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Preparing method of group A and group C meningococcus and haemophilus influenzae type b combined vaccine

A technology for Haemophilus influenzae and meningococcus, which is applied in the biological field to achieve the effects of convenient preparation, reduction of inoculation times, and reduction of inoculation cost

Inactive Publication Date: 2016-11-09
CHENGDU OLYMVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, pharmacological preventive measures alone are not sufficient to control the disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: A preparation method of a group A group C meningococcal group b Haemophilus influenzae type b combined vaccine, which comprises the following steps:

[0020] S1. Preparation of monovalent vaccine stock solution: including preparation of group A meningococcal conjugate vaccine stock solution, preparation of group C meningococcal conjugate vaccine stock solution, preparation of Haemophilus influenzae type b conjugate vaccine stock solution;

[0021] S11. Preparation of stock solution of group A meningococcal conjugate vaccine

[0022] Open the working seed batch of meningococcus group A, inoculate it to the meningococcal semi-comprehensive medium at a temperature of 35~37℃, cultivate for 16~24 hours, then after three generations of expansion and culture, prepare a suitable quantity of production seeds and inoculate to Cultivate in 500L fermenter, temperature 35~37℃, add formaldehyde after 6~12 hours of culture time for sterilization;

[0023] Centrifuge the sterilized ...

Embodiment 2

[0041] Example 2: A preparation method of a group A group C meningococcal group b Haemophilus influenzae type b combined vaccine, which comprises the following steps:

[0042] S1. Preparation of monovalent vaccine stock solution: including preparation of group A meningococcal conjugate vaccine stock solution, preparation of group C meningococcal conjugate vaccine stock solution, preparation of Haemophilus influenzae type b conjugate vaccine stock solution;

[0043] S11. Preparation of stock solution of group A meningococcal conjugate vaccine

[0044] Open the working seed batch of meningococcus group A, inoculate it to the meningococcal semi-comprehensive medium at a temperature of 35~37℃, cultivate for 16~24 hours, then after three generations of expansion and culture, prepare a suitable quantity of production seeds and inoculate to Cultivate in 500L fermenter, temperature 35~37℃, add formaldehyde after 6~12 hours of culture time for sterilization;

[0045] Centrifuge the sterilized ...

Embodiment 3

[0063] Example 3: A preparation method of a group A group C meningococcal group b Haemophilus influenzae type b combined vaccine, which comprises the following steps:

[0064] S1. Preparation of monovalent vaccine stock solution: including preparation of group A meningococcal conjugate vaccine stock solution, preparation of group C meningococcal conjugate vaccine stock solution, preparation of Haemophilus influenzae type b conjugate vaccine stock solution;

[0065] S11. Preparation of stock solution of group A meningococcal conjugate vaccine

[0066] Open the working seed batch of meningococcus group A, inoculate it to the meningococcal semi-comprehensive medium at a temperature of 35~37℃, cultivate for 16~24 hours, then after three generations of expansion and culture, prepare a suitable quantity of production seeds and inoculate to Cultivate in 500L fermenter, temperature 35~37℃, add formaldehyde after 6~12 hours of culture time for sterilization;

[0067] Centrifuge the sterilized ...

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Abstract

The invention discloses a preparing method of group A and group C meningococcus and haemophilus influenzae type b combined vaccine. The method includes the steps of preparing a monovalent vaccine stock solution, preparing the combined vaccine and freeze-drying the vaccine. The invention provides the preparing method of the group A and group C meningococcus and haemophilus influenzae type b combined vaccine, group A and group C meningococcus and haemophilus influenzae type b are combined together, and the prepared combined vaccine is remarkable in effect; as the vaccine is of combined type, the number of times of inoculation is reduced, infant trauma is reduced, the inoculation cost is reduced, and the combined vaccine has good social and economic benefits. The method is easy to operate and suitable for large-scale industrial production, and the combined vaccine is easy to prepare and low in cost.

Description

Technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a preparation method of a group A group C meningococcus type b Haemophilus influenzae combined vaccine. Background technique [0002] Meningococcal meningitis is an acute respiratory infection caused by Neisseria meningitidis. For more than one hundred years, it has been circulating or sporadic all over the world, and infection with pathogenic bacteria can cause sepsis and meningitis. The susceptible population is mainly children, with the highest fatality rate of fulminant type, which can reach 40% to 60%. Today, the incidence rate in all continents of the world is 1 / 100,000 to 10 / 100,000, and the total case fatality rate is 5% to 15%. Up to 20% of meningitis patients will have neurological sequelae, including mental impairment and deafness. [0003] Meningococcus can be classified into 13 serotypes according to the type of capsular polysaccharide. Among them, group A,...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/102A61K39/095A61K9/19A61P31/04
CPCA61K39/102A61K9/19A61K39/095A61K47/26A61K2039/55A61K2039/70
Inventor 吴强陈道远李晟陈元芬
Owner CHENGDU OLYMVAX BIOPHARM
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