SDS-polyacrylamide electrophoresis gel and preparation method thereof

Abstract

The invention discloses an SDS‑polyacrylamide electrophoresis gel, which is characterized in that: acrylamide monomers are mixed with a crosslinking agent methylenebisacrylamide in proportion, and triisopropanolamine, taurine, glycolic acid, A buffer system composed of glycine and tris-hydrochloric acid aqueous solution, and anionic surfactant sodium lauryl sulfate, initiator ammonium persulfate and catalyst tetramethylethylenediamine are added. The SDS-polyacrylamide electrophoresis gel prepared by the method of the present invention not only has good separation efficiency and colloidal performance, but also has high resolution and definition of protein bands, and the separation speed and gelation speed of the colloid are faster than Laemmli gel. Although the traditional Laemmli colloid has excellent performance, it does not possess long-term preservation ability. Compared with the Laemmli colloid, the electrophoretic gel of the present invention can maintain a longer shelf life, and it can still maintain normal separation efficiency when stored at a constant temperature of 37 ° C for more than 12 days. Equivalent to more than one year at 4 °C.

Description

technical field [0001] The invention relates to an electrophoresis gel and a preparation method thereof, in particular to an SDS-polyacrylamide electrophoresis gel and a preparation method thereof. Background technique [0002] SDS-polyacrylamide gel electrophoresis is mainly used to separate proteins and oligonucleotides. Polyacrylamide gel (PAGE) has become the most commonly used support medium for biochemical experiments. The SDS-polyacrylamide gel electrophoresis technique established by using strong anionic SDS is mainly used to analyze proteins and peptides and determine their molecular weights. Laemmli of the United Kingdom formally created a method for separating proteins by denaturing polyacrylamide gel electrophoresis containing sodium dodecyl sulfate (SDS) in 1970. SDS-polyacrylamide gel electrophoresis requires polyacrylamide gel as a carrier for electrophoresis in experimental applications. [0003] Although traditional Laemmli gel has good separation performa...

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Problems solved by technology

[0003] Although the traditional Laemmli gel has good separation performance, it cannot be stored for a long time. The gel will be hydrolyzed during refrigeration, which will reduce the definition of protein bands and increase the difficulty of protein analysis.

At present, the large international prefabricated gel manufacturing companies (Pierce, Invitrogen, Bio-Rad, etc.) all have their own unique prefabricated gel patented production technology, but the electrophoresis formula is kept secret or subject to strict patent protection, while domestic The research on SDS-polyacrylamide gel electrophoresis precast gel has just started, and the exploration of colloid formula has just begun

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