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Technique for extracting fructus trichosanthis seed crude protein

An extraction process, the technology of Trichosanthes seeds, which is applied in the field of crude protein extraction of Trichosanthes seeds, can solve the problems in the initial stage, and achieve the effects of simple operation, convenient industrialization and high yield of crude protein

Inactive Publication Date: 2008-07-09
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the protein extraction process of Trichosanthes seeds is still in its infancy. Therefore, the development of a scientific extraction process will greatly promote and promote the research and application of Trichosanthes seeds protein in food and medical treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Raw materials, reagents and equipment

[0020] 1) The raw material of Trichosanthes seeds for testing in the present invention is provided by Hunan Weilong Trichosanthes Company.

[0021] 2) The reagents and equipment are as follows:

[0022] Reagents: liquid carbon dioxide, sodium hydroxide (analytical grade), sodium chloride (analytical grade), dilute hydrochloric acid, distilled water.

[0023] Equipment: HA121-50-01C type supercritical extraction device system, Yuyao Dongfang Electrician Instrument Factory HH.W21.420 electric heating constant temperature water tank, Shanghai-Heng Scientific Instrument Co., Ltd. DHG-9620A electric heating constant temperature blast drying oven, SevenEasy Type precision acidity meter, LD5-10 desktop centrifuge, TDZ4B low-speed centrifuge, AUY120 electronic analytical balance.

[0024] 2. Extraction process of Trichosanthes seed crude protein

[0025] 1) Using supercritical CO 2 oil extraction

[0026] Take 100 grams of dried T...

Embodiment 2

[0031] 1. Using supercritical CO 2 oil extraction

[0032] Get 100 grams of dried Trichosanthes seeds, pass through a 40-mesh sieve after crushing, place in a supercritical extraction kettle to extract oil, the supercritical extraction conditions are: extraction pressure is 35MPa, separation pressure is 9MPa, extraction temperature is 55°C, separation temperature 45°C, CO 2 The flow rate is 60L / h, the extraction time is 3h, and the oil is recovered.

[0033] 2. Extract protein from the remaining dross by alkali-soluble acid precipitation

[0034] Add 2% sodium hydroxide solution to the feed liquid residue after oil extraction, wherein the mass volume ratio (w / v) of the feed liquid residue to the dilute sodium hydroxide solution is 1:10, and extract at 60° C. for 75 min.

[0035] The pH value of the above extract was adjusted to 3.8 with 1.5 mol / L hydrochloric acid, and settled at 4°C for 14 hours. Centrifuge at 1600r / min for 15min at 3°C, the crude protein extract weighs 8...

Embodiment 3

[0037] 1. Using supercritical CO 2 oil extraction

[0038] Get 100 grams of dried Trichosanthes seeds, pass through a 20-mesh sieve after crushing, place in a supercritical extraction kettle to extract oil, the supercritical extraction conditions are: extraction pressure is 15MPa, separation pressure is 9MPa, extraction temperature is 45°C, separation temperature 55°C, CO 2 The flow rate is 70L / h, the extraction time is 4h, and the oil is recovered.

[0039] 2. Extract protein from the remaining dross by alkali-soluble acid precipitation

[0040] Add 3% sodium hydroxide solution to the feed liquid residue after oil extraction, wherein the mass volume ratio (w / v) of the feed liquid residue to the dilute sodium hydroxide solution is 1:10, and extract at 70° C. for 90 min.

[0041] The pH value of the above extract was adjusted to 4.0 with 1.2 mol / L hydrochloric acid, and settled at 4°C for 14 hours. Centrifuged at 1400r / min for 25min at 3°C, the crude protein extract weighed...

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Abstract

The invention discloses an extraction technology of melon seeds coarse protein, which comprises firstly screening melon seeds after dried and disintegrated, and extracting grease through supercritical CO2 extracting method, secondly extracting protein from residue through the method of alkali dissolving and acid deposition, and then obtaining coarse protein extract through cryopreservation and centrifugation after acid deposition and then drying coarse protein, wherein technology parameters are that the weight volume ratio (w / v) of residue in feed liquid and dilute lye is 1:5-1:40, the mass concentration of dilute lye is 0.5-5%, the extraction time is 30-120min, and the extraction temperature is 40-80 DEG C. The invention has practicable and simple technology and high coarse protein yield, which is convenient for industrialized carrying out.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, and in particular relates to a process for extracting crude protein from Trichosanthes quince seeds. Background technique [0002] Gualou is also known as Tiangua, Yaogua, wild bitter gourd, Trichosanthes etc. It is a perennial herbaceous vine of the genus Cucurbitaceae. The whole body of Gualou is full of treasures: the fruit, stems and leaves, and roots can be used as medicine. The whole dried fruit is named Quangualou in traditional Chinese medicine, the shell is called Trichosanthes skin, and the seeds are called Trichosanthes seeds. Intestinal smoothing effect, mainly treats symptoms such as phlegm-heat cough, lung phlegm hemoptysis, chest tightness, diarrhea, and constipation. [0003] Modern medical research has proved that: Trichosanthes seeds can expand the coronary arteries of the heart, increase coronary flow, have obvious protective effects on acute myocardial ischemia, a...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14
CPCY02P20/54
Inventor 李赤翎
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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