Immune lateral chromatography detection system and preparation method thereof

A technology of immune lateral chromatography and detection system, which is applied in the field of immune lateral chromatography detection system and its preparation, can solve problems such as increased product cost, high operation requirements, and troublesome production, so as to improve efficiency and accuracy, reduce Production cost and the effect of reducing the production process

Inactive Publication Date: 2017-02-01
北京康思润业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing immunological lateral flow method generally soaks or sprays markers in or on special marker pads, which has the following disadvantages: there are many components, which affect the creeping water of the product and the release of markers; Due to the large number of components, the production is troublesome and labor-intensive, which indirectly increases the cost of the product and is a great waste of social resources.
There are also some markers that are placed in the fluorescent pipette head. This method requires high operation and makes it inconvenient.
Large quantities of markers are used, leading to high production costs

Method used

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  • Immune lateral chromatography detection system and preparation method thereof
  • Immune lateral chromatography detection system and preparation method thereof
  • Immune lateral chromatography detection system and preparation method thereof

Examples

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Embodiment 1

[0044] A preparation method of an immune lateral chromatography system, comprising the steps of:

[0045] (1) Dilute CRP detection antibody 1 with coating buffer to 2.0 mg / ml, and control antibody 1 (goat anti-chicken IgY) is also diluted to 2.0 mg / ml, and use special coating equipment to coat the above two liquids The detection zone and the quality control zone were respectively formed on the nitrocellulose membrane (NC) of Sartorius, and dried in a 37° C. oven for 4 hours. The coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as a protective agent.

[0046] (2) Seal the nitrocellulose membrane: immerse in membrane treatment solution A for 1 hour, then soak in membrane treatment solution B for 20 minutes, and dry.

[0047] Wherein, the membrane treatment solution A includes the following components: wherein CB is the molar concentration, and the rest is the mass percentage, and the specific formula is as follows: 50mMol / l of CB, 0.01% of BSA, 0.4% o...

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Abstract

The invention discloses an immune lateral chromatography detection system and a preparation method thereof. The detection system comprises a bottom plate; the bottom plate comprises a near sample end and a far sample end; the bottom plate is sequentially provided with a sample pad, a nitrocellulose membrane and a water absorption pad in a connecting way from the near sample end to the far sample end; the nitrocellulose membrane is provided with a marker band; a detection band and a quality control band are arranged between the marker band and the water absorption pad. The immune lateral chromatography detection system has the advantages of being low in cost and accurate in detection.

Description

technical field [0001] The invention relates to the medical field, in particular to an immunological lateral flow detection system and a preparation method thereof. Background technique [0002] Immunolateral flow-through diagnostic technology is a stable and practical technology suitable for use in various point-of-care tests (POCT) or on-site. According to the labeling part, it is mainly divided into colloidal gold immunological lateral flow chromatography, ordinary fluorescent immunological lateral flow chromatography, time resolved fluorescent Directional chromatography, etc., according to the coating part, there are mainly nitrocellulose membrane, composite material (fusion5), microfluidics, etc. [0003] With the development of immunochromatography (immunochromatography) and colloidal gold technology, especially after the 1990s, colloidal gold immunochromatography (Gold immunochromatography assay, GICA) has been widely used in in vitro diagnostic disease detection. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533
CPCG01N33/558G01N33/533
Inventor 王龙
Owner 北京康思润业生物技术有限公司
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