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A kind of oat acidophilus for degrading waste lubricating oil and its application

A technology of oat acidophilus and waste lubricating oil, applied in the field of microorganisms, can solve the problems of poor treatment effect and harsh treatment conditions, and achieve the effects of simple operation, no secondary pollution, and saving treatment costs.

Active Publication Date: 2019-04-09
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional physical and chemical methods have the disadvantages of harsh treatment conditions and poor treatment effects, while biological methods have the advantages of low cost, good treatment effects, no secondary pollution, and on-site treatment.

Method used

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  • A kind of oat acidophilus for degrading waste lubricating oil and its application
  • A kind of oat acidophilus for degrading waste lubricating oil and its application
  • A kind of oat acidophilus for degrading waste lubricating oil and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Study on the maximum degradation ability of bacteria strains to waste lubricating oil.

[0039]In order to study the maximum degradability of oat acidophilus for waste lubricating oil, the cell fermentation liquid was inoculated with 8% inoculum in 50 mL of inorganic salt medium (lubricating oil recovered from recycled oil treatment plant) containing different concentrations of lubricating oil The initial concentration of the oil is respectively 1102, 1208, 1304, 1402 mg / L), the initial pH value is adjusted to 6.20, and the temperature is 35 ° C. Shake flask experiments are carried out for the degradation of lubricating oil by oat acidophilus at 180 r / min. Monitor two indicators respectively: 1) Lubricant oil concentration in the sample liquid and calculate the lubricating oil degradation rate, the level of lubricating oil degradation rate represents the strength of the degradation ability of the bacteria at this concentration; 2) Measure the OD of the sample liquid 600...

Embodiment 2

[0042] Research on the ability of strains to degrade waste hydraulic oil.

[0043] In order to study the ability of oat acidophilus to degrade waste hydraulic oil, the cell fermentation liquid was inoculated into 50 mL inorganic salt medium containing waste hydraulic oil with different concentrations (the actual concentration of waste hydraulic oil was 106 mg / L, 204mg / L, 412mg / L, 608mg / L, 814mg / L, 1016mg / L, 1204mg / L, 1308mg / L), the initial pH value was adjusted to 6.20, the temperature was 35°C, and it was shaken at 180r / min Cultivate and carry out the shake flask experiment of oat acidophilus to degrade waste hydraulic oil, and monitor two indicators respectively: 1) the lubricating oil concentration in the sample liquid, 2) the OD of the sample liquid 600nm value. Figure 2-3 It reflects the situation of oat acidophilus degrading waste hydraulic oil at different initial concentrations, corresponding to the time required for complete degradation at the above-mentioned diffe...

Embodiment 3

[0045] Research on the ability of strains to degrade waste engine oil.

[0046] In order to study the ability of oat acidophilus to degrade waste engine oil, the cell fermentation liquid was inoculated into 50 mL inorganic salt medium containing different concentrations of waste engine oil (the actual concentrations of waste engine oil were 794 mg / L and 989 mg / L respectively) with an inoculum size of 8%. L, 1105mg / L, 1193mg / L), the initial pH value was adjusted to 6.20, the temperature was 35°C, and shaking culture was carried out at 180r / min to carry out the shaking flask experiment of oat acidophilus degrading waste hydraulic oil, and two indicators were monitored respectively. : 1) lubricating oil concentration in the sample liquid, 2) OD of the sample liquid 600nm value. Figure 4-5 It reflects the degradation of different initial concentrations of waste engine oil by oat acidophilus. The maximum concentration of oat acidophilus that can completely degrade waste engine oi...

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Abstract

The invention provides an oat acidophilus which can effectively degrade waste lubricating oil and its use. The oat acidophilus was preserved in the Guangdong Microbial Culture Collection Center on August 9, 2016, and the preservation number is GDMCC No: 60061. The strain has a significant degradation effect on high-concentration waste lubricating oil, waste engine oil, waste transformer oil, and waste hydraulic oil. The maximum concentration of degradable waste lubricating oil is 1304mg / L. The maximum concentration of transformer oil is 1204mg / L, 1105mg / L, 812mg / L respectively. The strain can also be applied to the biological degreasing treatment of the oleic acid residue produced in the regeneration process of waste lubricating oil; the acid residue with an initial oil content of about 50-80mg / g, after sowing the fermentation liquid of the oat acidophilus After 2.5-3.5 months of treatment at about 35-37°C, the oil content can be reduced to less than 1mg / g.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an oat acidophilus which can effectively degrade waste lubricating oil and its application. Background technique [0002] With the further acceleration of the industrialization process, the demand for lubricating oil and the production of waste lubricating oil continue to increase. In view of the global energy shortage and strict environmental protection requirements, waste lubricating oil must be regenerated and reused, which has given birth to the vigorous development of the waste lubricating oil regeneration and disposal industry. The regeneration and disposal of waste lubricating oil has experienced the development of technical processes such as sulfuric acid-clay, atmospheric and vacuum distillation, solvent refining, molecular distillation, etc. These processes inevitably produce industrial waste residues. The waste residue contains unreacted acidic subs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01
CPCA62D3/02A62D2101/20C12N1/205C12R2001/01
Inventor 姜岩张贤明
Owner CHONGQING TECH & BUSINESS UNIV