49 results about "Myrmica lonae" patented technology

The invention provides a perennial ryegrass breeding method based on a CRISPR / Cas system. A CRISPR / Cas genome-editing technique is applied to breed improvement of perennial ryegrass, gene site-directed knock-out is conducted on lignin synthesized key enzyme genes LpCCR and LpCOMT, homozygous and transformation carrier deleted mutants are selected out, the breeding material of the perennial ryegrass of which the lignin content is decreased is bred, and important significance for breeding research and breed improvement of pasture is achieved.

The invention is concerned with the application method that uses the straw and the lawn plant to recover the housing refuse with heavy metal, it is: the 10 to 80 of housing refuse compost mix with 1 to 10 of straw to make the lawn plant culture substrate, seeds on the lawn plant culture substrate and give water every day, uses the ICP-AES method to test the heavy metal content after distilling by the lawn plant from cradle the upside of the lawn plant; the result shows with the paddy straw, the recover actuation of the Cr, Ni and Cd by the secular-grow ryegrass, and the recover actuation of the Cr and Ni by festuca arundinacea are big foreground.

The invention relates to a production method for trampling-resistant soilless lawn matrix. The method comprises the following steps of: (1) selecting cool season lawn grass as seeds; (2) mixing slag and organic sludge compost in a ratio to form a matrix growth layer; (3) mixing mushroom production leftovers, saw dust, straw powder and industrial urea formaldehyde foam particles in a ratio to form an elastic material layer; (4) spreading a plastic mulching film serving as an isolation layer on the ground, and spreading a polypropylene fiber sunshade net serving as a turf bottom layer on the isolation layer; (5) sequentially spreading the matrix growth layer and the elastic material layer on the turf bottom layer; (6) sowing meadow grass and perennial ryegrass on the elastic material layer to form a seed layer, and covering and lightly pressing the seed layer by using a special non-woven fabric after sowing to form a bud protection layer so as to finish constructing a lawn bed; (7) controlling the growth environment of the lawn bed of the lawn; and (8) after the lawn is formed, performing trampling treatment by adopting an artificial trampling method. The trampling-resistant soilless lawn matrix has the characteristics of good recovery growth performance after the lawn is trampled, low cost and good water holding property.

The invention discloses a method for establishing a high-frequent regenerating system for the perennial ryegrass, providing good receptor material for the work of genetic conversion, comprising: sterilizing the seeds, inducing the embryo callus on the traumatic inducing culture medium by employing the mature embryo as explant, shifting it to the regenerative culture medium after subculture for the regenerative sprout growth, then shifting it to the root-growing culture medium for the root growth and replanting it after getting the complete plant. The draw material of explant is easy and free of seasonal constrain, the sterilizing mode is safe and effective, by regulating the concentration of the hormone and addictive in the traumatic inducing culture medium, growing culture medium and root-growing culture medium, the inductivity of embryo callus is increased effectively, and the callus embryo structure is kept in the course of subculture; the regenerative rate and root-growing rate is very high and the survival rate of replanting is 100%.

The invention provides a method for substituting for flaveria bidentis by utilizing combination of sunflower and perennial ryegrass; in the method, the combination of the sunflower and the perennial ryegrass is adopted to biologically substitute for the flaveria bidentis; the germination rate of the flaveria bidentis can be reduced by 51.52 percent, the plant height can be reduced by 77.71 percent, the branching quantity of the single plant is reduced by 98.23 percent averagely, the leaf quantity of the single plant is reduced by 97.57 percent averagely, the biomass liveweight of the single plant is reduced by 97.87percent averagely, and the average soluble protein content is reduced by 73.5 percent, and the method has remarkable control function to external invasion of the flaveria bidentis; the seeds of the perennial ryegrass and the sunflower are obtained easily, and the planting method is simple and is easy to operate; in addition, the perennial ryegrass is a good pasture, the sunflower is an important oil crop; by adopting method, while controlling the quantity of the flaveria bidentis, a certain of economic value can be realized.

The invention provides a method for biologically repairing soil polluted by petroleum hydrocarbon compounds. The method comprises the steps of planting repairing plants in the soil and applying repairing biological agents to the soil. The repairing plants are compositions of one or more of annual ryegrass, perennial ryegrass, tall fescue grass and prairie gramineous crops. The repairing biological agents at least comprise bacterium solutions containing plant promoting bacteria, and the bacterium solutions contain plant promoting bacteria Pseudomonas cedrina UW3 and Pseudomonas putida UW4. With the method, time of soil repairing can be greatly shortened, and generally in the seriously-polluted area, the soil can be repaired to the level before pollution within three years to five years.

The present invention discloses meat chicken raising method of artificial planting grass and free raising. The artificial grass land is planted in grass land and through reclamation, land preparation, applying fertilizer, seeding white clover, Lotus japonicus, perennial ryegrass and dayflower, weeding and other measures. After the grass grows to cover 90 % over land and height over 20 cm, meat chicken may be raised freely in the pasture. The present invention has the advantages of low feed cost, high chicken quality, less diseases, good taste of chicken, no problem of disposing chicken manure, etc. and is favorable to maintaining soil and water and protecting environment.

The invention discloses an ensiling composition containing perennial ryegrass and vicia sativa and an application of ensiling composition. The ensiling composition is prepared from following raw materials according to fresh weights in parts by weight: 65-80 parts of perennial ryegrass, 20-35 parts of vicia sativa, a cellulase preparation and a lactic acid bacteria composite additive; the lactic acid bacteria composite additive is composed of plant lactobacillus, bacillus subtilis and glucose. Based on the properties of the perennial ryegrass and the vicia sativa, specific fermentation accelerants are selected from a plurality of fermentation accelerants to be combined to obviously improve the fermentation quality of a mixed ensiling feed; the ensiling composition is low in cost, safe and reliable and is easy to use.

The invention discloses a stereo persimmon-pasture-livestock allocation standard production technology for Weibei dryland. The production technology is characterized by including the steps: (1) mixing orchardgrass, perennial ryegrass, trifolium repens and onobrychis according to a proportion of 2:4:2:2; (2) spreading 15kg calcium superphosphate serving as base fertilizer; (3) adopting a line spacing seeding mode for spring seeding or autumn seeding of the mixed seeds in an apple orchard with the planting density (row spacing X row spacing) being 2.5mX4m, wherein 2.0-2.5kg seeds are sown per mu; (4) cradling and grazing. Adopting the production technology can realize secondary development and utilization of land, save grain cost for livestock feeding and increase economic income; seeding in persimmon orchards can improve organic substances content in soil, reduce diseases and insect pests and improve fruit yield and quality.

The invention belongs to the field of environmental protection technique for the room greening and decorating. The invention employs the technique and principle of the cultivation without soil, which comprises: applying certain base material without soil (dry saw powder and rice husk) as culture medium, putting the culture medium into the collar basal disc (0.05 m2) which is specially-produced and suitable for placing in room, choosing cold-season type lawn straw which is liable for root growing (high fescue, Kentucky bluegrass and perennial ryegrass) with high quality, spraying the combined nutrient solution with concentration of 0.3% for 500 ml, applying series farming curing measures, making the root of the straw grow transversely in a short period for the forming of firm and dense straw plate which is facilitating for cutting, shaping setting, transportation and industrial production. The method is characterized by the following features when compared with the existing ones: suitable for room placing and rapid planting, short time for lawn forming, the base material is easy to get, saving labor, low administrative expense, free of weed, light weight, high quality of the lawn, the ability to moderate the room climate and absorb the room pollution.

The invention provides a cultivation method for lawns of a sloping field type coastal heavy saline-alkali land. The cultivation method comprises the following steps of laying a salt discharge system, laying an irrigation system, carrying out raw soil preparation, planting festuca arundinacea, planting zoysia matrella, planting perennial ryegrass and carrying out planting post-management. The step of laying the salt discharge system comprises the following steps: digging salt discharge ditches properly along the altitude direction of a sloping field from the top of the sloping field to the base part of the sloping field, wherein the salt discharge ditches are 1m to 1.2m in depth; and laying salt discharge underground pipes in the ditches according to a principle that one pipe is arranged in one ditch. The step of laying the irrigation system comprises the following steps: laying the irrigation system at a position which is 75cm to 80cm in depth below the ground surface, wherein the laying space of irrigation water pipes is 5m; setting up one water spraying pipe every other 5m on each water pipe, wherein each water spraying pipe can extend to be a part which is 15cm to 20cm above the ground surface and a spraying nozzle capable of realizing 360-degree water spraying is mounted at a pipe opening of each water spraying pipe. The cultivation method for the lawns of the saline-alkali land, provided by the invention, has the advantages of rapid lawn formation speed, high lawn survival rate and uneasiness of degradation.

