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Application of Adjudin in preparing medicine for inhibiting glial scar formation

A technique for glial scars and drugs, applied in the field of preparation of drugs for inhibiting the formation of glial scars, to achieve the effects of inhibiting proliferation and migration, promoting recovery, and improving behavior

Inactive Publication Date: 2017-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, no studies have confirmed that Adjudin can be used as a drug for inhibiting glial scars after central nervous system damage

Method used

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  • Application of Adjudin in preparing medicine for inhibiting glial scar formation
  • Application of Adjudin in preparing medicine for inhibiting glial scar formation
  • Application of Adjudin in preparing medicine for inhibiting glial scar formation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Mouse modeling and cell culture:

[0020] The mice used in the study in this example are ICR mice. A middle cerebral artery embolism model was performed. Seven days after modeling, Adjudin was injected intraperitoneally at a dosage of 50 mg / kg. Behavioral tests were performed every seven days, and the mice were sacrificed on the 35th day, and brain slices were taken. The cells used were primary astrocytes. Astrocytes were obtained from the brains of newborn mice, that is, mice within 24 hours of birth, and the medium was changed two days later for adherent culture.

[0021] 2. Glial scar staining experiment

[0022] After perfusion of the cerebellum, frozen sections were blown dry and fixed with pre-cooled anhydrous methanol for 10 minutes, washed 3 times with PBS, 5 minutes each time; blocked with 10% Donkey serum (containing 0.1% Triton-100) at room temperature for 1 hour ; Wash 3 times with PBS, 5 minutes each time, prepare GFAP primary antibody with 1% Donke...

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PUM

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Abstract

The invention belongs to the field of medicine preparation, and particularly relates to application of Adjudin in preparing medicine for inhibiting glial scar formation. The glial scar is formed by activation and proliferation of astrocyte after central lesion. The medicine is at least in a form of tablets, capsules, granules, dripping pills, suspensions, syrup, enteric-coated preparation, emulsion suspension and injections. Experiments show that Adjudin can effectively reduce size of the glial scar of mice after brain tissue injury; in an in-vitro primary cell scratch model, Adjudin remarkably inhibits proliferation and migration of astrocyte cells, improves ethology of the mice after brain injury and promotes recovery, so that Adjudin has important application value in preparing the medicine for inhibiting glial scar formation.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and particularly relates to the application of Adjudin in the preparation of drugs for inhibiting the formation of glial scars. Background technique [0002] Glial scar is formed by the activation and massive proliferation of astrocytes after the central nervous system is damaged. In the acute stage after injury, glial scar can inhibit the further expansion of the injury and inhibit the spread of the inflammatory response in the injured tissue. Adverse. In general, the barrier formed by glial scar hinders neuronal regeneration and thus negatively affects recovery after injury. [0003] Adjudin, also known as AF-2364, is found in the prior art as a male contraceptive, is a derivative of the chemotherapy drug lonidamine, and is a class of small-molecule drugs with an indoleic acid structure. The chemical formula of Adjudin is: [0004] [0005] In the prior art, no studies have confirmed ...

Claims

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Application Information

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IPC IPC(8): A61K31/416A61P25/00
CPCA61K31/416
Inventor 夏伟梁杨霄耿可依邵家骧
Owner SHANGHAI JIAO TONG UNIV
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