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Method for quantitatively detecting biological marker by utilizing glucose meter

A biological marker and quantitative technology, applied in biological testing, material inspection products, etc., can solve the problems of high cost, long time, not suitable for rapid detection and identification of biological markers, etc., and achieve low cost and short time consuming. Effect

Inactive Publication Date: 2016-11-23
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The detection methods of biomarkers include electrophoresis, radioactive analysis, traditional fluorescence spectroscopy, immunoassay, chromatography, etc. In recent years, many PCR-based molecular biology detection methods have emerged, although these methods are more advanced than traditional methods. Good accuracy and high sensitivity, but these detection methods require the use of laboratory-specific equipment and professional technicians for detection operations, which is time-consuming and costly
Therefore, it is not suitable for the rapid detection and identification of biomarkers

Method used

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  • Method for quantitatively detecting biological marker by utilizing glucose meter
  • Method for quantitatively detecting biological marker by utilizing glucose meter
  • Method for quantitatively detecting biological marker by utilizing glucose meter

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preparation example Construction

[0084] In the present invention, the preparation method of the recognition reagent linked with sucrase in the step B) comprises the following steps:

[0085] The sucrase and the coupling agent are mixed and connected for 30 to 60 minutes to obtain the coupling agent-sucrase complex;

[0086] Mix the thiol-modified recognition reagent, PB buffer and tris(2-carboxyethyl)phosphine, and let stand for 30-60 minutes to obtain the activated recognition reagent;

[0087] The coupling agent-sucrase complex is mixed with the activated recognition reagent and left to stand for 24-48 hours to obtain the recognition reagent linked with the sucrase.

[0088] In the present invention, the reaction temperature for the mixing and connection of the sucrase and the coupling agent is preferably 20-30° C., and the mixing and connection is preferably performed at a rotation speed of 20-30 r / min.

[0089] In the present invention, the preparation method of the recognition reagent connected with suc...

Embodiment 1

[0095] Link 100 μL of activated carboxyl magnetic beads with porcine circovirus type 2 PCV2 monoclonal antibody to obtain carboxyl magnetic beads with monoclonal antibody, then perform magnetic separation and wash 4 times with Buffer A solution. After magnetic separation, disperse the carboxyl magnetic bead precipitate in 100 μL of Buffer A solution containing PCV2 capsid protein—cap protein, and then mix it for 2 hours with a rotary mixer at 25°C and 25 r / min, and then perform magnetic separation of carboxyl magnetic beads. Beads were washed 3 times with Buffer A solution. Add 100 μL 1mg / L biotinylated PCV2 polyclonal antibody (dissolved in Buffer A solution) to the precipitation of carboxyl magnetic beads, then mix at 28°C for 45 minutes, magnetically separate carboxyl magnetic beads, wash 4 times with Buffer A solution, and carboxyl magnetic The bead precipitate was dispersed in 80 μL of 2 μM streptavidin (dissolved in Buffer A solution), and mixed for 50 min. Magnetically...

Embodiment 2

[0097] Link 60 μL of activated carboxyl magnetic beads to porcine circovirus type 2 PCV2 monoclonal antibody to obtain activated carboxyl magnetic beads, then perform magnetic separation and wash 3 times with Buffer A solution. After magnetic separation, disperse the carboxyl magnetic bead pellet in 100 μL of Buffer A solution (containing 25% pig serum) containing PCV2 capsid protein—cap protein, and then mix it with a rotary mixer at 28°C and 24r / min. 2h, then carry out magnetic separation of carboxyl magnetic beads, wash 3 times with Buffer A solution. Add 55 μL 1mg / L biotinylated PCV2 polyclonal antibody (dissolved in Buffer A solution) to the precipitation of carboxyl magnetic beads, then mix at 28°C for 55 min, magnetically separate carboxyl magnetic beads, wash twice with Buffer A solution, and remove the magnetic beads The precipitate was dispersed in 60 μL of 2 μM streptavidin (dissolved in Buffer A solution), and mixed for 45 minutes. Magnetically separate the carbox...

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Abstract

The invention provides a method for quantitatively detecting a biological marker by utilizing a glucose meter, aiming at realizing a non-disease diagnosis aim. The method comprises the following steps: (1) capturing the biological marker, wherein a capturing method comprises a competitive capturing method or a non-competitive capturing method; (2) converting a biological marker signal captured in the step (1) into a glucose signal; and (3) detecting the converted glucose signal by utilizing the glucose meter. The biological marker signal is converted into the glucose signal and the glucose meter is used as a detecting instrument so that the target biological marker can be simply, rapidly and quantitatively detected; and a result can be digitally output through the glucose meter, the consumed time is short and the cost is low.

Description

technical field [0001] The invention relates to the field of biomolecular detection, in particular to a method for quantitatively detecting biomarkers using a blood glucose meter. Background technique [0002] The detection methods of biomarkers include electrophoresis, radioactive analysis, traditional fluorescence spectroscopy, immunoassay, chromatography, etc. In recent years, many PCR-based molecular biology detection methods have emerged, although these methods are more advanced than traditional methods. Good accuracy and high sensitivity, but these detection methods require the use of laboratory-specific equipment and professional technicians for detection operations, which is time-consuming and costly. Therefore, it is not suitable for the rapid detection and identification of biomarkers. Contents of the invention [0003] In view of this, the purpose of the present invention is to provide a quick and easy method for detecting biomarkers. [0004] In order to achi...

Claims

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Application Information

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IPC IPC(8): G01N33/48
CPCG01N33/48
Inventor 刘斐张艳
Owner NANJING AGRICULTURAL UNIVERSITY
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