The invention discloses a wheat straw and perennial ryegrass containing silage composition and application thereof. The wheat straw and perennial ryegrass containing silage composition consists of the following components in parts by fresh weight: 50 to 80 parts of wheat straws, 20 to 50 parts of perennial ryegrass, a cellulase preparation and a lactobacillus composite additive, wherein the lactobacillus composite additive consists of lactobacillus plantarum, bacillus subtilis and glucose. According to the wheat straw and perennial ryegrass containing silage composition and the application thereof, a specific fermentation promoter combination is selected from a plurality of fermentation promoters according to the characteristics of the wheat straws and the perennial ryegrass, so that the fermentation quality of mixed silage feed is remarkably improved, and the wheat straw and perennial ryegrass containing silage composition is low in cost, safe, reliable and easy to use.

The invention relates to a method for planting forage grass on a sunward side of hillside land with the slope greater than 15 degrees in an alpine mountainous area. The method comprises the steps thatthe forage grass is planted in a rotation place in the alpine mountainous area with the altitude of 2,000-2,500 m in the southwestern Yunnan province of China, the sunward-side hillside land with theslope greater than 15 degrees is selected as the rotation place, and after former crops are harvested, the forage grass is planted; forage grass planting is conducted by adopting the mode that 9-11%of annual ryegrass seeds, 18-22% of perennial ryegrass seeds, 9-11% of hybrid ryegrass seeds, 28-32% of dactylis glomerata seeds, 28-32% of trifolium repens seeds and base fertilizer are mixed and sowed, and grazing can be conducted when the forage grass grows till the height of a grass layer exceeds 5 cm; when the forage grass is surplus, the harvested forage grass is dried and prepared into green hay or silage, and the stubble height during grass harvesting is 5 cm. The method for planting the forage grass in the alpine mountainous area has the advantages that the method is suitable for being implemented in the southwestern Yunnan province of China, and the forage grass is quick in propagation, good in growth and significant in ecological restoration effect.

A transgenic method for perennial ryegrass includes such steps as providing the induced calli of mature embryo, infecting by the Agrobacterium tumefaciens with antibiotic resistant gene, and antibiotic screening to obtain resistance plant.

The invention belongs to the field of engineering technique for ecological greening town in subtropical zones. The employed combined lawn strains are Ling zhi tall fescue, hound tall fescue, beck tall fescue, Jiao zhan tall fescue, midnight English grass, De bi perennial ryegrass and common Bermuda grass, sowing seeds according to the proportion of tall fescue strains 50%, English grass strains 20%, perennial ryegrass strains 10% and Bermuda grass 20%, for two working times and even sowing, through scientific caring and management, in areas of normal climate and management such as Nan jing, the lawn yearly green period is above 320 days, the oversummering and overwintering rate is above 95%, the structure is stable, there are little weeds, the lawn is temperature-resistant, cold-resistant and trample-resistant, and the cost of lawn forming and caring is low, suitable for forming lawns in the green place, square and sportsground.

This invention involves a method of N.coenophialum and N.lolii which uses real-time fluorescence PCR technology to detect tall fescue and perennial ryegrass endophytic fungi, namely. It belongs to grass fungus detection area of the original illness. This invention relates to two Taqman probes and primers: a set is universal primers and probe for the grass fungus Neotyphodium in monochrome fluorescence PCR detection. The other set is specific primers and probes for forage and N.loli N.coenophialum of the two-color fluorescent PCR detection. The invention establishes a simple, rapid, specific, sensitive real-time fluorescence PCR detection method, and its sensitivity reaches a grass seeds. Detection time of the conventional method of can be at least cur from one month to 7-8 hours.

The present invention provides a method of establishing efficient perennial ryegrass gene gun conversion system and its application. By means of regulating the effect factors of gene gun bombardment process, including the size of the bombarded callus block, gold powder coated matter, gold powder diameter, bombardment height, bombardment times, etc. as well as screening pressure and time of the screening agent, the present invention establishes the perennial ryegrass genetic transformation system successively. The genetic transformation system is applied in establishing transgenic perennial ryegrass and obtaining drought resistant, salt resistant and low temperature resistant transgenic perennial ryegrass plant.

