Process for extracting and separating coenzyme Q10 from mushroom dregs

A coenzyme and bacterial residue technology, applied in the field of chemical engineering, can solve the problems of low silica gel reuse times, large consumption of solvents, silica gel, and organic solvents, and achieve parameters that are easy to control, waste liquid output, and low cost. cheap effect

Active Publication Date: 2016-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process can produce coenzyme Q10, but the consumption of organic solvents in the process is large, and the large amount of silica gel consumed in the chromatographic purification process is low in reuse times, seriously polluting the environment, and is an environmentally non-friendly process.
[0008] Therefore, the existing techniques for extracting and purifying coenzyme Q10 from fungus slag still have certain shortcomings. When applied to industrial production, there are problems such as large consumption of solvent and silica gel, and large pollution of waste water and solid waste.

Method used

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  • Process for extracting and separating coenzyme Q10 from mushroom dregs
  • Process for extracting and separating coenzyme Q10 from mushroom dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Weigh 70g of the fungus residue (wherein, the mass percentage of coenzyme Q10 is 2.6%), fill it into the percolation column (Φ2.0×35cm) by wet method, and the accumulated volume is about 100mL after the filling is uniform, and soak at a constant temperature of 10°C 2h, make it fully swell, open the outlet valve of the percolation column, and at the same time continuously add n-hexane from the top of the column, control the flow rate at 2.5-3.0mL / min, until the volume of the percolation liquid collected is 530mL.

[0044] Repeat several times to collect enough percolation liquid as the raw material liquid for subsequent operations. After analysis, the solid concentration is 4.7mg / mL, the content of coenzyme Q10 is 72.1%, and the extraction rate is 98.7%.

[0045] (2) Concentrate the percolation solution to remove the solvent, add n-hexane to dissolve, and prepare a solution with a solid concentration of 300 mg / mL. Take 20ml of the solution, and carry out three-stage ...

Embodiment 2

[0048] (1) The method of operation is the same as in Example 1.

[0049] (2) Concentrate the percolation solution to remove the solvent, add n-hexane to dissolve, and prepare a solution with a solid concentration of 300 mg / mL. Take 20ml of this solution, and use an equal amount of N,N-dimethylformamide as the extractant for five-stage countercurrent extraction. After the extraction balance, analyze the n-hexane phase of the raffinate, the content of coenzyme Q10 in the n-hexane phase after the five-stage extraction is 94.3%, and the solid concentration is 205mg / mL, the total recovery rate of coenzyme Q10 in the five-stage countercurrent extraction of this extraction process is 89.4% .

[0050] (3) Concentrate the extracted raffinate, add acetone at 30°C until it is completely dissolved, the solid-to-liquid ratio is 1:25, then gradually lower the temperature to 5°C, cool and crystallize for 24 hours, filter, and wash with an appropriate amount of cold ethanol. After fully dra...

Embodiment 3

[0052] (1) The method of operation is the same as in Example 1.

[0053] (2) Concentrate the percolation solution to remove the solvent, add n-hexane to dissolve, and prepare a solution with a solid concentration of 300 mg / mL. Take 20ml of this solution, use an equal amount of N,N-dimethylformamide as the extractant, and n-hexane as the detergent, and perform six-stage fractional extraction. The specific process is: Fractional distillation extraction is divided into three stages of extraction section and three stages of washing section. The first stage enters the fractionation extraction system, and the last stage of the washing section merges with the raw material liquid and enters the extraction section together. The extraction phase and the washing phase are subjected to multi-stage countercurrent extraction. The primary outflow is the raffinate rich in coenzyme Q10. Analysis of the raffinate showed that the content of coenzyme Q10 in the n-hexane phase after extraction w...

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Abstract

The invention discloses a process for extracting and preparing high-purity coenzyme Q10 from mushroom dregs. The mushroom dregs serve as raw materials to be subjected to percolation extraction, and a coenzyme Q10 percolation extracting solution is obtained; the coenzyme Q10 percolation extracting solution is subjected to multilevel extraction for purification, and raffinate is obtained; the raffinate is subjected to crystallization treatment, finally, the high-purity coenzyme Q10 with the purity reaching 98% or above is obtained, and the yield is 95% or above. The whole process is simple, reliable and easy to operate and achieve, and parameters are convenient to control.

Description

technical field [0001] The invention belongs to the technical field of chemical engineering, and in particular relates to a process flow for extracting high-purity coenzyme Q10 from fungus residue. Specifically, high-purity coenzyme Q10 is extracted and separated from fungus residue by using combined separation processes such as percolation extraction, extraction and impurity removal, and crystallization. Background technique [0002] Coenzyme Q compounds are fat-soluble quinone compounds that widely exist in nature. Coenzyme Q10 exists in the human body, and its standard name is 2,3-dimethoxy-5-methyl-6-decyl isopentenyl - Benzoquinone. Its structural formula is as follows: [0003] [0004] Coenzyme Q10 widely exists in the cells of organisms and has important physiological functions. Coenzyme Q10 is an essential component of energy metabolism, and its main function is to play the role of transporting protons and electrons in the respiration process, so as to generat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/10C07C50/28
CPCC07C46/10C07C50/28
Inventor 鲍宗必黄钰清任其龙杨亦文邢华斌杨启炜张治国苏宝根
Owner ZHEJIANG UNIV
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