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Device integrating extraction and amplification as well as detection of nucleic acid

A cavity and pipette technology, applied in the fields of nucleic acid amplification and nucleic acid detection, can solve the problems of complex device structure, fluorescent probes and expensive equipment, etc.

Active Publication Date: 2016-11-16
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these devices are complex in structure, require the use of fluorescent probes, and the equipment is expensive

Method used

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  • Device integrating extraction and amplification as well as detection of nucleic acid
  • Device integrating extraction and amplification as well as detection of nucleic acid
  • Device integrating extraction and amplification as well as detection of nucleic acid

Examples

Experimental program
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Embodiment

[0043] When in use, the user opens the sample filling hole cover 17 on the box, uses a sampler to add a certain volume of sample or pretreated sample to the extraction chamber 7 storing the sample lysate, and then covers the sample filling hole cover 17 on the sample filling chamber. On the sample hole 14, and slide the sealing plate 9 onto the sample hole. The box goes into the base unit. After the box is installed, the rotating shaft 3 and the pump push rod 1 of the box will be matched with the corresponding machinery of the machine device. There are heaters for nucleic acid amplification, constant temperature heaters for detection and movable magnets in the base unit.

[0044] Now take the extracted sample as an example to describe the specific amplification and detection steps inside the box:

[0045] 1) Move the pipette 8 directly above the extraction chamber by rotating the shaft 3 and verify this movement with the optical or magnetic sensor of the base unit.

[0046] ...

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PUM

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Abstract

The invention discloses a device integrating the extraction and the amplification as well as the detection of a nucleic acid. The device is a box, and comprises: (1) a closed cavity, (2) a pedestal, (3) at least two chambers and (4) at least one pipette, wherein the pedestal is combined with the cavity; the pedestal comprises the at least two chambers; the at least one pipette is arranged in the cavity, and can be rotated and perpendicularly moved in the cavity. The extraction, the amplification and the detection of a nucleic acid molecule can be realized in the box to which the device integrating the extraction and the amplification as well as the detection of the nucleic acid relates. Through the box, the pollution problems existing in the amplification and the detection of a pathogenic microorganism and the like can be effectively avoided.

Description

technical field [0001] The invention relates to the fields of nucleic acid amplification and nucleic acid detection, in particular to a device integrating nucleic acid extraction, amplification and detection. Background technique [0002] The nucleic acid amplification reaction realizes the amplification and detection of a small amount of nucleic acid samples, and has been widely used in biomedicine and other fields. At present, many developments have been developed, including polymerase chain reaction PCR, nucleic acid loop-mediated isothermal amplification reaction LAMP, nucleic acid sequence-dependent amplification NASBA, rolling circle amplification reaction RCA, ligation amplification reaction LCR, and helicase-dependent nucleic acid amplification. Amplification reaction HAD and other nucleic acid amplification methods. However, these target sequence-based nucleic acid amplification methods have the problem that the amplified products are prone to cross-contamination, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCB01L3/502B01L7/5255B01L2200/10B01L2300/0861C12Q1/6844
Inventor 周国华邹秉杰王建平
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A