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A strain of Ormer Kodak yeast degrading patulin and its application

A patulin and yeast technology, applied in the field of microorganisms, can solve the problems of unsatisfactory control effect of patulin and great influence of environmental factors, etc.

Active Publication Date: 2019-12-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control effect of antagonistic antibiotics on patulin is not ideal, and it is greatly affected by environmental factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: Omer Kodak yeast ( K. ohmeri ) Screening method for HYJM34

[0011] A total of 58 strains of yeasts collected from seabed silt, seawater and some marine organisms in the vast sea area of ​​China were preserved in this laboratory for cultivation. Inoculate 100 μL of activated yeast fermentation broth into 10 mL of YPD liquid medium containing 10 μg / mL of patulin (add hydrochloric acid to adjust the pH to 4.0), add 100 μL of sterile water to the blank, and culture on a shaking table at 28 °C. After 24 h, it was centrifuged at 4000 r / min for 10 min, and the content of patulin in the supernatant was determined by high performance liquid chromatography.

[0012] By comparing the degradation rate of patulin by various marine yeasts, a strain with strong degradation ability to patulin was screened out, and the degradation rate of patulin could reach 95.19% after 24 hours. According to the 26S rRNA sequence analysis, the strain was Saccharomyces americana.

Embodiment 2

[0013] Embodiment 2: Affect Omer Kodak yeast ( K. ohmeri ) HYJM34 degrading factors of patulin

[0014] For the preserved Omer Kodak yeast ( K. ohmeri ) HYJM34 were cultured, and the cells were collected by centrifugation (4000 r / min, 10 min, 4 ℃). (1) Resuspend the bacteria in 5 mL of citric acid-disodium hydrogen phosphate buffer (pH 4.0) containing 10 μg / mL of patulin, and adjust the concentration of the bacteria suspension to 1.0×10 8 CFU / mL. Cultivate at different temperatures (20°C, 25°C, 28°C, 30°C, 35°C, 40°C) at 100 r / min for 18 h; (2) Resuspend the bacteria in 5 mL of In citric acid-disodium hydrogen phosphate buffer (pH 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0), adjust the concentration of the bacterial suspension to 1.0×10 8 CFU / mL. Cultivate at 35°C and 100 r / min for 18 h; (3) Resuspend the bacteria in 5 mL of citric acid-disodium hydrogen phosphate buffer (pH 4.0) containing 10 μg / mL of patulin, and adjust the concentration of the bacteria suspension respective...

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PUM

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Abstract

The present invention belongs to the technical field of food biology, and discloses a strain of Kodomaea ohmeri HYJM34 capable of degrading patulin, wherein the preservation number is CGMCC No.9510. According to the present invention, the Kodomaea ohmeri HYJM34 provides a good patulin degradation effect, has a wide application range (the temperature of 25-35 DEG C, the pH value of 3-6, the bacterial suspension concentration of more than or equal to 5.0*10<7> CFU / mL, and the absence or presence of trace Cu<2+> and Zn<2+>), can be used for degradation of the patulin in fruit juices and other foods, and provides important significance for control of the patulin in food.

Description

technical field [0001] The invention involves a strain of Ormer Kodak yeast that degrades patulin, and it has been published on August 13, 2014 at No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, General Microbiology Center of China Microbiological Culture Collection Management Committee ( CGMCC), the deposit number is CGMCC No.9510, which belongs to the field of microorganisms. Background technique [0002] Patulin (4-hydroxy-4H-furo [3,2c] pyran-2[6H]-one) is a water-soluble lactone compound. As a secondary metabolite of a variety of fungi, its producing bacteria include Penicillium, Aspergillus, and Silkworm. Penicillium expanses is the main strain producing patulin, which can infect apples and cause them to rot. Patulin is widely found in various fruits and vegetables and their products, especially apples and their products. As a mycotoxin, patulin can cause a series of acute and chronic diseases and lesions at the cellular level, and is teratogenic,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23L5/20C12R1/645
Inventor 徐莹董晓妍孟祥红汪东风
Owner OCEAN UNIV OF CHINA