Detection method of aflatoxin B1
A technology of aflatoxin and detection method, applied in the field of detection of aflatoxin B1, can solve the problems of recovery rate fluctuation, long operation time, interference, etc., and achieve rapid detection, simple steps and good stability
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Embodiment 1
[0048] A detection method for aflatoxin B1, comprising the following steps:
[0049] 1. Pre-processing.
[0050] The sample to be tested is extracted with the extraction solution, and in this embodiment, the sample to be tested is soy sauce.
[0051] The specific pretreatment method is as follows: Weigh 5.0g of representative samples into a 50mL polystyrene centrifuge tube, add different extraction solutions in different proportions and mix them; vibrate vigorously on an oscillator for 10 minutes at a speed of 150r / min (or vortex for more than 5 minutes); take the liquid and centrifuge it at 4000r / min for 5 minutes (or let it stand for 3 minutes, and then filter it with quantitative filter paper); take 0.5mL of supernatant or filtrate, and then add diluent in different proportions to obtain the solution to be tested. Shake the solution to be tested for 5 s or shake it by hand, and take 50 μL for analysis.
[0052] Second, detection.
[0053] Take the solution to be tested, ...
Embodiment 2
[0120] A detection method for aflatoxin B1, comprising the following steps:
[0121] 1. Pre-processing.
[0122] The sample to be tested is extracted with the extraction solution, and in this embodiment, the sample to be tested is bean paste.
[0123] The specific pretreatment method is as follows: take 4.0g of representative samples and place them in a 50mL polystyrene centrifuge tube, add 10mL of methanol as the extraction solution and mix them; vibrate vigorously on a shaker for 10 minutes at a speed of 150r / min ( or vortex for more than 5min); take the liquid and centrifuge it at 4000r / min for 5min (or let it stand for 3min, and then filter it with quantitative filter paper); take 0.1mL of the supernatant or filtrate, and then add 1.9mL deionized water as a diluent to obtain the solution, the dilution factor is 50. Shake the solution to be tested for 5 s or shake it by hand, and take 50 μL for analysis.
[0124] Second, detection.
[0125] Detect according to the metho...
Embodiment 3
[0134] A detection method for aflatoxin B1, comprising the following steps:
[0135] 1. Pre-processing.
[0136] The sample to be tested is extracted with the extraction solution. In this embodiment, the sample to be tested is cooking wine, rice wine, pipa wine, and white vinegar.
[0137] Concrete pretreatment method is: take by weighing 5.0g representative sample and insert in 100mL tool stopper Erlenmeyer flask, add the methyl alcohol of 25mL60% and mix it as extraction solution; Vigorously vibrate 10 minutes on the shaker, the rotating speed is 150r / min ( Or vortex for more than 5min); take the liquid and centrifuge at 4000r / min for 5min (or let it stand for 3min, and then filter it with quantitative filter paper); take 1mL of supernatant or filtrate, and then add 5mL deionized water as a diluent to obtain the solution to be tested. The pH value of the solution to be tested should be between 6 and 8, which can be adjusted with "NaOH" and "HCL". Shake the solution to be t...
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