A composite filler for filling invisible anti-fog masks
A compound and particle compound technology, which is applied in the direction of respiratory filters, other chemical processes, respiratory protection containers, etc., can solve the problems of single variety, inconvenient use, poor ventilation, etc., and achieve small size, high filtration efficiency, light weight effect
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Embodiment 1
[0022] Adsorptive nano-poultry liver particle composite filler for filling invisible anti-fog masks is prepared from the following raw materials: fresh chicken liver, poultry bone, chitosan, sodium alginate, 0.1mol / L NaOH, 0.1mol / L Phosphate buffer (pH7.4), glutaraldehyde, deionized water, absolute ethanol, tert-butanol, glycerol, acetic acid, trypsin (Nanjing Audofoni Biotechnology Co., Ltd., ≧250U / mg), Proper amount of polyphenol oxidase (Qiyi Biotechnology Co., Ltd., ≧500U / mg).
[0023] Its preparation method comprises the following steps:
[0024] (1) Take 50g of fresh chicken liver and cut it into small pieces of 0.2cm with scissors, add 500ml of 5% trypsin solution and stir slowly for 90min at 25°C until the poultry liver tissue is completely dispersed; add to the chicken liver tissue dispersion 25ml of glutaraldehyde, fixed at 4°C for 3 days, filtered, and rinsed 6 times with 0.1mol / L phosphate buffer (pH7.4). The rinsed sample tissue was eluted with 50%, 70%, 80% and...
Embodiment 2
[0034] The adsorptive nano-duck liver particle composite filler used to fill the invisible anti-fog mask is prepared from the following raw materials in parts by weight: 55g of fresh duck liver, 20g of duck bone, 20g of chitosan, 8g of sodium alginate, 0.1mol / L NaOH, 0.1mol / L phosphate buffer (pH7.4), glutaraldehyde, deionized water, absolute ethanol, tert-butanol, glycerol, acetic acid, trypsin (Nanjing Audofoni Biotechnology Co., Ltd. , ≧250U / mg), polyphenol oxidase (Qiyi Biotechnology Co., Ltd., ≧500U / mg) appropriate amount. Its preparation method comprises the following steps:
[0035] (1) Take 55g of fresh duck liver and cut it into small pieces of 0.2cm with scissors, add 600ml of 5% trypsin solution and stir slowly for 90min at 25°C until the poultry liver tissue is completely dispersed; add to the duck liver tissue dispersion 30ml of glutaraldehyde was fixed at 4°C for 3 days, filtered, and rinsed 6 times with 0.1mol / L phosphate buffer (pH7.4). The rinsed sample tis...
Embodiment 3
[0045] The adsorptive nano-poultry liver particle composite filler used to fill the invisible anti-fog mask is prepared from the following raw materials in parts by weight: 55g of fresh foie gras, 20g of goose bone, 17g of chitosan, 10g of sodium alginate, 0.1mol / L NaOH, 0.1mol / L phosphate buffer (pH7.4), glutaraldehyde, deionized water, absolute ethanol, tert-butanol, glycerol, acetic acid, trypsin (Nanjing Audofoni Biotechnology Co., Ltd. , ≧250U / mg), polyphenol oxidase (Qiyi Biotechnology Co., Ltd., ≧500U / mg) appropriate amount. Its preparation method comprises the following steps:
[0046] (1) Take 55g of fresh foie gras and cut it into small pieces of 0.2cm with scissors, add 600ml of 5% trypsin solution and stir slowly for 90min at 25°C until the foie gras tissue is completely dispersed; add to the foie gras tissue dispersion 30ml of glutaraldehyde was fixed at 4°C for 3 days, filtered, and rinsed 6 times with 0.1mol / L phosphate buffer (pH7.4). The rinsed sample tissu...
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