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Fermentation medium for improving nikkomycin yield and method using the same

A fermentation medium and nikkomycin technology, applied in the field of microorganisms, can solve the problems of large consumption of raw materials, low yield and the like, and achieve the effects of increasing yield, low cost and simple scheme

Active Publication Date: 2017-01-18
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for preparing nikkomycin in the prior art has the problems of low yield and large consumption of raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment specifically provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes BJX005, comprising the following steps:

[0028] Step (1): inoculate the seed liquid of Streptomyces circulogenes in a fermentation medium with an inoculum size of 4% to obtain a fermentation broth; the medium comprises the following components: by mass percentage , corn starch 3%, soybean meal powder 3%, yeast powder 0.2%, sodium chloride 0.6%, ammonium sulfate 0.04%, calcium carbonate 0.5%, polysiloxane defoamer DF-805 0.02%, threonine 0.0003% , the balance is water; the pH is adjusted to 6.8; the fermentation culture is a vibration culture, and the rotation speed of the vibration culture is 220r / min, the fermentation time is 70h, and the fermentation temperature is 28°C;

[0029] Step (2): extracting nikkomycin from the fermentation broth.

[0030] The nikkomycin prepared in this example was tested and found to have a content of 42800 μg / mL.

Embodiment 2

[0032] This example specifically provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes BJX005. Contains the following steps:

[0033] Step (1): Inoculate the seed liquid of Streptomyces circulogenes in a fermentation medium with an inoculum size of 10% to obtain a fermentation broth; the fermentation medium comprises the following components: by mass percentage 2.5% corn starch, 2% soybean meal, 0.3% yeast powder, 0.4% sodium chloride, 0.05% ammonium sulfate, 0.45% calcium carbonate, polysiloxane defoamer SXP-101-1 (purchased from Anhui Suzhou China Resources Chemical Co., Ltd.) 0.025%, threonine 0.0001%, and the balance is water; adjust the pH to 7.5; the fermentation culture is vibration culture, and the vibration culture speed is 200r / min, the fermentation time is 80h, and the fermentation temperature is 25°C;

[0034] Step (2): extracting nikkomycin from the fermentation broth.

[0035] The content of nikkomycin prepared in this example was 4...

Embodiment 3

[0037] This example specifically provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes BJX005. Contains the following steps:

[0038] Step (1): Inoculate the seed liquid of Streptomyces circulogenes in a fermentation medium with an inoculum size of 6% to obtain a fermentation broth; the fermentation medium comprises the following components: by mass percentage 2.5% corn starch, 2.5% soybean cake powder, 0.25% yeast powder, 0.5% sodium chloride, 0.05% ammonium sulfate, 0.45% calcium carbonate, GPE type polyether defoamer namely foam enemy (purchased from Jinan Australia Xing Chemical Co., Ltd.) 0.025%, threonine 0.0005%, and the balance is water; adjust the pH to 6.3; the fermentation culture is vibration culture, and the vibration culture speed is 210r / min, the fermentation time is 60h, and the fermentation temperature is 27 ℃;

[0039] Step (2): extracting nikkomycin from the fermentation broth.

[0040] The content of the nikkomycin prepared in...

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PUM

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Abstract

The invention relates to the field of microbes and provides a Streptomyces ansochromogenes fermentation medium and a method for preparing nikkomycin through the medium. Through use of a proper amount of threonine in the fermentation medium, further optimization of types and use amounts of components of the fermentation medium and control of other culture conditions, a nikkomycin yield is improved. The fermentation medium has a nikkomycin yield of 42800 micrograms per milliliter, has a simple formula and a low cost and is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of microorganisms, and specifically provides a fermentation medium for Streptomyces chromogenes and a method for preparing nikkomycin by using the medium. Background technique [0002] Nikkomycin is a nucleoside peptide antibiotic that can effectively inhibit the synthesis of chitin, one of the skeleton components of fungal cell walls, thereby effectively inhibiting the growth of fungi, insects, and worms. The fermented liquid of nikkomycin contains more than 20 kinds of components, among which nikkomycin Z and X are the main bioactive components. Nicomycin is easy to degrade in nature and has no residue; it is relatively non-toxic to mammals, bees, plants, etc. As an inhibitor of fungal cell wall chitin synthase, nikkomycin has broad application prospects in the prevention and treatment of fungal infectious diseases in animals, plants and humans. In agriculture, nikkomycin has a huge market for the prevention and t...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 周贤龙石怀月刘静
Owner 牡丹江佰佳信生物科技有限公司
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