background technique [0002] Due to the unculturability of most marine bacteria and their inadaptability to high temperature and low pressure in the laboratory, in situ measurement is of great significance for a more comprehensive understanding of the abundance and diversity of marine bacteria. Due to its high sensitivity, fluorescence detection technology has always been in a very important position in bacterial research. At present, the commonly used fluorescence detection technology in the application of bacteria mainly includes fluorescence microscopy, flow cytometry, rapid detection of ATP fluorescence, fluorescent protein labeling and so on. Because these techniques have defects such as the need to collect and prepare sampl...
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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 杨顶田张敬祥
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI