Enzymatic hydrolysis method of citrus pulp

A technology of citrus residue and enzymolysis, applied in application, animal feed, animal feed, etc., can solve the problems of restricting the release of nutrients, unfavorable digestion and absorption of animals, etc., and achieves increased feeding value, obvious changes in nutrients, and fiber structure. Significant change

Inactive Publication Date: 2016-12-07
CHONGQING ACAD OF ANIMAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, citrus pomace (air-dried basis) contains a large amount of fibrous substances, wherein the pectin content is as high as 42.25%, the cellulose content is 15.95%, and the hemicellulose content is 10.06% (Brock Lundberg, 2014). Formed into a network structure, which limits the release of other nutrients and is not conducive to animal digestion and absorption

Method used

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  • Enzymatic hydrolysis method of citrus pulp
  • Enzymatic hydrolysis method of citrus pulp
  • Enzymatic hydrolysis method of citrus pulp

Examples

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Effect test

Embodiment 1

[0024] An enzymatic hydrolysis method of citrus pomace, the method steps are as follows:

[0025] Dry the citrus residues and crush them, pass through a 20-mesh sieve, take an appropriate amount of citrus residues and sterilize them in an autoclave at 121°C for 30 minutes, and fill the sterile citrus residues into 150mL conical flasks as an enzymatic hydrolysis matrix. Weigh 30g, add sterile water at a ratio of 1:1.5 (m / v), mix, and then add 0.2% combined enzyme solution, mix well, seal the Erlenmeyer flask and place it in a constant temperature water bath Carry out enzymolysis reaction in the medium, enzymolysis 18-24h under the condition of temperature of 30~50℃, lyophilize, rehydrate for 24h, pulverize, measure true protein, crude fiber, total reducing sugar content. The composition of the combined enzyme liquid is: pectinase + cellulase + glucanase, the mass ratio of the three is 2:1:1.

Embodiment 2

[0027] An enzymatic hydrolysis method of citrus pomace, the method steps are as follows:

[0028] Dry the citrus residues and crush them, pass through a 20-mesh sieve, take an appropriate amount of citrus residues and sterilize them in an autoclave at 121°C for 30 minutes, and fill the sterile citrus residues into 150mL conical flasks as an enzymatic hydrolysis matrix. Weigh 30g, add sterile water at a ratio of 1:1.5 (m / v), mix, and then add 0.2% combined enzyme solution, mix well, seal the Erlenmeyer flask and place it in a constant temperature water bath Carry out enzymolysis reaction in the medium, enzymolysis 18-24h under the condition of temperature of 30~50℃, lyophilize, rehydrate for 24h, pulverize, measure true protein, crude fiber, total reducing sugar content. The composition of the combined enzyme liquid is: pectinase + cellulase + mannanase, the mass ratio of the three is 2:1:1.

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Abstract

The invention discloses an enzymatic hydrolysis method of citrus pulp. The enzymatic hydrolysis method of the citrus pulp comprises the following steps: mixing the citrus pulp with an enzyme preparation, and then, carrying out enzymatic hydrolysis at a temperature of 30-50 DEG C for 18-24 hours; the enzyme preparation comprises the following components: one selected from dextranase and mannose, pectinase and cellulose; and the enzyme preparation preferably comprises the following components: pectinase, cellulose and dextranase. The enzyme preparation compound utilized in the enzymatic hydrolysis method is capable of obviously changing the fiber structure of the citrus pulp after the enzymatic hydrolysis, so that the coarse fiber content is reduced by about 5%; moreover, the true protein content is increased by 10% or higher, and the reduced sugar content is increased by about 23%. Thus, the nutrient components are obviously changed, and the feeding values are increased.

Description

technical field [0001] The invention belongs to the technical field of biomass application, and in particular relates to an enzymatic hydrolysis method of citrus residue. Background technique [0002] my country is a big country of citrus production. In 2014, the output of citrus in my country was 34.93 million tons, and the area of ​​citrus orchards was 25.21 thousand hectares. Citrus pomace is a by-product of tangerine fruit after being squeezed or canned, accounting for about 25% to 50% of the fruit weight, and the output is huge. However, due to the high moisture content of citrus fresh residue, which can reach more than 80%, it is not conducive to storage and transportation. Except for a very small part used to extract pectin, dietary fiber and other substances, the usual treatment method is to stack it in the open air and landfill it. , used as fertilizer, or discarded in the form of waste or discharged into rivers, seriously affecting the ecological environment and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14
Inventor 刘志云周晓容姚焰础杨飞云黄金秀黄健
Owner CHONGQING ACAD OF ANIMAL SCI
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