Method for simultaneously preparing trehalose and glucolactone

A technology of gluconolactone and gluconic acid, which is applied in the field of bioengineering, can solve problems such as limitation, lack, and lack of high conversion rate, and achieve the effect of reducing conversion time and increasing conversion rate

Active Publication Date: 2016-12-21
德州汇洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many production methods for gluconic acid and lactone, there are certain restrictions
[0011] In summary, there is currently a lack of methods for producing trehalose with a high conversion rate in the art, so it is urgent to develop an efficient and stable preparation method for trehalose, and the prior art also lacks the ability to produce trehalose while obtaining Production method of gluconolactone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: Simultaneous production of trehalose and gluconolactone (mass fraction is 35% starch)

[0074] In the first step, amylase converts 35% of the starch into maltodextrin:

[0075] Add 30L of starch milk with a mass fraction of 35% in a 50L tank, control the pH to 5.8, and control the temperature at 100°C after adding high-temperature amylase. The activity unit of the high-temperature amylase is 5000U / mL, and the dosage is 6.0g of high-temperature amylase / 100g starch, treated for 3 hours, liquefied starch into maltodextrin with DE value of 9.5;

[0076] The second step is to remove branches and add mixed enzymes to obtain trehalose:

[0077] Control the temperature at 60°C, pH 5.0-5.5, add pullulanase, the activity unit of the pullulanase is 1000U / mL, the dosage is 1.0g pullulanase / 100g maltodextrin, after 6 hours of treatment, put Remove branched chains of maltodextrin, control the temperature at 40°C, pH 5.5-6.0, add maltooligosaccharide-based trehalose s...

Embodiment 2

[0084] Embodiment 2: Simultaneous production of trehalose and gluconolactone (mass fraction is 40% starch)

[0085] In the first step, amylase converts 40% of the starch into maltodextrin:

[0086] Add 30L of starch milk with a mass fraction of 40% in a 50L tank, control the pH to 6.0, and control the temperature at 105°C after adding high-temperature amylase. The activity unit of the high-temperature amylase is 8000U / mL, and the dosage is 3.5g of high-temperature amylase / 100g starch, treated for 4 hours, the starch was liquefied into maltodextrin with a DE value of 12.4;

[0087] The second step is to remove branches and add mixed enzymes to obtain trehalose:

[0088] Control the temperature at 60°C, pH 5.0-5.5, add pullulanase, the activity unit of the pullulanase is 1500U / mL, the dosage is 0.5g pullulanase / 100g maltodextrin, after 6 hours of treatment, put Remove branched chains of maltodextrin, control the temperature at 40°C, pH 5.5-6.0, add the crude enzyme solution o...

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Abstract

The invention provides a method for simultaneously preparing trehalose and glucolactone. The method comprises the steps that amylase is used for converting starch into maltodextrin; a branch of the maltodextrin is removed, and maltooligosyl trehalose synthase and malt oligosaccharyl trehalose hydrolase are used for obtaining trehalose; glucose oxidase and catalase are fed, and generated glucose is converted into sodium gluconate; saccharifying enzymes are added, after saccharifying is finished, glucose oxidase and catalase continue to be fed for reacting, and when the glucose content is lower than 1 g/L, reacting is finished; enzyme deactivation, decoloration, filtering and impurity removal are carried out. According to the method for simultaneously preparing trehalose and glucolactone, the conversion time of trehalose can be obviously shortened, the conversion rate is obviously increased to be larger than 78%, and meanwhile glucolactone can be produced.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for simultaneously preparing trehalose and gluconolactone. Background technique [0002] Trehalose is a non-reducing sugar composed of two glucose molecules bonded by α,α-1,1 glycosidic bonds. It widely exists in bacteria, yeast, algae, fungi, insects and some stress-resistant plants. Trehalose has a non-specific protective effect on biological macromolecules such as cell membranes, proteins, and nucleic acids, and has good water absorption, stability, and is not easy to caramelize. Exogenous trehalose also has a non-specific protective effect on organisms and biological macromolecules. The unique biological properties of trehalose make it have important applications in food, medicine, cosmetics, vaccines and other fields. [0003] The production methods of trehalose mainly include microbial extraction, fermentation and enzymatic conversion. [0004] Microbial extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P17/06
CPCC12P17/06C12P19/12C12P19/14
Inventor 赵伟段莹莹任峰曹大鹏
Owner 德州汇洋生物科技有限公司
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