Separation and purification method of agglutinin from alocasia cucullata

A technology for separation and purification of lectins, which is applied in the field of separation and purification of lectins from the taro taro, which can solve the problems of high production and use costs, complex process, and low efficiency of lectin separation and purification

Inactive Publication Date: 2016-12-07
SOUTHERN MEDICAL UNIVERSITY
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Problems solved by technology

[0005] The present invention provides a method for purifying lectin from Taro, which aims to solve the complicated technical process of separating and purifying lectin fr...

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  • Separation and purification method of agglutinin from alocasia cucullata

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Embodiment Construction

[0016] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the invention.

[0017] figure 1 It shows the implementation process of the method for the separation and purification of the taro lectin provided by the embodiment of the present invention.

[0018] The method includes the following steps:

[0019] In step S101, the taro powder is soaked in phosphate buffer solution for 1 hour, ultrasonically treated for 10 minutes, filtered to obtain the filtrate, and centrifuged to collect the supernatant;

[0020] In step S102, solid ammonium sulfate was added to the supernatant until the saturation reached 90%, so that the protein in the supernatant was precipitated, and the pre...

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Abstract

The invention relates to a separation and purification method of agglutinin from alocasia cucullata. The separation and purification method specifically comprises the following steps of soaking coarse powder of the alocasia cucullata in a phosphate buffer solution for 1h, performing 240W ultrasonic processing for 10min, performing filtration, taking a filtrate, performing centrifugation, and collecting a supernatant; compounding the supernatant into a 90% ammonium sulphate solution to precipitate protein, performing centrifugation, and taking precipitation; performing dialysis, performing desalting, performing freeze drying, enabling the dissolved supernatant to pass through a DEAE-52 ion-exchange chromatographic column, collecting third elution peaks, detecting blood coagulation activity, performing dialysis, performing desalting, and performing freeze drying; and enabling the dissolved supernatant to pass through a Sephadex G-75 column, collecting first elution peaks, detecting blood coagulation activity, and performing freeze drying. The technological course of separating and purifying the agglutinin from the alocasia cucullata is simple and convenient, the separation and purification efficiency of the agglutinin is high, the production cost and the use cost are low, the practicality is high, and the separation and purification method has high popularization values and high application values.

Description

technical field [0001] The invention belongs to a method for separating and purifying protein products, in particular to a method for separating and purifying taro lectin. Background technique [0002] Lectins are multivalent, carbohydrate-binding proteins of non-immune origin. It selectively binds sugar chains on the cell surface, leading to cell aggregation. Although the physiological functions of lectins have yet to be elucidated, lectins have been widely used in biochemistry, immunology, histochemistry, It has played a wide range of roles in clinical diagnosis and treatment. [0003] Taro is a perennial evergreen herb growing in southern my country. In Chinese herbal medicine, chamaema chamaema is often used to reduce swelling and stagnation. Hou Xuewen et al. isolated a lectin AEL from this plant, and found that the conversion rate of AEL to cultured human peripheral blood leukocytes was as high as 88%, which was higher than that of the control PHA, and it was helpf...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/18
Inventor 蔡红兵黄嘉维李欣郝亚娟
Owner SOUTHERN MEDICAL UNIVERSITY
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