The present invention includes the following steps: obtaining embryonic callus acceptor material via high frequency regeneration system, culturing donor strain, determining concentration of ablastin, optimizing agrobacterium transforming condition, screening and culturing transformed callus and molecular detection of plant regeneration and resisting plant. By means of optimizing the conditions for transforming agrobacterium, including bacterium liquid concentration, infection time, infection condition, co-culture time, ablastin concentration, etc., the present invention provides agrobacterium mediated transformation system suitable for perennial ryegrass. The present invention solves the technological problem of applying agrobacterium mediating technology in genetic transformation of gramineous plant and establishes efficient agrobacterium mediated transformation system with embryonic callus as acceptor material for perennial ryegrass.

American ryegrass varieties and seed used to produce the grass are provided. The grass is useful as a short lived, improved turfgrass on golf courses, athletic fields and other areas using seeded turfgrasses. The grass is useful in overseeding of dormant warm season grasses since its lack of heat tolerance allows for a smooth transition for the warm-season grass. The grass will provide a green cover during the winter months but will die out when the temperatures increases to the point which allow the warm-season grass to grow. In more temperate climates American ryegrass will provide a permanent turf more cold tolerance, with good winter color, and better drought tolerance and persistence than perennial ryegrass.

The invention discloses a method for establishing perennial ryegrass genetic conversion receptor system through embryoid generation approach, which mainly consists of using perennial ryegrass' mature seeds as material, obtaining callus through medium induced culture, carrying out subculture, inducing embryoid and regenerated strains in callus induced medium and regenerated medium, charging 2,4-D,6-BA,NAA and hydrolyse casein CH of a predetermined proportion into the induced callus medium, so as to effectively increase the inductivity of the callus.

The invention discloses a method for passivating and slowly killing ryegrass in an overseeding lawn. The method comprises the following steps of (1) carrying out low shearing on perennial ryegrass before premedication, controlling the height to be 2.5-3cm and following the 1 / 3 principle of lawn trimming; (2) firstly spraying a benzyl dichloride inhibitor on soil of the perennial ryegrass when warm season turfgrass turns green, and passivating the perennial ryegrass; and (3) killing the perennial ryegrass by using trifloxysulfuron sodium 15-20d after using the inhibitor. Compared with the prior art, after the method for passivating and slowly killing the ryegrass in the overseeding lawn is used, the height of the perennial ryegrass is significantly reduced, the leaves are obviously upward, the underlying light transmittance of the ryegrass is significantly increased and the warm season turfgrass can turn green about 34 days earlier.

The invention provides a grass / white clover land degeneration degree grading method and application thereof and relates to the technical field of grassland agriculture. According to the grading method, grassland is divided into non-degraded grassland, low-grade degraded grassland, medium-grade degraded grassland and high-grade degraded grassland according to standards; particularly, grass / white clover land degeneration degree is graded by measuring annual dry matter yield of forage grass, dry matter components of sowed perennial ryegrass and orchardgrass, dry matter component of white clover,tillering density of sowed perennial ryegrass, tillering density of orchardgrass, stolon density of white clover, and pH of soil of grassland at the depth of 0-10cm. The grading method can complete grading of degeneration degree of grass / white clover land simply and practically.

The invention provides an interplanting method for zoysia plants. The method comprises the steps of: (1) setting of interplanting time; (2) low cutting of zoysia; (3) applying of a growth inhibitor; (4) nutrient soil or river sand covering; (5) cold-season grass seed sowing; (6) post-sowing treatment; (7) post-sowing management; (8) trimming before green-turning of zoysia; (9) fertilization: composite fertilizer is applied after cutting and then normal management is performed. The interplanting method for zoysia plants has the advantages that the emergence rate of cold-season grass such as perennial ryegrass to reach 95% and above and cold-season grass can keep green in a withered and yellow period of zoysia, so that the problem of difficult interplanting of zoysia is solved; the method can promote wide application of zoysia plants.

