Method for repairing lead polluted soil by using EDTA and brassinolide co-induced rye grass
A technology of lead-contaminated soil and brassinolide, applied in the restoration of polluted soil and other directions, can solve the problems of yellowing growth rate of leaves, low biomass, dwarf plants, etc., and achieves alleviation of toxicity, high restoration efficiency, and restoration short cycle effect
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Embodiment 1
[0027] 1 test plant
[0028] Use perennial ryegrass.
[0029] 2 test soil
[0030] The soil for testing comes from vegetable fields around Changzhou Science and Education City. Its basic physical and chemical properties (background value) are: pH value is 7.2, organic matter content is 7.48g / kg, available nitrogen, available phosphorus, available potassium and soil total Pb content are respectively: 35.54, 24.77, 121.42 and 30.62 mg / kg.
[0031] 3 pot experiments
[0032] heavy metal Pb to analyze pure Pb(NO 3 ) 2 The solution was sprayed on the soil, fully mixed by a mixer, and equilibrated for 6 months to simulate the Pb-contaminated soil in the mining area (Pb content is 500mg / kg), and the original clean soil was used as a control. Each plastic pot 25cm (diameter) × 17cm (height) is filled with 2.5kg of soil. Seeds were sterilized with 4% NaClO for 30 minutes, and cleaned with deionized water before sowing, 250 seeds per pot (to ensure more than 150 plants per pot aft...
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