Method for simultaneously preparing trehalose and glucolactone
A technology of gluconolactone and gluconic acid, which is applied in the field of bioengineering, can solve problems such as limitation, lack, and lack of high conversion rate, and achieve the effect of reducing conversion time and increasing conversion rate
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Embodiment 1
[0073] Embodiment 1: Simultaneous production of trehalose and gluconolactone (mass fraction is 35% starch)
[0074] In the first step, amylase converts 35% of the starch into maltodextrin:
[0075] Add 30L of starch milk with a mass fraction of 35% in a 50L tank, control the pH to 5.8, and control the temperature at 100°C after adding high-temperature amylase. The activity unit of the high-temperature amylase is 5000U / mL, and the dosage is 6.0g of high-temperature amylase / 100g starch, treated for 3 hours, liquefied starch into maltodextrin with DE value of 9.5;
[0076] The second step is to remove branches and add mixed enzymes to obtain trehalose:
[0077] Control the temperature at 60°C, pH 5.0-5.5, add pullulanase, the activity unit of the pullulanase is 1000U / mL, the dosage is 1.0g pullulanase / 100g maltodextrin, after 6 hours of treatment, put Remove branched chains of maltodextrin, control the temperature at 40°C, pH 5.5-6.0, add maltooligosaccharide-based trehalose s...
Embodiment 2
[0084] Embodiment 2: Simultaneous production of trehalose and gluconolactone (mass fraction is 40% starch)
[0085] In the first step, amylase converts 40% of the starch into maltodextrin:
[0086] Add 30L of starch milk with a mass fraction of 40% in a 50L tank, control the pH to 6.0, and control the temperature at 105°C after adding high-temperature amylase. The activity unit of the high-temperature amylase is 8000U / mL, and the dosage is 3.5g of high-temperature amylase / 100g starch, treated for 4 hours, the starch was liquefied into maltodextrin with a DE value of 12.4;
[0087] The second step is to remove branches and add mixed enzymes to obtain trehalose:
[0088] Control the temperature at 60°C, pH 5.0-5.5, add pullulanase, the activity unit of the pullulanase is 1500U / mL, the dosage is 0.5g pullulanase / 100g maltodextrin, after 6 hours of treatment, put Remove branched chains of maltodextrin, control the temperature at 40°C, pH 5.5-6.0, add the crude enzyme solution o...
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