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Primer for detecting vitality of strawberry seedlings and application of the primer

A technology of viability and strawberry, applied in the field of plant biology, can solve the problem of inaccurate judgment of vigor of strawberry seedlings, achieve beneficial evaluation, high repeatability, and reduce workload

Active Publication Date: 2016-12-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a primer for detecting the viability of strawberry seedlings, so as to solve the problem of inaccurate judgment of strawberry seedling viability in the prior art

Method used

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  • Primer for detecting vitality of strawberry seedlings and application of the primer
  • Primer for detecting vitality of strawberry seedlings and application of the primer
  • Primer for detecting vitality of strawberry seedlings and application of the primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Sampling of experimental materials: 'Toyonoka' strawberry seedlings were collected from Jiangsu Academy of Agricultural Sciences. Put the strawberry seedlings in good growth condition in the greenhouse, and plant 20 plants in pots respectively after 0h, 6h, 12h, 18h, 24h, 30h, 36h, and 48h, and take sample leaves and stolons at the same time, and freeze them in liquid nitrogen , stored in a -80°C ultra-low temperature freezer. After 10 days, the survival rate of each treated seedling was counted.

[0043] 2. DNA extraction from strawberry seedlings: Genomic DNA was extracted by the improved CTAB method, and the specific operation was as follows: fresh leaves (0.1 g) were crushed in liquid nitrogen, and quickly transferred into a 1.5 mL centrifuge tube. Add 600 μL of pre-warmed CTAB (2% CTAB: 100 mM Tris-HCl, pH 8.0; 20 mM EDTA, pH 8.0; 1.4 M NaCl; add 0.4% β-mercaptoethanol before use) extraction buffer. Incubate at 65°C for 30 minutes, and invert several times dur...

Embodiment 2

[0058] Toyonoka ('Toyonoka') strawberry seedlings in good growth condition were placed in a greenhouse without any protective measures, and allowed to wilt naturally, and then respectively after 0h, 6h, 12h, 18h, 24h, 30h, 36h, and 48h, Get 20 plants and plant them in pots, water them, and count the survival rates of strawberry seedlings in each treatment period after planting in suitable environmental conditions for 10 days.

[0059] Then compared with the Ct values ​​obtained by the fluorescent quantitative PCR of GAPDH1 and TIM1 genes in Example 1 corresponding to each time period (0h, 6h, 12h, 18h, 24h, 30h, 36h, 48h). The results are shown in Table 3.

[0060] Table 3 The relationship between the Ct values ​​of GAPDH1 and TIM1 genes and the vigor of strawberry

[0061] GAPDH1 gene Ct value

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Abstract

The invention discloses a primer for detecting vitality of strawberry seedlings. The primer comprises the following primer pairs: 1) a primer pair for detecting GAPDH1 gene, of which the nucleotide sequences are represented as the SEQ ID No.3-4; and 2) a primer pair for detecting TIM1 gene, of which the nucleotide sequences are represented as the SEQ ID No.5-6. The invention also discloses a method of detecting the vitality of strawberry seedlings with the primer. The invention provides a molecular detection method for the vitality of the strawberry seedlings, which is quick and simple, is strong in specificity, and is accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of plant biology, and in particular relates to a primer for detecting the viability of strawberry seedlings and an application thereof. Background technique [0002] Strawberry (Fragaria×ananassa Duch.) is a perennial evergreen herbaceous plant of the Rosaceae (Rosaceae) genus Fragaria. The fruit is tender and juicy, bright in color, pleasant in aroma, and moderately sweet and sour in taste. It is one of the traditional excellent berry fruits in my country. Strawberry fruit is rich in vitamin C, organic acids, sugar, minerals and pectin and other nutrients. In recent years, studies have found that biologically active substances such as ellagitannins, anthocyanins and proanthocyanidins contained in strawberries can prevent the occurrence of cardiovascular and cerebrovascular diseases and cancer, so strawberries are favored by consumers. [0003] Seedling viability refers to the ability of seedlings to survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 朱旭东房经贵贾海锋王保菊
Owner NANJING AGRICULTURAL UNIVERSITY