Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sheep characteristic polypeptide and application thereof

A sheep-derived, sheepskin technology, applied in the field of detection and application, can solve the problems of non-specificity of characteristic polypeptides, incomplete enzyme digestion, and inability to find characteristic polypeptides, etc., and achieves strong characteristics, high sensitivity, and simple operation. Effect

Inactive Publication Date: 2016-11-09
SHANDONG INST FOR FOOD & DRUG CONTROL
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to incomplete digestion, the detected characteristic peptides may not be completely digested peptides; in addition, due to the limitation of mass spectrometry scanning conditions, it is impossible to scan all the digested peptides, which may cause the characteristic peptides found. Peptides are not specific or can not find the real characteristic peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sheep characteristic polypeptide and application thereof
  • Sheep characteristic polypeptide and application thereof
  • Sheep characteristic polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Screening and determination of sheep-derived characteristic peptides:

[0032]Collagen is the main protein in animal dermis, so the present invention selects suitable polypeptides from collagen as characteristic polypeptides of sheepskin collagen. Use the PeptideMass function provided by Uniport to obtain the results of trypsin enzymatic hydrolysis of donkey hide, horsehide, cowhide, sheepskin, and pigskin collagen; use the molecular biology software MEGA5. The protein sequences were compared to obtain a sheep characteristic polypeptide with species specificity relative to donkeys, horses, cattle, and pigs, and the sequence was GFPGSDGVAGPK. In order to verify the characteristic peptide GFPGSDGVAGPK of cowhide collagen, donkey hide, horse hide, mule hide, cowhide, sheep hide, and pig hide were selected. According to the sample pretreatment method and nano-LC-ESILTQ-Orbitrap MS / MS Mass spectrometry analysis method. The test results show that ion m / z552.7699 (double cha...

Embodiment 2

[0037] Multiple reaction monitoring (MRM) detection of ingredients of sheep origin in leather samples

[0038] Donkey hide, horse hide, mule hide, cowhide, sheepskin, and pigskin were selected, according to the sample pretreatment method and HPLC-TQ-S MS / MS analysis method described above. The primary ion and secondary product ion of the characteristic peptide GFPGSDGVAGPK (3-hydroxylation) are not detected in donkey skin, horse skin, mule skin, cowhide, and pig skin, but can be detected in sheep skin, indicating that the characteristic peptide can be used in sheep skin identification.

Embodiment 3

[0040] Multiple reaction monitoring (MRM) detection of sheep-derived components in pure gum

[0041] Colloidal traditional Chinese medicine is made by boiling animal skin at high temperature for a long time. In the process of boiling at high temperature, collagen inevitably participates in a series of reactions, which leads to the denaturation and hydrolysis of collagen. In order to verify the present invention To detect the feasibility of sheep-derived components in glue samples, donkey skin, horse hide, mule hide, cowhide, sheepskin, and pigskin were boiled to make glue. According to the gel sample pretreatment method and HPLC-TQ-S MS / MS analysis method described above. The primary ions and secondary product ions of the characteristic peptide GFPGSDGVAGPK (3-hydroxylation) are not detected in donkey hide glue, horse hide glue, mule hide glue, cow hide glue, and pig hide glue, but can be detected in sheep hide glue, indicating that The characteristic peptide GFPGSDGVAGPK (3-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sheep characteristic polypeptide and application of the sheep characteristic polypeptide in detection of sheep-derived components in skin and glue Chinese medicines. The amino acid sequence of the characteristic polypeptide provided by the invention is GFPGSDGVAGPK (3-position proline hydroxylation). The polypeptide is a specific component of sheep skin collagen. Besides, the invention provides an identification method of sheep skin. The identification method comprises the following steps of treating skin by adopting trypsin; detecting the characteristic peptide by adopting liquid chromatography-tandem mass spectrometry, thereby identifying whether the skin is the sheep skin or not. The invention also provides a method for detecting the sheep-derived components in glue Chinese medicines. The method comprises the following steps of performing enzyme digestion on the glue Chinese medicines by adopting the trypsin, and enabling the characteristic polypeptide to be dissociated from collagen; detecting by adopting the liquid chromatography-tandem mass spectrometry, thereby judging whether the sheep-derived components infiltrate the glue Chinese medicine or not. The method has the advantages of strong characteristic, high sensitivity, simple operation, and the like; whether the sheep-derived components exist in the glue Chinese medicines can be accurately identified.

Description

(1) Technical field [0001] The invention relates to the field of detection and application, in particular to a sheep characteristic polypeptide and its application. (2) Background technology [0002] Donkey-hide gelatin appeared in the pre-Qin period and has become a commonly used drug in the Han Dynasty. It has the functions of nourishing blood and Qi, nourishing the skin, improving the body's immunity, etc. . In recent years, due to the sharp increase in the price of donkey-hide gelatin and the shortage of donkey skin resources, some illegal enterprises use low-priced sheepskin or donkey skin mixed with sheepskin to boil donkey-hide gelatin. Although the adulteration of donkey-hide gelatin has been frequently exposed in the media, due to the lack of effective adulteration detection methods, the adulteration phenomenon has been repeatedly banned. Therefore, it is very necessary to establish a detection method with strong specificity and high sensitivity for non-donkey-der...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/88G01N2030/027G01N2030/062G01N2030/8831
Inventor 巩丽萍石峰李雪咸瑞卿杭宝建迟连利
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com