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Propolis extract and extraction process thereof

A propolis extract and an extraction process technology are applied in directions such as medical preparations containing active ingredients, unknown raw materials, pharmaceutical formulations, etc., and can solve problems such as low solubility, low volatile oil content, and inability to meet the needs of actual production, and reduce Energy consumption and the effect of improving extraction efficiency

Active Publication Date: 2016-11-09
SHENZHEN VIGOR HEALTH CARE PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] It can be seen from the above published patents that the current extraction of propolis is mainly based on ethanol as a solvent, soaking at room temperature or reflux extraction, but according to the principle of similar miscibility, the weakly polar components in the volatile oil, such as terpenoids and alkenes, etc. Hydrocarbons are less soluble in highly polar ethanol (or ethanol aqueous solution), so the content of volatile oil in the propolis extracted from the propolis disclosed in the above-mentioned patent is extremely low, which cannot meet the requirements of actual production. need

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of propolis extraction process, it comprises the steps:

[0033] 1) Take propolis raw material, remove impurities, freeze at -20°C for 10 hours; then pulverize, pass through a 100-mesh sieve to obtain fine powder, and set aside;

[0034] 2) Add the mixed solvent to the fine powder according to the ratio of fine powder: mixed solvent = 1:22W / V, ultrasonically treat for 60 minutes at 30°C, then filter to obtain the filter residue and filtrate, and concentrate the filtrate under reduced pressure to obtain solid 1;

[0035] Wherein, the mixed solvent is n-hexane: ethyl acetate: 95% ethanol aqueous solution=1:1:0.1V / V / V;

[0036] 3) Add ethanol aqueous solution to the filter residue obtained in step 2) according to the ratio of filter residue:75% ethanol aqueous solution=1:5W / V and extract at 65° C. for 2 times, each time for 2 hours, and filter the extract to obtain the filtrate;

[0037] 4) Concentrate the filtrate under reduced pressure to obtain solid 2 and solid...

Embodiment 2

[0039] A kind of propolis extraction process, it comprises the steps:

[0040] 1) Take 1 kg of propolis raw material, remove impurities, freeze at -20°C for 6 hours, crush, continue to freeze at -20°C for 6 hours, crush, pass through a 100-mesh sieve to obtain a fine powder, and set aside;

[0041]2) Add the mixed solvent to the fine powder according to the ratio of fine powder: mixed solvent = 1:22W / V, ultrasonically treat for 60 minutes at 30°C, then filter to obtain the filter residue and filtrate, and concentrate the filtrate under reduced pressure to obtain solid 1;

[0042] Wherein, the mixed solvent is n-hexane: ethyl acetate: 95% ethanol aqueous solution=1:1:0.1V / V / V;

[0043] 3) Add ethanol aqueous solution to the filter residue obtained in step 2) according to the ratio of filter residue:75% ethanol aqueous solution=1:5W / V and extract at 65° C. for 2 times, each time for 2 hours, and filter the extract to obtain the filtrate;

[0044] 4) Concentrate the filtrate und...

Embodiment 3

[0047] A kind of propolis extraction process, it comprises the steps:

[0048] 1) Take 1 kg of propolis raw material, remove impurities, freeze at -20°C for 4 hours, and crush it into 25 cm 3 Blocks of large or small sizes; continue to freeze at -20°C for 2 hours, then pulverize, and pass through a 20-mesh sieve to obtain a coarse powder; finally freeze at -20°C for 4 hours, crush and pass through an 80-mesh sieve to obtain a fine powder, and set aside.

[0049] 2) Add the mixed solvent to the fine powder according to the ratio of fine powder: mixed solvent = 1:22W / V, ultrasonically treat for 60 minutes at 30°C, then filter to obtain the filter residue and filtrate, and concentrate the filtrate under reduced pressure to obtain solid 1;

[0050] Wherein, the mixed solvent is n-hexane: ethyl acetate: 95% ethanol aqueous solution=1:1:0.1V / V / V;

[0051] 3) Add 3 grams of soybean lecithin to the filter residue obtained in step 2), then add ethanol aqueous solution according to the...

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Abstract

The invention belongs to the technical field of propolis extraction, and in particular relates to a propolis extract and an extraction process thereof. The extraction process comprises the following steps: 1) removing impurities from a propolis raw material, freezing at 10 DEG C to minus 20 DEG C, crushing, and screening so as to obtain fine powder for later use; 2) adding a mixed solvent into the fine powder, performing ultrasonic treatment for 30-60 minutes at 15-35 DEG C, filtering so as to obtain filtrate residues and filtrate, and performing depressurization concentration on the filtrate so as to obtain a solid 1; 3) adding an ethanol solution into the filtrate residues obtained in the step 2), extracting for 1-3 times at 65-75 DEG C, 1-3 hours each time, and filtering the extract so as to obtain a filtrate; 4) performing depressurization concentration on the filtrate, combining the obtained solid 2 with the solid 1, and drying, thereby obtaining the propolis extract. The propolis obtained by using the extraction process provided by the invention is high in content of ketone and volatile oil, and low in content of impurities (such as lead).

Description

technical field [0001] The invention belongs to the technical field of propolis extraction and separation, and in particular relates to a propolis extract and an extraction process thereof. Background technique [0002] Propolis is a kind of viscous substance which is collected by western bees from plant resin, mixed with beeswax, pollen and bee gland secretions, and chewed by bees. It has a pleasant aromatic smell. Bees use propolis to fill the gaps in the hive, smooth the inner wall of the hive, and use propolis to prevent the decay of foreign objects in the hive and the infection of germs. [0003] The composition of propolis is complex, consisting of about 55% resin and tree fragrance, about 30% beeswax, 10% aromatic volatile oil and 5% pollen and sundries. Due to different collagen plants in different regions, the chemical composition of propolis produced varies greatly. Propolis has the texture specificity of being hard and brittle at low temperature and viscous at h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/644
CPCA61K35/644
Inventor 刘兵于荣贤余玉芳张勇王祖忠孙小玲
Owner SHENZHEN VIGOR HEALTH CARE PROD CO LTD
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