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Mahonia bealei extract with functions of resisting bacteria, diminishing inflammation and promoting tissue regeneration as well as preparation method and application thereof

A technology of broad-leaved top ten merits and extracts, which is applied in the direction of medical preparations containing active ingredients, anti-inflammatory agents, anti-bacterial drugs, etc., to achieve the effect of increasing economic benefits, wide distribution, and reasonable operation

Inactive Publication Date: 2017-01-04
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the active medicinal ingredients of Tuhuanglian and its medicinal effects.

Method used

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  • Mahonia bealei extract with functions of resisting bacteria, diminishing inflammation and promoting tissue regeneration as well as preparation method and application thereof
  • Mahonia bealei extract with functions of resisting bacteria, diminishing inflammation and promoting tissue regeneration as well as preparation method and application thereof
  • Mahonia bealei extract with functions of resisting bacteria, diminishing inflammation and promoting tissue regeneration as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1. The preparation of broad-leaved top ten meritorious service

[0028] 100g of stems and leaves of broad-leaved top ten meritorious service, decocted and extracted twice with ten times of water (W / V), 1 hour each time, combined the two extracts, concentrated under reduced pressure, and freeze-dried to obtain 12.3g of crude extract. 100g of broad-leaved top ten meritorious service stems and leaves, reflux extraction with 10 times the amount of 60% (W / V) ethanol for 2 times, each time for 1 hour, combined the two extracts, concentrated under reduced pressure, and obtained 13.7g of crude extract after freeze-drying .

[0029] Use HPLC technology to analyze the components of the broad-leaved ten major meritorious service extracts. The separation column is Ultimate AQ-C18 (250mm×4.6mm, 5μm), the separation liquid is composed of acetonitrile (A) and water (B), and the separation scheme is 5%. -8%A at 0-5min, 8%-14%A at 5-30min, 14%-50%A at 30-55min, 50%-90%A at 5...

Embodiment 2

[0030] Example 2 Cytotoxicity test of the extract of the ten major contributions of broad leaves

[0031] MTT assay is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at 540-720nm with an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. Within a certain cell number range, the amount of MTT crystal formation is proportional to the cell number. This method has been widely used in the activity detection of some biologically active factors, large-scale antitumor drug screening, cytotoxicity experiments, and tumor radiosensitivity determination. It is characterized by high sensitivity and economy. Macrophag...

Embodiment 3

[0035] Example 3 In vitro anti-inflammatory activity experiment of broad-leaved ten major meritorious service extracts

[0036] COX-2, iNOS, IL-1β, and IL-6 are extremely important inflammatory factor products, which play an important role in regulating inflammatory wound pain, redness, etc., and the higher their expression levels, the more severe the inflammatory response. On the contrary, its expression level decreases, and the degree of inflammatory response also decreases accordingly, so its expression level is an important index to measure the degree of inflammatory response. The mouse macrophage Raw264.7 induced by lipoprotein (LPS) was used to establish an in vitro anti-inflammatory model, and Real-time PCR was used to detect the changes of the four indicators after adding the extract of the broad-leaved ten-legged fruit.

[0037] The experimental results showed that the expression levels of the four factors decreased in a dose-dependent manner after adding the water an...

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Abstract

The invention discloses application of a mahonia bealei extract serving a unique active ingredient in preparation of a medicament capable of treating chronic inflammation wounds, wherein the chronic inflammation wounds include wounds caused by any one of chronic mastitis, diabetic feet, burnt wounds, chronic ulcer and Crohn disease. The mahonia bealei extract can be prepared into a tablet, a capsule, an injection, an externally-applied solution, lotion, a liniment, an enema, an ointment, a paste, a patch, a slow release agent or a controlled release agent by use of a medically acceptable carrier or excipient. A preparation method of the mahonia bealei extract comprises the following steps of: extracting plant mahonia bealei stems and leaves, which serve as raw materials, by use of water, ethanol or aqueous alcohols, performing filtration, performing concentration until the volume is small, and performing freeze drying to obtain a product for later use. Compared with the prior art, the mahonia bealei extract has the beneficial effects that (1) the medicament preparation process is simple and the operation is reasonable; (2) an in-vivo external anti-inflammation pharmacological experiment proves the application value of the mahonia bealei extract in treating chronic inflammation wounds and provides a theoretical foundation and an experimental foundation for clinical development of the mahonia bealei extract; and (3) research on economic value of mahonia bealei has been blank, medicinal research and drug effect research are performed, effective protection is realized on the basis of utilization, and effects of ex-situ conservation and sustainable development can be achieved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method for extracting effective fractions from natural plant broad-leaved top ten fruits (Natum chinensis) and its application in chronic inflammatory wounds. Background technique [0002] Globally, there are more than 2 million patients with chronic inflammatory wounds, and the number is increasing at a rate of 10-15% every year. Chronic refractory wounds are common and frequently-occurring diseases that seriously endanger people's health, such as diabetic foot ulcers, Crohn's disease, irritable bowel syndrome, and lobular granulomatous mastitis. Due to the complex underlying disease, the infiltration of a large number of inflammatory cells and factors in the affected area, severe tissue damage and necrosis, and the limited efficacy of antibiotic or hormone therapy, how to accelerate the healing of chronic inflammatory wounds has become an urg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/29A61P29/00A61P17/02A61P15/14A61P3/10A61P1/00A61P31/04
CPCA61K36/29A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61P1/00A61P3/10A61P15/14A61P17/02A61P29/00A61P31/04
Inventor 林毅王志宇司徒红林王能
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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