Nocardia farcinica bacteria strain and application thereof
A technology of Nocardia spp. and Nocardia spp., which is applied to Nocardia spp. strains and its application fields, can solve the problem of low control efficiency, slow time effect, root-knot nematode poisoning effect and related mechanisms have not been reported yet. and other problems, to achieve the effect of fast poison killing timeliness and high prevention effect
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Embodiment 1
[0030] Use a pipette gun to take 100 μl of Nocardia aeruginosa cultured at 37°C for 48 hours and add it dropwise to the center of the sterile water agar plate, then add another 300 μl of BHI, and spread it evenly on a 60 mm sterile water agar plate with a spreader On the water agar plate, add 300 μl of BHI culture solution or sterile water as a control. Place the water agar plate in an ultra-clean bench and blow dry, then add 50 μl of J2 suspension to the center of the water agar plate. Place the water agar plate added with J2 at 28°C, and count the survival of J2 under the microscope every 24 hours. (See Table 1)
Embodiment 2
[0032] Use a pipette gun to take 200 μl of Nocardia aeruginosa cultured at 37°C for 48 hours and add it dropwise to the center of the sterile water agar plate, then add another 200 μl of BHI, and spread it evenly on a 60 mm sterile water agar plate with a spreader On the water agar plate, add 400 μl of BHI culture solution or sterile water as a control. Place the water agar plate in an ultra-clean bench and blow dry, then add 50 μl of J2 suspension to the center of the water agar plate. Place the water agar plate added with J2 at 28°C, and count the survival of J2 under the microscope every 24 hours (see Table 1).
Embodiment 3
[0034]Use a pipette gun to take 300 μl of Nocardia aeruginosa cultured at 37°C for 48 hours and add it dropwise to the center of the sterile water agar plate, then add another 100 μl of BHI, and spread it evenly on a 60 mm sterile water agar plate with a spreader On the water agar plate, add 400 μl of BHI culture solution or sterile water as a control. Place the water agar plate in an ultra-clean bench and blow dry, then add 50 μl of J2 suspension to the center of the water agar plate. Place the water agar plate added with J2 at 28°C, and count the survival of J2 under the microscope every 24 hours (see Table 1).
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