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Endogenous biocontrol strain for inhibiting damping off of acanthopanax senticosus, and application of endogenous biocontrol strain

A technology of Acanthopanax and blight, which is applied in the field of microorganisms, can solve the problems of soil biological structure damage, serious damage, and increased pesticide residues, and achieve the goals of reducing environmental pollution, protecting ecological balance, increasing income, increasing production, and promoting Effect

Inactive Publication Date: 2017-02-01
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a soil-borne disease caused by fungi in the seedling stage of plants. It has a wide range of occurrence, serious damage, and difficult control. Many years of chemical control have caused pathogenic bacteria to develop resistance to various fungicides, and the development of resistance is amazing, resulting in the use of chemical pesticides. The dosage is continuously increased, resulting in the increase of pesticide residues and the destruction of soil biological structure.

Method used

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  • Endogenous biocontrol strain for inhibiting damping off of acanthopanax senticosus, and application of endogenous biocontrol strain
  • Endogenous biocontrol strain for inhibiting damping off of acanthopanax senticosus, and application of endogenous biocontrol strain
  • Endogenous biocontrol strain for inhibiting damping off of acanthopanax senticosus, and application of endogenous biocontrol strain

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specific Embodiment approach 1

[0024] Embodiment 1: The endogenous biocontrol strain Bacillus sp. SC-JW-14 provided in this embodiment to inhibit Acanthopanax blight is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation address is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The preservation date is September 1, 2016, and the preservation number is CGMCCNo.12921.

[0025] The Bacillus sp. SC-JW-14 of this embodiment is obtained from the Acanthopanax experimental field of the Mudanjiang Forest By-products Research Institute, and is obtained by isolating endophytic bacteria from tissue materials of healthy plants. The specific method is as follows:

[0026] 1. Isolation of endophytic bacteria

[0027] After the harvested plants were rinsed with water, appropriate amount of root, stem, and leaf tissues were selected, weighed, cut into 5mm×5mm tissue pieces, soaked in 95% ethanol for 1min, and then soaked in 1% NaClO so...

specific Embodiment approach 2

[0051] Specific embodiment two: in this embodiment, bacillus (Bacillus sp.) SC-JW-14 can be used for the biological control of Acanthopanax blight, and the control effect experiment is as follows:

[0052] Three experimental groups were set up for pot experiments, which were the healthy control group, the susceptible control group and the control group, with 20 Acanthopanax senticosus plants in each group. Use Rhizoctonia solani (Acanthopanax senticosus) to prepare a concentration of 1 × 10 5 per mL of spore suspension. After surface disinfection of Acanthopanax senticosus seedlings, the healthy control group was injected with 20 μL of sterile water by acupuncture method, and the susceptible control group and control group were each injected with 20 μL of Rhizoctonia solani spore suspension and inoculated in flower pots. And the roots of the plants in the control group were irrigated with 3mL Acanthopanax biocontrol bacteria culture solution (concentration was 0.6×10 7 ~1×10...

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Abstract

The invention discloses endogenous biocontrol strain for inhibiting damping off of acanthopanax senticosus, and application of the endogenous biocontrol strain. The endogenous biocontrol strain is bacillus sp. SC-JW-14, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is No. 3, courtyard 1, Beichen west road, Chaoyang district, Beijing, and the preservation number is CGMCC No. 12921. The bacillus sp. SC-JW-14 can be used for biological control of the damping off of the acanthopanax senticosus, has an antibacterial rate of 69.6% for rhizoctonia solani, and has a control effect reaching up to 59.4%. The pathogenic bacteria of the damping off is taken as a control object, and the antagonistic endogenous strain existing in a healthy plant of the acanthopanax senticosus is separated and screened, so that a foundation is laid for the biological control of the damping off of the acanthopanax senticosus; furthermore, after the endogenous biocontrol strain is reasonably applied, the environmental pollution caused by chemicals can be reduced, the ecological balance is better protected, and the artificial cultivation, income increment and yield increment of the acanthopanax senticosus are greatly improved and promoted; therefore, the endogenous biocontrol strain has certain research significance.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a bacterial strain for inhibiting Acanthopanax blight and application thereof. Background technique [0002] Acanthopanax.sentieosus Harm., a plant of the Araliaceae family, is mainly produced in Northeast my country. It is the most important medicinal material in the genus Aralis. Good health care effect. At present, Acanthopanax not only sells well in China, but also exported to Japan, South Korea, Europe, North America and other countries. It is one of the main Chinese herbal medicines that my country exports to earn foreign exchange, with an annual demand of about 5,000 tons. [0003] Due to the increase in market demand and rising prices, drug farmers have destructively excavated a large number of Acanthopanax rhizomes, and the destructive excavation has prevented them from renewing and forming communities for a long period of time, resulting in a large-scale and rapid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/04A01P3/00C12R1/07
CPCA01N63/30C12N1/205C12R2001/07
Inventor 姜威张淑梅李晶孟利强胡基华刘宇帅陈靖宇
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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