Preparation method for extracting high-purity chicoric acid from echinacea purpurea herb

A technology of cichoric acid and echinacea, which is applied in the field of preparation of extracting high-purity cichoric acid, and achieves the effects of good anti-inflammatory, high extraction yield, and reduced microwave demand

Active Publication Date: 2017-02-08
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current enzymatic hydrolysis reactions need

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] A preparation method for extracting high-purity cichoric acid from Echinacea purpurea, the steps are as follows:

[0022] 1) at 25 o C, weigh 75 g of citric acid solution with pH=6, add 5 g of β-glucosidase while stirring, after it is completely dissolved, slowly add 10 g of 2,4,6-trinitrobenzenesulfonic acid, and react at constant temperature 8 h, then add 10 g trehalose, and obtain the modified β-glucosidase solution after dissolving;

[0023] 2) Take by weighing 10g of Echinacea purpurea roots after drying and pulverizing pretreatment, put into conical flask, add 90 g ethanol aqueous solution, wherein the concentration of ethanol aqueous solution is 15%, obtain Echinacea purpurea root dispersion liquid;

[0024] 3) Put the Erlenmeyer flask containing the dispersion of Echinacea purpurea root into the microwave extractor, add 0.03g of the modified β-glucosidase solution prepared in step 1, so that the enzyme concentration in the extract is 15 mg / L, and extract it at ...

Embodiment 2

[0043] A preparation method for extracting high-purity cichoric acid from Echinacea purpurea, the specific steps are as follows:

[0044] 1) at 25 o C, weigh 70 g of citric acid solution with pH=6, add 10 g of β-glucosidase while stirring, after it is completely dissolved, slowly add 10 g of 2,4,6-trinitrobenzenesulfonic acid, and react at constant temperature 4 h, then add 10 g trehalose, and obtain the modified β-glucosidase solution after dissolving;

[0045] 2) Weigh 5g of Echinacea purpurea root after drying and pulverizing pretreatment, put it into a conical flask, add 95 g ethanol aqueous solution, wherein the concentration of ethanol aqueous solution is 10%, and obtain the echinacea purpurea root dispersion;

[0046] 3) Put the Erlenmeyer flask containing the Echinacea purpurea root dispersion into a microwave extractor, add 0.01g of the modified ß-glucosidase solution prepared in step 1, so that the enzyme concentration in the extract is 10 mg / L, and extract it at 50...

Embodiment 3

[0051] A preparation method for extracting high-purity cichoric acid from Echinacea purpurea, the specific steps are as follows:

[0052] 1) at 25 o C, weigh 86 g of citric acid solution with pH=6, add 2 g of β-glucosidase while stirring, after it is completely dissolved, slowly add 6 g of 2,4,6-trinitrobenzenesulfonic acid, keep the temperature React for 12 h, then add 6 g of trehalose, and dissolve to obtain a modified β-glucosidase solution;

[0053] 2) Weigh 2 g of Echinacea purpurea root after drying and pulverizing pretreatment, put it into a conical flask, add 98 g ethanol aqueous solution, wherein the concentration of ethanol aqueous solution is 30%, and obtain the echinacea purpurea root dispersion;

[0054] 3) Put the Erlenmeyer flask containing the Echinacea purpurea root dispersion into the microwave extractor, add 0.025 g of the modified ß-glucosidase solution prepared in step 1, so that the enzyme concentration in the extract is 5 mg / L, and the concentration is ...

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Abstract

The invention relates to a preparation method for extracting the high-purity chicoric acid from echinacea purpurea herb. The method is characterized by comprising the steps of preparing a modified glucoside enzyme solution; 2) crushing, filtering and pre-processing a dried echinacea root, and then adding an ethanol aqueous solution to obtain an echinacea root ethanol dispersion solution; 3) putting an erlenmeyer flask containing the ethanol aqueous solution to obtain an echinacea root ethanol dispersion solution into a microwave extraction apparatus, adding the modified glucoside enzyme solution, and conducting the microwave heating process for 30 to 90 seconds; 4) after the microwave enzymatic hydrolysis process, subjecting an obtained enzymatic hydrolysate to the high-temperature enzyme deactivation treatment for 10 to 30 minutes to obtain a chicoric acid primary extraction liquid; 5) filtering and concentrating in the vacuum state to obtain the chicoric acid. According to the invention, the purity of the obtained chicoric acid product is larger than 95%, and the yield thereof is larger than 1.2%. Meanwhile, the extraction yield is high.

Description

technical field [0001] The invention belongs to the technical field of natural product preparation, in particular to a preparation method for extracting high-purity cichoric acid from Echinacea purpurea. Background technique [0002] The genus Echinacea (Echinacea) is a kind of wild flower of Asteraceae native to America. In recent years, studies have shown that its main active ingredient, cichoric acid, can inhibit the destruction of hyaluronidase and reduce the production of toxic products in the intercellular matrix. The diffusion rate between them achieves the anti-inflammatory effect. With the continuous emergence of environmental problems, people pay more and more attention to skin inflammation, and the market demand for anti-inflammatory cosmetics is gradually expanding. Obtaining high-purity cichoric acid and applying it in cosmetics will bring huge economic benefits . [0003] However, most of the existing cichoric acid extraction processes have disadvantages such...

Claims

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Application Information

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IPC IPC(8): C07C67/48C07C67/58C07C69/732
CPCC07C67/48C07C67/58C07C69/732
Inventor 晏青陈玉燕王丽娜张甜海陶柳建菲蒋丽刚
Owner PROYA COSMETICS
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