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Preparation method of fish protein peptide, prepared fish protein peptide and application

A fish protein and protease technology, applied in the field of fish protein peptide and application, the preparation of fish protein peptide, can solve the problems of wound pain, progressive necrosis, susceptibility to infection, etc., and achieve the effect of simple process and easy operation.

Active Publication Date: 2017-01-25
GUANGDONG BAIWEI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the treatment of scalds, there are problems such as wound pain, progressive necrosis, susceptibility to infection, and scar healing

Method used

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  • Preparation method of fish protein peptide, prepared fish protein peptide and application
  • Preparation method of fish protein peptide, prepared fish protein peptide and application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1. Preparation of Fish Protein Peptides

[0021] Take 4kg of processed minced tilapia meat, put it into a 30L enzymatic hydrolysis tank, add tap water according to the ratio of material to liquid ratio of 1:2.5 (weight ratio), and stir evenly. Protease (500 IU / g) and papain (425 IU / g), after enzymolysis for 5 hours, the temperature was raised to 95°C to inactivate the enzyme for 10 minutes, after the enzymolysis solution was cooled, it was centrifuged in a three-legged centrifuge (2000 rpm / min) to remove slag, use an inorganic tubular ceramic membrane with a membrane pore size of 200nm to perform circulating microfiltration on the centrifuged enzymolysis solution, when the volume of the residual solution is about 10% of the feed solution, stop the operation to obtain the permeate , the permeated liquid is vacuum concentrated to a solid content of more than 20%, and then spray-dried to prepare a dry powder, which is fish protein peptide powder.

[0022] The crude prote...

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PUM

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Abstract

The invention provides a preparation method of a fish protein peptide powder. The preparation method comprises the following steps: adding protease into fish meat paste so as to carry out enzymolysis, performing enzyme denaturalization after enzymolysis, further performing centrifugation and microfiltration by using a ceramic film so as to obtain a fish protein peptide liquid, and drying the fish protein peptide, thereby obtaining the fish protein peptide powder, wherein the protease is selected from one or more of neutral protease, papain and pancreatin; and the addition amount of the protease is 400-1500 IU / g. The preparation method is simple in process, easy to operate and beneficial to industrial production; and the produced fish protein peptide is administrated in a rubbing manner, and has a function of promoting heal of scalded skin when the dosage is 50-150mg for each wound surface.

Description

technical field [0001] The present invention relates to the technical field of biological product preparation, and more specifically, to a method for preparing fish protein peptides and the obtained fish protein peptides and applications. Background technique [0002] Due to various accidents, burns often occur. Burns not only cause skin damage, but also cause serious systemic reactions, especially large-area burns. The systemic reactions are very severe, and metabolic disorders and dysfunction of various system organs may occur. , causing serious or even huge damage. There are many problems in the treatment of burns, such as wound pain, progressive necrosis, susceptibility to infection, and scar healing. Medicines for treating burns and scalds are also researched on these four issues. There are two categories: traditional Chinese medicine and western medicine. It is anti-inflammatory, anti-infective, granulogenic, promoting wound healing and promoting epithelial growth. ...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P17/02
CPCA61K38/012C12P21/06
Inventor 洪鹏志杨萍周春霞刘唤明
Owner GUANGDONG BAIWEI BIOTECH
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