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On-chip cell detecting system and method based on PDMS chip

A cell and chip technology, applied in the field of biomedicine, can solve the problems of expensive instruments and complex systems, and achieve the effects of convenient tuning and simple preparation process.

Active Publication Date: 2016-11-09
SHANGHAI CRIMINAL SCI TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its detection parameters can be light scattering or fluorescence indicators; but before that, it is necessary to prepare fluorescein-labeled antibodies and stain the cells, the whole system is complicated and the instrument is expensive

Method used

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  • On-chip cell detecting system and method based on PDMS chip
  • On-chip cell detecting system and method based on PDMS chip
  • On-chip cell detecting system and method based on PDMS chip

Examples

Experimental program
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Embodiment 1

[0029] Such as figure 1 and figure 2 As shown, in the on-chip cell discrimination and sorting device based on the PDMS chip involved in this embodiment, the first inlet 1-1 is the cell liquid to be sorted, and the second and third inlets 1-2 and 1 ‐3 is the sheath fluid channel. The first injection port 1-1 with a rectangular cross-section is located between the second injection port 1-2 and the third injection port 1-3, and the side length of the rectangular structure is from the second injection port to the third injection port. From the merged end of the sample port to the merged end of the first sample outlet 1-9 and the first sample outlet 1-10, it gradually decreases.

[0030] The sample is loaded in a syringe and injected into the PDMS chip by a registered pump, and the injection speed is controlled. The sheath fluid forms a wrapping and squeezing effect on the sample flow, so that the cell fluid to be sorted is focused and passed through the detection area, and at ...

Embodiment 2

[0035] Such as image 3As shown, in the detection system including the on-chip cell discrimination and sorting device based on the PDMS chip involved in this embodiment, the wavelength of the tunable laser 3-5 is 1550 nm, which is consistent with the transmission characteristic wavelength of the FP cavity. The Bragg gratings at one end of fibers 3-6 and 3-7 face each other to form an FP cavity, and characteristic transmission occurs within the Bragg reflection wavelength bandwidth. The power detector 3-8 detects the change of the transmitted light power. The microchannel in the chip is in the shape of fire, and the FP cavity is in the straight-through to the middle section. One is to make the cell fluid and sheath fluid at the front end form a stable laminar flow, and the middle layer of the cell fluid is gradually narrowed; the other is that the opening and closing of the micro-air valve at the end requires a response time. The cell fluid and sheath fluid used in the experi...

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Abstract

The invention provides an on-chip cell detecting system and method based on a PDMS chip. The system comprises an on-chip cell distinguishing and sorting device, and an injection pump, a gas pump, a tunable laser and a power detector which are connected with the on-chip cell distinguishing and sorting device. A sample outlet and a sample inlet of the on-chip cell distinguishing and sorting device are connected with the injection pump. A port of a micro-channel gas valve is connected with the gas pump. The input end and the output end of an optical fiber Bragg grating FP resonant cavity of the on-chip cell distinguishing and sorting device are connected with the tunable laser and the power detector respectively. The deformation of a PDMS thin film interlayer of the on-chip cell distinguishing and sorting device is controlled through the gas pump, and closing / opening is achieved. By means of the light detecting method, cell samples are not damaged, and the pretreatment such as dyeing or fluorescent marking of cell samples can be avoided.

Description

technical field [0001] The invention relates to a detection technology in the field of biomedicine, in particular to an on-chip cell detection system and method based on a PDMS chip. Background technique [0002] Flow cytometry (Flow Cytometry) is a detection method for rapid determination, sorting, collection and quantitative analysis of single cells or other biological particles. Flow cytometer, as the detection technology platform, is a modern biomedical instrument. Its detection parameters can be light scattering or fluorescent indicators; however, it is necessary to prepare fluorescein-labeled antibodies and stain the cells before, and the whole system is complicated and the equipment is expensive. [0003] Optofluidic is the crystallization of optics and microfluidic technology, so microfluidic devices are also called optical chips or lab on a chip (Lab on a chip). Its microanalysis system uses very little sample reagents, high resolution and sensitivity, low cost, a...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCB01L3/5027B01L2300/0867
Inventor 邹芸刘文斌杨飞宇江蓓梁培斯陈险峰
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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