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A kind of one-component TMB chromogenic solution and preparation method thereof

A chromogenic liquid, one-component technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor uniformity, inconvenient operation, high color background, etc., to achieve improved water solubility, convenient reading, and easy operation convenient effect

Active Publication Date: 2017-08-22
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The TMB chromogen solution used in the existing commercially available enzyme-linked immunosorbent kits is divided into two types, one is two-component, divided into A solution and B solution, and it needs to be mixed before use, because it is easy to cause manipulation during use. Inconvenient and short storage time after mixing are gradually replaced by the second type; the second type is one-component TMB, which is easy to operate, but the single-component TMB currently used in the market has poor stability and short storage time. Most of the storage time is 3-6 months, and the color background is high, and the uniformity is not good

Method used

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  • A kind of one-component TMB chromogenic solution and preparation method thereof
  • A kind of one-component TMB chromogenic solution and preparation method thereof
  • A kind of one-component TMB chromogenic solution and preparation method thereof

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Embodiment 1

[0028] An embodiment of the one-component TMB chromogenic solution of the present invention, the one-component TMB chromogenic solution described in this embodiment comprises the following components: TMB, Tween 80, hydroxypropyl-β-cyclodextrin, antioxidant peptide , DMSO, disodium hydrogen phosphate, citric acid, hydrogen peroxide; wherein, the mass concentration of the TMB in the single-component TMB chromogenic solution is 0.2g / L, and the Tween 80 in the single-component The mass concentration in part TMB chromogenic solution is 1g / L, and the mass concentration of described hydroxypropyl-beta-cyclodextrin in described one-component TMB chromogenic solution is 2.5g / L, and the antioxidant peptide The molar concentration in the one-component TMB color developing solution is 1nmol / L, and the volume concentration of the DMSO in the one-component TMB color developing solution is 10ml / L, and the disodium hydrogen phosphate is in the described one-component TMB color developing solu...

Embodiment 2

[0038] An embodiment of the one-component TMB chromogenic solution of the present invention, the one-component TMB chromogenic solution described in this embodiment comprises the following components: TMB, Tween 80, hydroxypropyl-β-cyclodextrin, antioxidant peptide , DMSO, disodium hydrogen phosphate, citric acid, hydrogen peroxide; wherein, the mass concentration of the TMB in the single-component TMB chromogenic solution is 0.4g / L, and the Tween 80 in the single-component The mass concentration in part TMB chromogenic solution is 4g / L, and the mass concentration of described hydroxypropyl-beta-cyclodextrin in described one-component TMB chromogenic solution is 5g / L, and described antioxidant peptide is in The molar concentration in the one-component TMB chromogenic solution is 10nmol / L, and the volume concentration of the DMSO in the one-component TMB chromogenic solution is 20ml / L, and the disodium hydrogen phosphate is in the single-component TMB chromogenic solution. The ...

Embodiment 3

[0041] An embodiment of the one-component TMB chromogenic solution of the present invention, the one-component TMB chromogenic solution described in this embodiment comprises the following components: TMB, Tween 80, hydroxypropyl-β-cyclodextrin, antioxidant peptide , DMSO, disodium hydrogen phosphate, citric acid, hydrogen peroxide; wherein, the mass concentration of the TMB in the single-component TMB chromogenic solution is 0.1g / L, and the Tween 80 in the single-component The mass concentration in part TMB chromogenic solution is 10g / L, and the mass concentration of described hydroxypropyl-beta-cyclodextrin in described one-component TMB chromogenic solution is 4g / L, and described antioxidant peptide is in The molar concentration in the one-component TMB chromogenic solution is 20nmol / L, and the volume concentration of the DMSO in the one-component TMB chromogenic solution is 15ml / L, and the disodium hydrogen phosphate is in the single-component TMB chromogenic solution. The...

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Abstract

The invention discloses a single-component TMB coloring solution, including TMB; and the single-component TMB coloring solution also contains antioxidant peptide. The TMB coloring solution is supplemented with the antioxidant peptide as an antioxidant to prevent the oxidation of TMB, so that the TMB in the invention can be stably stored for 24 months, and can be stable for more than 3 hours after termination of the reaction. The single-component TMB coloring solution is more flexible compared with that the existing TMB needs to be read terminate within 30min after termination of the reaction. At the same time, the invention also discloses a preparation method of the single-component TMB coloring solution.

Description

technical field [0001] The invention relates to a biological in vitro diagnostic reagent and a preparation method thereof, in particular to a single-component TMB chromogenic solution and a preparation method thereof. Background technique [0002] TMB (3,3',5,5'-tetramethylbenzidine) is a new type of safe chromogen reagent, as a new substrate of peroxidase, obtained in enzyme-linked immunosorbent assay (ELISA) widely used. The substrate yields a soluble light blue end product that can be read on a spectrophotometer at 370 or 630 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution turns from colorless to yellow after adding the stop solution, and the depth of yellow reflects the amount of antigen-antibody binding in the ELISA system , so as to judge the amount of a cer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/531
CPCG01N33/531G01N33/54393
Inventor 李民友张玲李妙红蓝媛齐宗献
Owner GUANGZHOU JINDE BIOTECH
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