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Detection kit with stable unsaturated iron-binding capacity

A technology for detecting kits and iron binding force, which is applied in measuring devices, color/spectral characteristic measurement, and material analysis by observing the influence of chemical indicators, etc. It can solve the problems of high price and complicated operation, and achieve low price , improved stability, accuracy and stability with good results

Inactive Publication Date: 2017-02-01
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Theoretically, this method is more direct and the result is more accurate. However, because the determination of serum transferrin mostly uses immunological methods, which are expensive and complicated to operate, the method based on iron ion determination is still the most clinically effective method. common

Method used

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  • Detection kit with stable unsaturated iron-binding capacity
  • Detection kit with stable unsaturated iron-binding capacity
  • Detection kit with stable unsaturated iron-binding capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A stable unsaturated iron-binding capacity detection kit, which includes reagent R1 and reagent R2.

[0025] Wherein the reagent R1 consists of:

[0026] pH 8.6 AMP buffer 100mmol / L

[0027] Thiourea 50mmol / L

[0028] Ferrous ammonium sulfate 20mmol / L

[0029] Hydroxylamine hydrochloride 200mmol / L

[0030] Span80 1%

[0031] Sodium azide 10mmol / L

[0032] Reagent R2 consists of:

[0033] Glycine buffer at pH 3.2 20mmol / L

[0034] Ferene 2mmol / L

[0035] Ascorbic acid 20mmol / L

[0036] Span80 1%

[0037] Sodium azide 10mmol / L.

[0038] The reagents R1 and R2 described in this example need to be prepared by adding buffer substances first, and then adding other components after adjusting to an appropriate pH value. The kit described in the present embodiment, when in use, its measuring method is to adopt Mindray 800 automatic biochemical analyzer with double reagent function, utilize rate method to measure, and detect main wavelength to be 600nm, operate as foll...

Embodiment 2

[0043] Accuracy verification test: the unsaturated iron binding capacity detection reagent of Example 1 was used as the experimental group, and a kind of unsaturated iron binding capacity detection kit with good accuracy and good stability recognized on the market was used as the control group for detection. 20 clinical serum samples were tested, and the test results were as follows: image 3 shown. Correlation curves for the two reagents were obtained (eg figure 1 Shown), the results show that the correlation coefficient of the two kits is 0.9970, indicating that the correlation between the two is relatively good. It proves that the added and changed components of the kit of the present invention will not affect its accuracy, and the kit still maintains a good accuracy.

Embodiment 3

[0045] Linear correlation verification test: Prepare high-value samples with an iron content of 80 μmol / L, serially dilute them with normal saline, and prepare 6 samples with different concentrations, the concentrations are 80 μmol / L, 64 μmol / L, 48 μmol / L, 32 μmol / L L, 16 μmol / L, 0 μmol / L. Utilize respectively embodiment 1 reagent and control group reagent to detect, the sample of each concentration is measured three times respectively, get average value respectively, and detection result is as follows: Figure 4 shown.

[0046] Such as Figure 4 Shown, the correlation coefficient of embodiment 1 and the detection result of matched group reagent is all greater than 0.990, and the correlation coefficient of embodiment 1 reagent detection result is slightly greater than the correlation coefficient of control group reagent detection result, this shows that reagent of the present invention has better linear correlation sex.

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Abstract

The invention discloses a detection kit with stable unsaturated iron-binding capacity, belonging to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1 and a reagent R2. The buffer solution of the reagent R1 is replaced with an AMP (adenosine monophosphate) buffer solution with the pH value of 8.6, and ascorbic acid is added into the reagent R2, thereby enhancing the stability of the kit; and the kit has favorable linear dependence and high reagent accuracy, and is beneficial to further popularization and application in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a detection kit for unsaturated iron binding capacity. Background technique [0002] The iron content of a normal human body is about 4g, of which 2 / 3 exists in the hemoglobin in red blood cells, and the remaining 1 / 3 is stored in the liver, spleen and bone marrow. All iron ions in serum are combined with transferrin, which is the transport form of iron ions, called serum iron. Usually, only about 30% of serum transferrin is combined with iron ions, and about 70% is not combined with iron ions. This potential ability to bind iron is called unsaturated iron binding capacity (UIBC). The maximum amount of iron that can be bound by transferrin in serum is called total iron binding capacity (TIBC). Total iron binding capacity is equal to the sum of serum iron and unsaturated iron binding capacity. Clinically, TIBC and serum iron are usually measured to e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31G01N2021/3185
Inventor 谭柏清胡晓飞李敏王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD