Method for improving seed germination rate of large flower type paphiopedilum and cultivation method of large flower type paphiopedilum

A technology of germination rate and germination medium, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of hybrid seeds without endosperm or incomplete embryo development, difficulty in large-scale production and utilization, and protocorm browning. It can improve the survival rate of seedlings, shorten the production cycle and improve the germination rate of seeds.

Active Publication Date: 2017-02-15
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the native species of small-flowered or multi-flowered Paphiopedilum, although the commercially-used large-flowered Paphiopedilum has much higher ornamental value, this type of Paphiopedilum is a hybrid with complex ploidy, and often only a few can be obtained. Fewer seeds are difficult to produce and utilize on a large scale, and the seed germination rate is lower than that of native species of small-flowered or multi-flowered Paphiopedil

Method used

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  • Method for improving seed germination rate of large flower type paphiopedilum and cultivation method of large flower type paphiopedilum
  • Method for improving seed germination rate of large flower type paphiopedilum and cultivation method of large flower type paphiopedilum
  • Method for improving seed germination rate of large flower type paphiopedilum and cultivation method of large flower type paphiopedilum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Choose healthy and mature meat pie series Paphiopedilum species (see figure 1 left), artificial cross breeding. When pollinating, the lip petals of the parents are first removed, the pollen grains of the male parent are picked out with a needle, and placed on the stigma of the female parent. Because the pollen clusters have sticky substances, the pollen grains are easy to stick to the stigma. Select pods with different maturity levels from 30 days to 300 days after pollination, and sow them on the seed germination medium respectively. The medium formula is 1 / 4MS+1mg / L6-BA+0.1mg / L NAA+1g / L peptone+25g / L banana+5.5g / L agar+1g / L activated carbon, and count the germination rate after 60 days.

[0045] Depend on image 3 The statistical results show that there is a direct relationship between the germination rate of the meat pie series Paphiopedilum and the degree of seed maturity. The seeds on the inoculated medium did not germinate 120 days after pollination, the highe...

Embodiment 2

[0047] Use the tissue culture seedling of the meat pie series Paphiopedilum variety described in embodiment 1, after going out of bottle, be cultivated in 1.7 cun transparent nutrient pots, and substrate is bark. Set different curing humidity management conditions: the humidity is 60% and 80%. After 200 days of culture, the survival rate was counted. Test results such as Figure 4 It was shown that Paphiopedilum had poor resistance and low survival rate. When the relative humidity is 80%, the survival rate of meatloaf tissue culture seedlings can reach 79%. When the relative humidity was 60%, the survival rate of the meatloaf tissue cultured seedlings was 51%. Therefore, a relative humidity of 80% is more suitable for the growth of tissue culture seedlings out of bottles.

Embodiment 3

[0049]Using the meatloaf series Paphiopedilum varieties described in Example 1, the seed germination rate test was carried out in the same manner as in Example 1, except that the following seed germination medium was used. The result is shown below.

[0050]

[0051] As can be seen from the table above, use 1 / 4MS+1mg / L 6-BA+0.1mg / L NAA+1g / L peptone+25g / L banana+5.5g / L agar+1g / L activated carbon as the seed germination medium It can significantly improve the seed germination rate of Paphiopedilum grandiflorum.

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Abstract

The invention relates to a method for improving the seed germination rate of large flower type paphiopedilum and a cultivation method of the large flower type paphiopedilum. More specifically, the invention provides the method for improving the seed germination rate of the large flower type paphiopedilum. The method comprises the following steps of (1), carrying out artificial pollination after the large flower type paphiopedilum blooms; (2), obtaining seeds after 180 days to 240 days after the pollination and sowing the seeds on a seed germination culture medium, so as to obtain sprout bodies from the seeds. The invention also provides the cultivation method of the large flower type paphiopedilum. According to the cultivation method, the seed germination rate is improved by adopting the method for improving the germination rate. By adopting the methods provided by the invention, the large flower type paphiopedilum of which the flower diameter is greater than 12cm and even reaches 15cm to 16cm can be cultivated; the inflorescence is complete; petals are intact; the ornamental effect is good; moreover, the sowing germination rate is high; the growth speed of a tissue-culture seedling is quick; the survival rate reaches 85 percent or above; the cultivation period can be decreased to 3 years.

Description

technical field [0001] The invention belongs to the fields of flower cultivation and horticulture, and relates to a breeding and cultivation method of Paphiopedilum, more specifically, the invention relates to a breeding and cultivating method of Paphiopedilum, especially a large-flowered Paphiopedilum. Background technique [0002] Paphiopedilum sells well in the international flower market and is also an endangered group of Orchidaceae. Our country's apricot yellow and Paphiopediphyllum have bravely won the overall championship of the world orchid exhibition, and won nearly 100 awards in the American orchid exhibition. There are a total of 79 wild species of Paphiopedilum, about 1 / 3 of which are produced in my country. It is the only endangered wild animal and plant species in the Orchidaceae protected by the Convention on International Trade (CITES). There is a large demand gap in my country's paphiopedilum flower market, and the price is extremely expensive. The price p...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/00A01H4/001A01H4/005
Inventor 刘学庆孙纪霞郭文姣张英杰张京伟刘述河丁朋松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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