Method for preparing fagopyrum tataricum tea by fermenting fagopyrum tataricum stalks and leaves through aspergillus niger

A technology for fermentation of Aspergillus niger and tartary buckwheat tea, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve strong antioxidant activity, simple preparation method, and the effects of preventing and relieving arteriosclerosis

Inactive Publication Date: 2017-02-15
CHENGDU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a method for preparing tartary buckwheat tea by using Aspergillus niger to ferment tartary buckwheat stems and leaves, which can effectively solve the problem of wasting tartary buckwheat stems and leaves, and also adds new benefits to tartary buckwheat tea. type

Method used

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  • Method for preparing fagopyrum tataricum tea by fermenting fagopyrum tataricum stalks and leaves through aspergillus niger
  • Method for preparing fagopyrum tataricum tea by fermenting fagopyrum tataricum stalks and leaves through aspergillus niger
  • Method for preparing fagopyrum tataricum tea by fermenting fagopyrum tataricum stalks and leaves through aspergillus niger

Examples

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Embodiment 1

[0033] Utilize aspergillus niger to ferment tartary buckwheat stem and leaf to prepare the method for tartary buckwheat tea, comprising the following steps:

[0034] (1) Isolation and purification of Aspergillus niger

[0035] Dry Pu-erh tea, weigh 1g, soak it with 100mL of 0.9% sterile physiological saline, use the soaking solution as the mother solution, and then carry out 10-fold concentration gradient dilution of the mother solution until the concentration is 10 -7 g / mL, draw 100 μL of each concentration of soaking solution, spread it on the PDA plate medium, culture it at 25°C for 1-2 days, then pick the colony, separate, purify, and identify it to obtain Aspergillus niger;

[0036] (2) Preparation of Aspergillus niger spore suspension

[0037] Inoculate the Aspergillus niger obtained by the separation and purification of step (1) into the PDA test tube culture medium, cultivate it for 3-5 days at 25°C to produce mature Aspergillus niger spores, and then wash the culture...

Embodiment 2

[0043] Utilize aspergillus niger to ferment tartary buckwheat stem and leaf to prepare the method for tartary buckwheat tea, comprising the following steps:

[0044] (1) Isolation and purification of Aspergillus niger

[0045] Dry the dark tea, weigh 1g, soak it with 100mL of 0.9% sterile physiological saline, use the soaking solution as the mother solution, and then carry out 10-fold concentration gradient dilution of the mother solution until the concentration is 10 -7 g / mL, draw 100 μL of each concentration of soaking solution, spread it on the PDA plate medium, and culture it at 30°C for 1-2 days, then pick the colony, separate, purify, and identify it to obtain Aspergillus niger;

[0046] (2) Preparation of Aspergillus niger spore suspension

[0047] Inoculate the Aspergillus niger obtained by the isolation and purification of step (1) into the PDA test tube culture medium, and cultivate it for 3-5 days at 30°C to produce mature Aspergillus niger spores, then wash the cu...

Embodiment 3

[0053] Utilize aspergillus niger to ferment tartary buckwheat stem and leaf to prepare the method for tartary buckwheat tea, comprising the following steps:

[0054] (1) Isolation and purification of Aspergillus niger

[0055]Dry Pu-erh tea, weigh 1g, soak it with 100mL of 0.9% sterile physiological saline, use the soaking solution as the mother solution, and then carry out 10-fold concentration gradient dilution of the mother solution until the concentration is 10 -7 g / mL, draw 100 μL of each concentration of soaking solution, spread it on the PDA plate medium, culture it at 28°C for 1-2 days, then pick the colony, separate, purify, and identify it to obtain Aspergillus niger;

[0056] (2) Preparation of Aspergillus niger spore suspension

[0057] Inoculate the Aspergillus niger obtained by the separation and purification of step (1) into the PDA test tube culture medium, cultivate it for 3-5 days at 28°C to produce mature Aspergillus niger spores, and then wash the culture ...

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Abstract

The invention provides a method for preparing fagopyrum tataricum tea by fermenting fagopyrum tataricum stalks and leaves through aspergillus niger. The tea comprises the following steps of (1) separating and purifying the aspergillus niger; (2) preparing aspergillus niger spore suspension; (3) taking fagopyrum tataricum stalks and leaves, cleaning the taken fagopyrum tataricum stalks and leaves, drying the cleaned fagopyrum tataricum stalks and leaves, crushing the dried fagopyrum tataricum stalks and leaves to obtain fagopyrum tataricum stalk and leaf powder, sterilizing the fagopyrum tataricum stalk and leaf powder, cooling the sterilized fagopyrum tataricum stalk and leaf powder, adding the aspergillus niger spore suspension to the sterilized fagopyrum tataricum stalk and leaf powder in the volume percentage of the aspergillus niger spore suspension being 3-8% to obtain a mixture, adding sterile water to the mixture, wherein the weight of the sterile water is equal to that of the mixture, uniformly mixing the mixture with the sterile water, and performing fermentation at 28 DEG C for 2-10 days so as to obtain fagopyrum tataricum stalk and leaf fermentation liquor; and (4) baking the fagopyrum tataricum stalk and leaf fermentation liquor obtained in the step (3) at 60 DEG C so as to obtain fagopyrum tataricum stalk and leaf fermenting substances namely the fagopyrum tataricum tea. According to the method disclosed by the invention, the fagopyrum tataricum stalks and leaves obtained after harvesting fagopyrum tataricum seed granules are used as raw materials, and the aspergillus niger as a dominant strain in Pu'er tea or black tea is used for fermenting the fagopyrum tataricum stalks and leaves, so that the problem that the fagopyrum tataricum stalks and leaves are abandoned and incinerated can be effectively solved, and besides, a new kind of the fagopyrum tataricum tea is also increased.

Description

technical field [0001] The invention belongs to the technical field of tartary buckwheat tea, and in particular relates to a method for preparing tartary buckwheat tea by using aspergillus niger to ferment tartary buckwheat stems and leaves. Background technique [0002] Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn), an annual plant belonging to the dicotyledonous Polygonaceae, is a kind of miscellaneous grain resource with an absolute advantage in China. "Compendium of Materia Medica", "Revisiting Zhenghe Zhenglei Materia Medica", "Dictionary of Traditional Chinese Medicine" and other books, "Buckwheat is bitter in nature and taste, flat, cold, good for energy, spirit, eyes and ears, lowering qi, widening intestines and stomach. Function"; "leaves are used as food, lower qi, benefit eyes and ears"; "cure choking food, carbuncle, and can stop bleeding and eat flesh". Studies on pharmacological and chemical components have shown that tartary buckwheat contains rich nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/34C12N1/14C12P1/02C12R1/685
CPCA23F3/34C12N1/14C12P1/02C12N1/145C12R2001/685
Inventor 雨田陈江张晓喻赵钢邹亮郑瑾
Owner CHENGDU UNIV
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