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A kind of ginkgo biloba extract and its preparation method and application

A technology of Ginkgo biloba and extract, which is applied in the field of medicine, can solve the problems of low utilization rate of Ginkgo biloba materials, large loss of active ingredients, cumbersome procedures of Ginkgo biloba extract, etc., and achieve reduction of sample volume, efficient adsorption and elution, and preparation The effect of simple method

Inactive Publication Date: 2017-11-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still defects and deficiencies in the above-mentioned technical scheme: (1) Ginkgo biloba extract has higher requirements on the content of lactone and flavonoids, and the content of ginkgo biloba extract lactone needs to be greater than 80%, or the content of flavonoids must be greater than 70%, and at the same time The lactone content is required to be greater than 18%; (2) the procedure for preparing the ginkgo leaf extract with high content of lactones and flavonoids is cumbersome, the loss of active ingredients is large, and the utilization rate of ginkgo leaf materials is low; (3) the pharmaceutical composition is ginkgo leaf extract and The compound prescription of β2-adrenoceptor agonist still cannot avoid adverse reactions such as skeletal muscle tremor, hypokalemia, and cardiac rhythm disorder caused by clinical use of β2-adrenoceptor agonist

Method used

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  • A kind of ginkgo biloba extract and its preparation method and application
  • A kind of ginkgo biloba extract and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of embodiment 1 Ginkgo biloba extract

[0027] (1) Ginkgo biloba leaves are dried and pulverized, and cross a 60-mesh sieve;

[0028] (2) adding 4 times of Ginkgo biloba weight, volume concentration is 65% ethanol extraction 3 times, extracting 60min each time, and merging extract;

[0029] (3) The extract is recovered under reduced pressure to obtain a concentrated solution, an equal volume of purified water is added to the concentrated solution, and precipitation is carried out for 12 hours;

[0030] (4) Take the supernatant and dry it under reduced pressure and put it on an acidic alumina column, elute with ethanol (containing 0.5% acetic acid, v / v) with a volume concentration of 90%, collect the eluent, and distill the eluent under reduced pressure to obtain The liquid extract is obtained by drying the liquid extract under reduced pressure to obtain the Ginkgo biloba extract.

Embodiment 2

[0031] The preparation method of embodiment 2 Ginkgo biloba extract

[0032] (1) Ginkgo biloba leaves are dried and pulverized, and crossed a 40-mesh sieve;

[0033] (2) adding 8 times of Ginkgo biloba weight, volume concentration is 60% ethanol extraction 2 times, extracting 60min each time, and merging extract;

[0034] (3) The extract is recovered under reduced pressure to obtain a concentrated solution, an equal volume of purified water is added to the concentrated solution, and precipitation is carried out for 15 hours;

[0035] (4) Take the supernatant and dry it under reduced pressure and put it on an acidic alumina column, elute with 80% ethanol (containing 0.2% acetic acid, v / v) with a volume concentration, collect the eluate, reclaim ethanol under reduced pressure, extract Dry under reduced pressure to obtain Ginkgo biloba extract.

Embodiment 3

[0036] The preparation method of embodiment 3 Ginkgo biloba extract

[0037] (1) Ginkgo biloba leaves are dried and pulverized, and cross a 50-mesh sieve;

[0038] (2) adding 6 times of Ginkgo biloba weight, volume concentration is 80% ethanol extraction 2 times, extracting 70min each time, and merging extract;

[0039] (3) The extract is recovered under reduced pressure to obtain a concentrated solution, an equal volume of purified water is added to the concentrated solution, and precipitation is carried out for 12 hours;

[0040] (4) Take the supernatant and dry it under reduced pressure and put it on an acidic alumina column, elute with 85% ethanol (containing 0.1% acetic acid, v / v) with a volume concentration, collect the eluate, reclaim ethanol under reduced pressure, extract Dry under reduced pressure to obtain Ginkgo biloba extract.

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Abstract

The invention relates to a ginkgo leaf extract as well as a preparation method and application thereof. The prepared ginkgo leaf extract contains 20-25wt% of gingko flavones, 2-4wt% of ginkgolides and 2-4wt% of bilobalides. The preparation method of the ginkgo leaf extract comprises the following steps: extracting ethanol; concentrating the extract, and then carrying out water precipitation; treating with an acidic alumina column; eluting with ethanol containing acetic acid; and carrying out vacuum concentration and drying on the eluent. The preparation method of the ginkgo leaf extract is simple and efficient; the prepared ginkgo leaf extract is capable of effectively relaxing the tracheal smooth muscle of a mouse so as to be used for treating asthma; and meanwhile, the ginkgo leaf extract has the advantages of low toxicity, safe medication and high safety, thereby providing a novel and safe drug for treating asthma clinically.

Description

technical field [0001] The invention relates to a ginkgo leaf extract and its preparation method and application, belonging to the technical field of medicine. Background technique [0002] Ginkgo biloba is a perennial deciduous tree of the Ginkgo family Ginkgo genus. According to Chinese Pharmacopoeia, Ginkgo biloba leaves have the functions of nourishing the lungs, relieving asthma, and relieving pain. The main active ingredients of Ginkgo biloba are flavonoids and lactones. Ginkgo biloba extract has a wide range of pharmacological effects: antagonizing platelet activating factor, scavenging free radicals, anti-inflammatory, anti-allergic, etc., and has broad application prospects. As we all know, the efficacy of Chinese medicine extracts is directly related to the composition of substances in the extracts. Different sources of medicinal materials and different extraction methods will affect the composition of substances in Chinese medicine extracts, and then affect the ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16A61P11/08A61P11/06A61K31/365
CPCA61K31/365A61K36/16A61K2236/333A61K2236/39A61K2236/55A61K2300/00
Inventor 于孟飞李帆易建明马琳
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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