Efficient breeding method adopting multi-female-parent one-male-parent mode to prepare oilseed rape hybrid
A hybrid and rapeseed technology, applied in the field of crop genetics and breeding, can solve the problems affecting the purity of seed production, high cost of seed production, low yield, etc., achieve important scientific value and production application value, large amount of seed production, and accelerate the breeding process Effect
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Embodiment 1
[0051] Example 1: Taking 2013 as an example, using 18 inducible sterile lines or nuclear male sterile lines, the specific implementation steps of preparing hybrids by the method of multiple mothers and one father (see Table 4):
[0052] Table 4: Hybrids configured with 18 inducible sterile lines using a common male parent pollination line, male parent ZY1
[0053]
[0054] 1. Parental selection: The selected male parent pollination line ZY1 has the following characteristics: strong growth potential, dispersed plant type, high oil content, medium to high stalk, good horniness, mid-to-late maturity, good resistance, and midwinter. The selected 18 inducible sterile lines have 3-4 characteristics such as short stem early maturity, compact plant type, concentrated flowering period, good yield, high oil content, good general combining ability, lodging resistance and disease resistance, and suitable for machine harvesting. , All of them are semi-winter high-generation strains.
[0055] 2. ...
Embodiment 2
[0066] Example 2: Taking 2015 as an example, the implementation steps of using 15 cytoplasmic male sterile lines to configure hybrids using the method of multiple mothers and one father:
[0067] 1. Parental selection: The selected male parent pollination line SR3 is an excellent restorer line for cytoplasmic male sterility, dual-use in winter and spring. Its characteristics are as follows: early maturity and short stalks, short branches, straight siliques, good yellowing, compact plant type, strong resistance and tolerance, and high oil content. The 15 cytoplasmic male sterile lines are all Shaan 2A cytoplasm, which belong to spring sterile lines. Its characteristics are: strong growth potential, good yield, high plant height, mid-to-late maturity, good openness, easy seed production, poor lodging resistance, scattered plant types, and easy to be affected by low temperature in the early stage to produce micronized self-fertilization.
[0068] 2. Safety isolation: the same as the ...
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