Method for accelerating synthesis of L-arginine through enhancing ammonia assimilation

An assimilation, arginine technology, applied in microorganism-based methods, biochemical equipment and methods, nucleic acid carriers, etc., can solve problems such as no relevant research reports

Active Publication Date: 2016-12-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the metabolic engineering of microbial fermentation L-arginine-producing strains has mainly focused on the optimization of carbon metabolism flow and cofactor supply optimization, while there is no relevant research report on cellular ammonia assimilation related to nitrogen atom supply.

Method used

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  • Method for accelerating synthesis of L-arginine through enhancing ammonia assimilation
  • Method for accelerating synthesis of L-arginine through enhancing ammonia assimilation
  • Method for accelerating synthesis of L-arginine through enhancing ammonia assimilation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Effects of individual expression of glutamate dehydrogenase, glutamine synthase and aspartase on L-arginine synthesis

[0025] 403 / pDXW-10-gdh, 403 / pDXW-10-glnA and 403 / pDXW-10-aspA were fermented in shake flasks, and the composition and fermentation conditions of the fermentation medium were the same as those in the control example. Fermentation 60h L-arginine yield 403 / pDXW-10-gdh was 15.7g / L, 403 / pDXW-10-glnA was 17.5g / L, 403 / pDXW-10-aspA was 18.1g / L.

Embodiment 2

[0026] Example 2: Effect of co-expression of glutamine synthase and aspartase on L-arginine synthesis

[0027] It can be seen from Example 1 that overexpression of glutamine synthase and aspartase alone can significantly increase the production of C. crenatumSDNN403 L-arginine, while overexpression of glutamate dehydrogenase L-arginine production does not increase significantly . This may be because the original glutamate dehydrogenase activity of C. crenatum SDNN403 can meet the needs of L-arginine synthesis. Therefore, the effect of co-expression of glutamine synthase and aspartase on L-arginine synthesis was continued. 403 / pDXW-10-glnA-aspA shake flask fermentation, the fermentation medium composition and fermentation conditions are the same as those in the control example. The yield of L-arginine was 19.3g / L after 60h fermentation.

Embodiment 3

[0028] Example 3: Effect of co-expression of glutamine synthase, aspartase and glutamate dehydrogenase on L-arginine synthesis

[0029] It can be seen from Example 2 that excessive co-expression of glutamine synthase and aspartase can further increase the L-arginine production of C. crenatum SDNN403. 403 / pDXW-10-glnA-aspA L-arginine synthesis ability was significantly improved, then L-glutamic acid was used as the direct precursor of L-arginine synthesis, and L-glutamic acid in strain 403 / pDXW-10-glnA-aspA Amino acid supply has the potential to be the limiting factor for L-arginine synthesis. Therefore, the effect of co-expression of glutamine synthase, aspartase and glutamate dehydrogenase on L-arginine synthesis was continued. 403 / pDXW-10-glnA-aspA-gdh shake flask fermentation, fermentation medium composition and fermentation conditions are the same as the control example. The L-arginine yield was 20.8g / L after 60h fermentation.

[0030]

[0031]

[0032]

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PUM

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Abstract

The invention provides a method for accelerating the synthesis of corynebacterium crenatum L-arginine through enhancing ammonia assimilation. Specifically, the yield of the corynebacterium crenatum SDNN403 L-arginine can be effectively improved through excessive co-expression of a glutamine synthase coding gene glnA and a glutamate dehydrogenase coding gene gdh of a key enzyme corynebacterium crenatum source of an ammonia assimilation way, and an aspartase coding gene aspA of an escherichia coli source in the corynebacterium crenatum SDNN403.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation production of amino acids, in particular to a method for promoting L-arginine synthesis by enhancing ammonia assimilation. technical background [0002] L-arginine is a semi-essential amino acid in humans and animals. It is the synthetic precursor of various biologically active substances and has various unique physiological and pharmacological effects. With the continuous in-depth study and understanding of the biological function of arginine, the use of arginine in medicine, food and feed industries is becoming more and more extensive. [0003] The production methods of L-arginine include hydrolysis method and fermentation method. Most of the existing manufacturers in my country still mainly use the protein hydrolysis method to produce L-arginine. This method has serious environmental pollution and low yield, and is not suitable for large-scale production. The fermentation proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/10C12N15/77C12R1/15
CPCC12N9/0016C12N9/88C12N9/93C12N15/77C12N2800/101C12P13/10C12Y104/01002C12Y104/01003C12Y104/01004C12Y403/01001C12Y603/01002
Inventor 饶志明满在伟徐美娟杨套伟张显
Owner JIANGNAN UNIV
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