Method for screening bacillus megatherium for desulfurizing of petroleum product

Abstract

The invention discloses a method for screening bacillus megatherium for desulfurizing of a petroleum product. The method comprises the following steps of 1, putting oil-containing soil into a triangular flask, uniformly shaking, and stilling until completely settling; 2, inoculating a supernatant into the triangular flask containing basic culture liquid, and performing enrichment culture; 3, inoculating enrichment culture liquid into the triangular flask containing the basic culture liquid, oscillating and culturing; 4, diluting diluting liquid in doubles, coating the culture liquid onto a solid flat plate containing a basic culture medium, putting the flat plate into a culture box, and culturing until a bacteria colony is grown; 5, scoring and purifying the single bacteria colony, and performing low-temperature ammonia and nitrogen degrading test, so as to obtain a desulfurizing strain of the petroleum product. The screened bacillus megatherium for desulfurizing of the petroleum product is used for widely degrading DBT (dibenzothiophene) in thepetroleum product, so that the significant meaning is realized for the decreasing of air pollution and acid rain and the restoration of ecological environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a screening method for Bacillus megaterium. Background technique [0002] While the sulfur content required by environmental regulations is getting lower and lower, the demand for gasoline and diesel continues to increase in most countries of the world. [0003] Sulfur is mainly present in fossil fuels in the form of heterocyclic compounds, including dimethyl sulfoxide, thiophene, dibenzothiophene (DBT) and other thiophenes. After burning fossil fuels, sulfur is mainly converted to SO 2 Form into the atmosphere, not only corrode equipment, but also cause serious air pollution, and even form acid rain. Therefore, countries all over the world have enacted increasingly stringent environmental protection regulations, which put forward more stringent requirements for the production of clean fuels. One of the important ways to solve this problem is to carry out deep desulfuriza...

AI Technical Summary

Problems solved by technology

One of the important ways to solve this problem is to carry out deep desulfurization of oil products. The traditional hydrodesulfurization method (HDS) is expensive, requires high temperature and high pressure conditions, and is difficult to operate. It also removes heterocyclic sulfur from thiophene and its derivatives. higher cost

Therefore, HDS has been difficult to meet the increasingly stringent desulfurization requirements

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