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Method for extracting kasugamycin hydrochloride by kasugamycin fermentation broth

A technology of kasugamycin hydrochloride and kasugamycin, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of low effective content, unfavorable product export, difficult long-term storage, transportation and use, and achieve Improve yield, facilitate storage, and facilitate long-distance transportation

Active Publication Date: 2016-11-09
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 2 Relevant literature reports the method for preparing kasugamycin, but the effective content of the product is low, which is not conducive to the export of the product
[0005] 3 Relevant literatures reported that Kasugamycin was prepared by spray-drying method, and the product was not easy to be stored, transported and used for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Kasugamycin fermentation broth 100m 3 , The chemical potency is 15201u / ml.

[0046] 1) Fermentation broth pretreatment

[0047] Use 20% hydrochloric acid solution to adjust the pH of the kasugamycin fermentation broth to 5, stir for 20 minutes, and let stand for 40 minutes; continue to adjust the pH of the kasugamycin fermentation broth to 3, stir for 20 minutes, and let stand for 40 minutes.

[0048] Add 100 kg of polysilicon aluminum calcium chloride to the fermentation broth, continue to stir for 20 minutes, and let stand for 60 minutes.

[0049] 2) Ceramic membrane filtration

[0050] Drinking water is added to the pretreated fermentation broth, the viscosity drops to 2943Pa·s, and the temperature of the fermentation broth is raised to 50°C.

[0051] In the process of membrane filtration, high-speed cross-flow method is used for membrane filtration. During the filtration process, the pressure is controlled at 0.2-0.3MPa, and the flow rate is controlled at 0.5m 3...

Embodiment 2

[0064] Kasugamycin fermentation broth 100m 3 , The chemical potency is 14936u / ml.

[0065] 1) Fermentation broth pretreatment

[0066]Use 22% hydrochloric acid solution to adjust the pH of the kasugamycin fermentation broth to 4.8, stir for 22 minutes, and let stand for 45 minutes; continue to adjust the pH of the kasugamycin fermentation broth to 2.8, stir for 22 minutes, and let stand for 45 minutes.

[0067] Add 150 kg of polysilicon aluminum calcium chloride to the fermentation broth, continue stirring for 22 minutes, and let stand for 65 minutes.

[0068] 2) Ceramic membrane filtration

[0069] Drinking water is added to the pretreated fermented liquid, the viscosity drops to 2893 Pa·s, and the temperature of the fermented liquid is raised to 52°C.

[0070] In the process of membrane filtration, high-speed cross-flow method is used for membrane filtration. During the filtration process, the pressure is controlled at 0.2-0.3MPa, and the flow rate is controlled at 0.6m ...

Embodiment 3

[0083] Kasugamycin fermentation broth 100m 3 , The chemical potency is 15106u / ml.

[0084] 1) Fermentation broth pretreatment

[0085] Use 25% hydrochloric acid solution to adjust the pH of the kasugamycin fermentation broth to 4.7, stir for 25 minutes, and let stand for 50 minutes; continue to adjust the pH of the kasugamycin fermentation broth to 2.7, stir for 25 minutes, and let stand for 50 minutes.

[0086] Add 200 kg of polysilicon aluminum calcium chloride to the fermentation broth, continue to stir for 25 minutes, and let stand for 70 minutes.

[0087] 2) Ceramic membrane filtration

[0088] Drinking water is added to the pretreated fermented liquid, the viscosity drops to 2851Pa·s, and the temperature of the fermented liquid is raised to 53°C.

[0089] During the membrane filtration process, high-speed cross-flow method is used for membrane filtration. During the filtration process, the pressure is controlled at 0.2-0.3MPa, and the flow rate is controlled at 0.7m ...

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PUM

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Abstract

The invention relates to a method for extracting kasugamycin hydrochloride by kasugamycin fermentation broth. The method comprises the following technical steps: carrying out preprocessing, ceramic membrane filtering, macroporous resin decoloration, concentration by nanofiltration, crystallization and drying on the kasugamycin fermentation broth. Compared with the domestic conventional production technology, the method has the advantages that production cost is lowered, the quality of a finished product is improved, and the method is favorable for enhancing the domestic and foreign market competitiveness of a product.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, in particular to a method for extracting kasugamycin hydrochloride by using kasugamycin fermentation liquid. Background technique [0002] Kasugamycin is a weakly basic antibiotic produced by aureus streptomycin, which is a dual-use antibiotic for agriculture and medicine. At present, relevant domestic literatures report the method of adding oxalic acid to the kasugamycin fermentation broth for pretreatment and extracting kasugamycin by macroporous resin technology. The main problems that exist are: [0003] 1 The addition of oxalic acid in the pretreatment process of kasugamycin fermentation broth increases the production cost. [0004] 2 Related literatures reported the method of preparing kasugamycin, but the effective content of the product was low, which was not conducive to the export of the product. [0005] 3 Relevant literatures reported that kasugamycin was prepared by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/207C07H1/06
CPCC07H1/06C07H15/207
Inventor 任勇王隽梅
Owner 宁夏泰瑞制药股份有限公司