Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting molecular weight and molecular weight distribution coefficient of medical sodium hyaluronate gel

A technology of sodium hyaluronate and molecular weight distribution, applied in the field of chemical inspection, can solve problems such as low universality, and achieve the effects of simple operation, good stability and cost saving.

Inactive Publication Date: 2017-01-04
ZHEJIANG JINGJIA MEDICAL TECH CO LTD
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a medical sodium hyaluronate gel for the determination of the weight average molecular weight and molecular weight distribution coefficient of sodium hyaluronate gel in the medical sodium hyaluronate gel in the existing industry standards. Determination method of weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in glue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting molecular weight and molecular weight distribution coefficient of medical sodium hyaluronate gel
  • Method for detecting molecular weight and molecular weight distribution coefficient of medical sodium hyaluronate gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The invention provides a method for detecting the weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in medical sodium hyaluronate gel:

[0024] According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0514 molecular exclusion spectroscopy.

[0025] Experimental method and process:

[0026] 1. Instrument

[0027] Chromatographic instrument: Agilent 1260Infinity high performance liquid chromatography system (Agilent, USA): G1310B unit pump; G1322A online degasser; G1316A column thermostat; G1329B automatic sampler; G1362A differential detector; G7850AA GPC / SEC workstation.

[0028] Analytical balance: BSA224S type (manufacturer: Sartorius)

[0029] Test product: medical sodium hyaluronate gel.

[0030] 2. Chromatographic conditions

[0031] Chromatographic column: gel column Shodex SB-806HQ (8.0mm×300mm);

[0032] Mobile phase: Weigh 11.69g of sodium chloride and 0.2g of sodium azide, add 1000ml of pur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting the weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in medical sodium hyaluronate gel. The method comprises: precisely measuring a proper amount of a medicinal preparation solution, adding a mobile phase to dilute the solution to a to-be-tested sample liquid with the required concentration, performing uniform shaking, performing sample injection, and recording a chromatogram; taking a sodium polystyrenesulfonate reference substance, performing measuring according to the method above, drawing a calibration curve with a GPC software, and using a universal calibration method to obtain weight-average molecular weight and molecular weight distribution coefficient. A laser scattering and gel permeation chromatography combination method is used for detecting the weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in medical sodium hyaluronate gel in the professional standard. The combination method is hard to popularize, for the multi-angle laser light scattering spectrometer is expensive. According to the method, the used device is good in universality, the measurement cost is low, and the result is accurate, reliable and stable.

Description

[0001] Technical field: [0002] The invention relates to the field of chemical testing and the technical field of drug analysis, in particular to a method for detecting the weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in medical sodium hyaluronate gel. [0003] Background technique: [0004] In the existing industry standard, the weight-average molecular weight and molecular weight distribution coefficient of sodium hyaluronate in medical sodium hyaluronate gel are determined by laser scattering-gel permeation chromatography, but the multi-angle laser light scattering instrument is expensive and difficult to popularize. . Therefore be badly in need of inventing a kind of utilizing the method detection of the present invention, the instrument universality that uses is strong, and measurement cost is low, the result is accurate, reliable, good stability in medical sodium hyaluronate gel weight-average molecular weight and mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王旭敏熊祥龙
Owner ZHEJIANG JINGJIA MEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com