Compound microorganism preparation against sclerotinia rot of colza and preparing method thereof

Abstract

The invention discloses a compound microorganism preparation against sclerotinia rot of colza and preparing method thereof. The compound microorganism preparation comprises fermentation broth of streptomyces resistomycificus, fermentation broth of no-branch streptomyces, fermentation broth of bacillus of tequila, fermentation broth of bacillus sim and fermentation broth of stroma trichoderma; the bacterial species in the compound microorganism preparation against sclerotinia rot of colza and preparing method thereof can comprehensively make use of antibiosis, competition, hyperparasitism and bacteriolysis between microorganisms or bacterial species and induce raps to resist diseases by secondary metabolite, generate a plurality of antibiotics such as resistomycin, acetomycin and macrolide bacteria, and generate ultra-high effects of inhibiting pathogen of sclerotinia rot of colza; the 5 bacterial strains used in the compound microorganism preparation against sclerotinia rot of colza and preparing method thereof are drawn by being separated from soil, thereby, with effects of root trace plant growth promotion, which breed and grow on the surface of crops, in the plants or soil while secreting some secondary metabolite to plants in a targeted way, enhancing plants' nutrient absorption and stimulating plants to grow in effects of fertilizer.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and application of microbial preparations, and in particular relates to a compound microbial preparation for preventing and treating rape sclerotinia and a preparation method thereof. Background technique [0002] Rapeseed is an important oil crop in my country. Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum is an important disease worldwide. It occurs widely in my country and is seriously harmful. The incidence rate is 10%-30% in general years. The incidence rate of severe plots is more than 80%, resulting in severe production reduction and quality decline. [0003] Because Sclerotinia sclerotiorum has: (1) can live tenaciously in soil, and very easily accumulates in soil; body or infect seedling crops or use nutrients in the soil to grow and form more sclerotias, one sclerotia can produce many ascus discs, and the germination duration of sclerotia in the spri...

AI Technical Summary

Problems solved by technology

Promoting disease-resistant varieties is one of the most economical and effective measures in plant disease control, but rape does not show vertical resistance to sclerotinia, and no effective disease-resistant varieties have come out yet.

Therefore, for a long time, the prevention and treatment of this disease mainly adopts the spraying of chemical agents on the above-ground parts during the flowering period, but the control effect is not stable.

In addition, the long-term large-scale use of pesticides has serious legacy problems: first, drug-resistant strains have appeared in the Sclerotinia population, such as Pan Yilou (1998) reported that anti-polybacterial strains have appeared in the Sclerotinia population Lingao-resistant strains; second, pollute the environment and harm human health

[0008] However, the research results are still in the laboratory stage, and there are few reports about its wide application in the field for large-scale disease control; the research on the use of biocontrol bacteria to control the disease is mainly the study of a single strain. However, due to the diversity and synchronous evolution of pathogenic bacteria, the existing single antagonistic strains cannot achieve ideal control effects due to their single mode of action, unstable control effect, and production and use. Many biological functions need to rely on the synergy between two or more strains of bacteria to play a better role