A method for planting pasture on a sunny side with the gradient larger than 20 degrees in abandoned woodland of a cold highland area includes the steps of planting the pasture on the slope sunny sidewith the gradient larger than 20 degrees in the abandoned woodland in the cold highland area at the altitude of 2000-2500 m in the southwest of the Yunan Province in China, wherein 9-11% of annual ryegrass seeds, 28-32% of perennial ryegrass seeds, 28-32% of orchardgrass seeds and 28-32% of trifolium repens seeds are sown in a hybrid mode during pasture plantation; conducting herding when the pasture grows to have a grass layer height larger than 5 cm; cutting the pasture to be aired into green dry grass or ensilage when the pasture is surplus, wherein the crop reserving height is 5 cm duringpasture cutting. The cold highland area pasture planting method is suitable for being implemented in the southwest of the Yunan Province in China, and the planted pasture is rapid in reproduction, good in growth and remarkable in ecological repairing effect.

The invention is concerned with the application method that uses the straw and the lawn plant to recover the housing refuse with heavy metal, it is: the 10 to 80 of housing refuse compost mix with 1 to 10 of straw to make the lawn plant culture substrate, seeds on the lawn plant culture substrate and give water every day, uses the ICP-AES method to test the heavy metal content after distilling bythe lawn plant from cradle the upside of the lawn plant; the result shows with the paddy straw, the recover actuation of the Cr, Ni and Cd by the secular-grow ryegrass, and the recover actuation of the Cr and Ni by festuca arundinacea are big foreground.

Perennial ryegrass plants with a spreading habit are provided. The perennial ryegrass plants of the present invention display a growth habit with intertwining tillers that form a very dense sward of 10,000 tillers or more per square foot within one growing season. The ryegrass plants of the present invention also display secondary tillers, something not seen in currently available commercial perennial ryegrass varieties. The spreading growth habit of the plants of the present invention makes a very attractive turfgrass when grown for turf and also provides high seed production.

Perennial ryegrass plants with a spreading habit are provided. The perennial ryegrass plants of the present invention display a growth habit with intertwining tillers that form a very dense sward of 10,000 tillers or more per square foot within one growing season. The ryegrass plants of the present invention also display secondary tillers, something not seen in currently available commercial perennial ryegrass varieties. The spreading growth habit of the plants of the present invention makes a very attractive turfgrass when grown for turf and also provides high seed production.

The invention discloses a method for regulating airport insect community diversity by adopting lolium perenne planting. The method comprises the steps that firstly, lolium perenne and festuca elata are selected as test plants for single lawn planting, four test areas are randomly selected at an airport, the size of each area is 15m*20m, the middle interval is 2m, ripping is carried out with a rooter, grass seeds are not sown to make vegetation grow naturally, and clipping is not carried out; a main clipping area is grinded twice each year, clipping treatment once is carried out on the vegetation each half month; ripping is carried out in the lolium perenne lawn test area, the lolium perenne seeds are sown, the total seeding quantity is 10 kilograms, 50 ditches are dug up, an interval of each ditch is 0.3 meter, and seeding quantity of each ditch is 0.2 kilogram; the quantity of insects is randomly recorded in each sample area, later, the collected insects are taken back to a laboratoryfor sorting and identification. According to the method for regulating the airport insect community diversity by adopting the lolium perenne planting, lawn plant lolium perenne not growing in an original habitat is planted at the airport. The invention aims at exploring the effect of single lawn plants on the insect community and diversity of the airport.

The invention discloses a pasture planting method of a mountainous region with a slope of 10-25 DEG of a cold highland area. The method is that pasture planting is carried out in the mountainous region with the slope of 10-25 DEG of the cold highland area with an altitude of 2000-2500 m in the southwest of Yunnan Province, China; the pasture planting is that 9-11% of annual ryegrass Abundant seeds, 28-32% of perennial ryegrass Mathilde seeds, 28-32% of dactylis glomerata Donata seeds and 28-32% of white clover Riesling seeds are mixed sowed in a sunny face of the mountainous region; and 9-11%of annual ryegrass Abundant seeds, 28-32% of perennial ryegrass Mathilde seeds, 28-32% of dactylis glomerata Amba seeds and 28-32% of white clover supper Haifa seeds are mixed sowed in a shady face ofthe mountainous region. The invention provides the cold highland area pasture planting method suitable for implementation in the southwest of Yunnan Province, with rapid pasture propagation, good pasture growth, and significant ecological restoration effect.